f THE CARBON DIOXIDE REQUIREMENTS OF ANAEROBIC BACTERIA
- By Sheena Reilly*
- First Published Online: 01 November 1980, Journal of Medical Microbiology 13: 573-579, doi: 10.1099/00222615-13-4-573
- Subject: Articles
- Issue Published:
FOR ALMOST a century, experimental evidence concerning the importance of carbon dioxide for bacterial growth has been accumulating (Winogradsky, 1890; Rockwell, 1923; Rockwell and Highberger, 1927; Valley and Rettger, 1927; Valley, 1928; Gladstone, Fildes and Richardson, 1935; Wimpenny, 1969). The attention of early workers was directed primarily at aerobes such as Neisseria gonorrhoeae (Chapin, 1918), N. meningitidis (Cohen and Fleming, 1918), Brucella abortus (Smith, 1924; Wilson, 1931) and Mycobacterium tuberculosis (Wherry and Ervin, 1918; Davies, 1940). With the recent resurgence of interest in anaerobic bacteriology, the essential or stimulatory effect of CO2 on the growth of obligate anaerobes has become widely recognised. Current anaerobic culture techniques commonly employ 5-10% CO2 in the incubation atmosphere to achieve optimal growth (Watt, 1973; Stalons, Thornsberry and Dowell, 1974; Willis, 1977). Nevertheless, there is a lack of precise information relating to the quantitative CO2 requirements of many clinically important anaerobic bacteria. Preliminary experiments in this laboratory revealed a wide variation in the CO2 requirements of different species of anaerobic bacteria; the observation that relatively low concentrations of CO2 were adequate for satisfactory growth of certain anaerobes was of particular interest. The present study reports the effect of different concentrations of CO2 on the growth of a range of anaerobic bacteria isolated from clinical sources.
Present address: Public Health Laboratory, General Hospital, Greenbank Road, Plymouth PL4 8NN.
© 1980 The Pathological Society of Great Britain and Ireland | Published by the Microbiology Society
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