1887

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Volume 13, Issue 3

TRANSFER OF GENTAMICIN RESISTANCE BETWEEN CULTURES OF IN NUTRIENT BROTH, SERUM AND URINE Free

Abstract

SUMMARY

Only one of 23 gentamicin-resistant cultures of Staphylococcus aureus transferred its resistance in mixed culture in broth to the non-lysogenic S. aureus strain 1030; the transfer-frequency was 10–10. Transfer between non-lysogenic clones of strain 1030 occurred at a similar frequency in urine, and at a frequency of ~101 in serum.

The resistance determinant for transfer between non-lysogenic clones was usually linked to phage genome, as shown by the possession by resistant recipients of immunity to typing phage 75, plaque-forming particles in their culture filtrates and inducibility by mitomycin C. Stability of the resistance on storage and transduction kinetics suggested that these genes were chromosomal.

Two resistant derivatives were isolated that had lost some phage functions and were unable to transfer their resistance further. The epidemiology of gentamicin resistance may in part be explicable by the transient formation of an auto-transmissible element with subsequent integration of the resistance genes into a variety of replicons (i.e., transposition).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© J. MED. MICROBIOL. 1980
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2024-04-20
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References

  1. Bint A. J., George R. H., Healing D. E., Wise R., Davies M. 1977; An outbreak of infection caused by a gentamicin-resistant Staphylococcus aureus. J. clin. Path. 30:165
     [Google Scholar]
  2. Buckwold F. J., Albritton W. L., Ronald A. R., Lertzman J., Henriksen R. 1979; Investigations of the occurrence of gentamicin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother 15:152
     [Google Scholar]
  3. Dowding J. E. 1977; Mechanisms of gentamicin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother 11:47
     [Google Scholar]
  4. Faden H., Neter E., McLaughlin S., Giacoia G. 1979; Gentamicin-resistant Staphylococcus aureus. Emergence in an intensive care nursery. J. Am. med. Ass 241:143
     [Google Scholar]
  5. Greenhood G. P., Hill D., Dixon R. E., Carter M. J., Kanto W. P. 1979; Changing phage typing patterns of epidemic gentamicin-resistant Staphylococccus aureus. Evidence for transmission of gentamicin resistance. Lancet 1:289
     [Google Scholar]
  6. Lacey R. W. 1971; High-frequency transfer of neomycin resistance between naturally occurring strains of Staphylococcus aureus. J. med. Microbiol 4:73
     [Google Scholar]
  7. Lacey R. W. 1972; Genetic control in methicillin-resistant strains of Staphylococcus aureus. J. med. Microbiol 5:497
     [Google Scholar]
  8. Lacey R. W. 1975; Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bact. Rev 39:1
     [Google Scholar]
  9. Lacey R. W. 1980; Plasmid transfer in Staphylococcus aureus: evidence for transduction and conjugation. J. gen. Microbiol in the press
    [Google Scholar]
  10. Lewis S. A., Altemeier W. A. 1978; Emergence of clinical isolates of Staphylococcus aureus resistant to gentamicin and correlation of resistance with bacteriophage type. J. infect. Dis 137:314
     [Google Scholar]
  11. Michel J., Stessman J., Sacks T. 1978; Phenotypic variations in gentamicin-resistant isolates of Staphylococcus aureus. Chemotherapy 24:314
     [Google Scholar]
  12. Naidoo J., Noble W. C. 1978; Transfer of gentamicin resistance between strains of Staphylococcus aureus on skin. J. gen. Microbiol 107:391
     [Google Scholar]
  13. Novick R. 1967; Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33:155
     [Google Scholar]
  14. Phillips S., Novick R. P. 1979; Tn554–a site specific repressor-controlled transposon in Staphylococcus aureus. Nature, London 278:476
     [Google Scholar]
  15. Porthouse A., Brown D. F. J., Smith R. G., Rogers T. 1976; Gentamicin resistance in Staphylococcus aureus. Lancet 1:20
     [Google Scholar]
  16. Saxe M. de, Porthouse A. 1979; Gentamicin resistance in Staphylococcus aureus. Lancet 11:370
     [Google Scholar]
  17. Scott D. F., Wood D. O., Brownell G. H., Carter M. J., Best G. K. 1978; Aminoglycoside modification by gentamicin-resistant isolates of Staphylococcus aureus. Antimicrob. Agents Chemother 13:641
     [Google Scholar]
  18. Shanson D. C., Kensit J. G., Duke R. 1976; Outbreak of hospital infection with a strain of Staphylococcus aureus resistant to gentamicin and methicillin. Lancet 2:1347
     [Google Scholar]
  19. Soussy C. J., Dublanchet A., Cormier M., Bismuth R., Mizon F., Chardon H., Duval J., Fabiani G. 1975a; Nouvelles resistances plasmidiques de Staphylococcus aureus aux aminosides. Nouv. Presse med 5:2599
     [Google Scholar]
  20. Soussy C. J., Bouanchaud D. H., Fouace J., Dublanchet A., Duval J. 1975b; A gentamicin resistance plasmid in Staphylococcus aureus. Annls Microbiol., Inst. Pasteur 126B:91
     [Google Scholar]
  21. Speller D. C. E., Raghunath D., Stephens M., Viant A. C., Reeves D. S., Wilkinson P. J., Broughall J. M., Holt H. A. 1976; Epidemic infection by a gentamicin-resistant Staphylococcus aureus in three hospitals. Lancet 1:464
     [Google Scholar]
  22. Warren R. E., Roberts S. O. B. 1976; Gentamicin-resistant staphylococci. Lancet 1:543
     [Google Scholar]
  23. Wood D. O., Carter M. J., Best G. K. 1977; Plasmid-mediated resitance to gentamicin in Staphylococcus aureus. Antimicrob. Agents Chemother 12:513
     [Google Scholar]
  24. Wyatt T. D., Ferguson W. P., Wilson T. S., McCormick E. 1977; Gentamicin resistant Staphylococcus aureus associated with the use of topical gentamicin. J. antimicrob. Chemother 3:213
     [Google Scholar]
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TRANSFER OF GENTAMICIN RESISTANCE BETWEEN CULTURES OF STAPHYLOCOCCUS AUREUS IN NUTRIENT BROTH, SERUM AND URINE
J. Med. Microbiol. 13, 411 (1980); https://doi.org/10.1099/00222615-13-3-411
/content/journal/jmm/10.1099/00222615-13-3-411
/content/journal/jmm/10.1099/00222615-13-3-411
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