@article{mbs:/content/journal/jmm/10.1099/00222615-13-4-573, author = "Reilly, Sheena", title = "THE CARBON DIOXIDE REQUIREMENTS OF ANAEROBIC BACTERIA", journal= "Journal of Medical Microbiology", year = "1980", volume = "13", number = "4", pages = "573-579", doi = "https://doi.org/10.1099/00222615-13-4-573", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-13-4-573", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "SUMMARY Some clinically significant anaerobic bacteria were incubated in pure culture in anaerobic jars containing a range of atmospheric concentrations of CO2. Growth of Clostridium perfringens, C. sporogenes and C. septicum was independent of the amount of CO2. Small supplements of CO2 (0.25%) allowed good growth of the majority of anaerobes studied—an observation contrary to established teaching. The exceptions were Fusobacterium necrophorum which showed an absolute requirement for CO2 of at least 1% and B. melaninogenicus which needed an atmospheric content of 10–40% CO2 for optimal growth. The inclusion of CO2 in the anaerobic jar at a final concentration of 10% is to be recommended for all routine isolation procedures.", }