1887

Journal of Medical Microbiology logo

Volume 14, Issue 1

Chlamydial serum IgG, IgA and local IgA antibodies in patients with genital-tract infections measured by solid-phase radioimmunoassay Free

Abstract

Summary.

A solid-phase radioimmunoassay (RIA) for IgG and IgA class antibodies to was developed with serotype L2 as antigen. The assay was sensitive, reproducible and correlated well with an immunofluorescence test (r = 0·85). Serum IgG antibodies were detected in 79% of isolation-positive versus 43% of isolation-negative male patients with urethritis, and serum IgA antibodies in 53% and 21%, respectively. Urethral IgA antibodies, measured from specimens taken for chlamydial isolation, could be detected in 94% and 38%, respectively. From 737 male urethral and 909 female cervical secretions screened for the presence of IgA antibodies, about half were isolation and IgA negative. Only 4% (6/151) of male and 5·4% (2/37) of female isolation-positive specimens were IgA negative. The determination of local IgA antibodies may be used as a screening test in chlamydial genital infections.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1981
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-14-1-77
1981-02-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/14/1/medmicro-14-1-77.html?itemId=/content/journal/jmm/10.1099/00222615-14-1-77&mimeType=html&fmt=ahah

References

  1. Arstila P., Vuorimaa T., Kalimo K., Halonen P., Viljanen M., Granfors K., Toivanen P. 1977; A solid-phase radioimmunoassay for IgG and IgM antibodies against measles virus. J. gen. Virol. 34:167
     [Google Scholar]
  2. Grayston J. T., Yeh L. J., Wang S. P., Kuo C. C., Beasley R. P., Gale J. L. 1977; Pathogenesis of ocular Chlamydia trachomatis infections in humans. I Nongonococcal urethritis and related infections Hobson D., Holmes K. K. American Society for Microbiology; Washington, DC:113
     [Google Scholar]
  3. Hanna L., Dawson C. R., Briones O., Thygeson P., Jawetz E. 1968; Latency in human infections with TRIC agents. J. Immun 101:43
     [Google Scholar]
  4. Holmes K. K., Handsfield H. H., Wang S. P., Wentworth B. B., Turck M., Anderson J. B., Alexander E. R. 1975; Etiology of nongonococcal urethritis. N. Engl. J. Med. 292:1199
     [Google Scholar]
  5. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, Lond. 194:495
     [Google Scholar]
  6. Jones B. R. 1977; In Nongonococcal urethritis and related infections. Hobson D., Holmes K. K. American Society for Microbiology; Washington, DC:511
     [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
     [Google Scholar]
  8. McComb D. E., Nichols R. L., Semine D. Z., Evrard J. R., Alpert S., Crockett V. A., Rosner B., Zinner S. H., McCormack W. M. 1979; Chlamydia trachomatis in women: antibody in cervical secretions as a possible indicator of genital infection. J. inf. Dis 139:628
     [Google Scholar]
  9. Meurman O. H. 1978; Antibody responses in patients with rubella infection determined by passive hemagglutination, hemagglutination inhibition, complement fixation, and solid-phase radioimmunoassay tests. Infect. Immun. 19:369
     [Google Scholar]
  10. Ng K. M., Graham D. M., Forsyth J. R. L., Brennan K. F. 1978; Antichlamydial antibody in genital exudates of men and women with non-gonococcal genital infections. Lancet 1:507
     [Google Scholar]
  11. Oriel J. D., Johnson A. L., Barlow D., Thomas B. J., Nayyar K. , Reeve P. 1978; Infection of the uterine cervix with Chlamydia trachomatis . J. infect. Dis. 137:443
     [Google Scholar]
  12. Oriel J. D., Reeve P., Thomas B. J., Nicol C. S. 1975; Infection with Chlamydia group A in men with urethritis due to Neisseria gonorrhoeae . J. infect. Dis. 131:376
     [Google Scholar]
  13. Punnonen R., Terho P., Nikkanen V., Meurman O. 1979; Chlamydial serology in infertile women by immunofluorescence. Fert. Steril. 31:656
     [Google Scholar]
  14. Schachter J. 1978a; Chlamydial infections (first of three parts). N. Engl. J. Med. 298:428
     [Google Scholar]
  15. Schachter J. 1978b; Chlamydial infections (third of three parts). N. Engl. J. Med. 298:540
     [Google Scholar]
  16. Schachter J., Cles L., Ray R., Hines P. A. 1979; Failure of serology in diagnosing chlamydial infections of the female genital tract. J. clin. Microbiol. 10:647
     [Google Scholar]
  17. Terho P. 1978a; Chlamydia trachomatis in non-specific urethritis. Brit. J. vener. Dis. 54:251
     [Google Scholar]
  18. Terho P. 1978b; Chlamydia trachomatis in gonococcal and post-gonococcal urethritis. Brit.J. vener. Dis. 54:326
     [Google Scholar]
  19. Terho P. 1978c; Isolation techniques of Chlamydia trachomatis from patients with nonspecific urethritis. Dermatol. Monatsschr. 164:515
     [Google Scholar]
  20. Treharne J. D., Dines R. J., Darougar S. 1977; Serological responses to chlamydial ocular and genital infections in the United Kingdom and Middle East. In Nongonococcal urethritis and related infections Hobson D., Holmes K. K. American Society for Microbiology; Washington, DC:249
     [Google Scholar]
  21. Wang S. P., Grayston J. T. 1970; Immunological relationships between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am. J. Ophthal. 70:367
     [Google Scholar]
  22. Wang S. P., Grayston J. T., Kuo C. C., Alexander E. R., Holmes K. K. 1977; Serodiagnosis of Chlamydia trachomatis infection with the micro-immunofluorescence test. In Nongonococcal urethritis and related infections Hobson D., Holmes K. K. American Society for Microbiology; Washington, DC:237
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-14-1-77
Loading
Chlamydial serum IgG, IgA and local IgA antibodies in patients with genital-tract infections measured by solid-phase radioimmunoassay
J. Med. Microbiol. 14, 77 (1981); https://doi.org/10.1099/00222615-14-1-77
/content/journal/jmm/10.1099/00222615-14-1-77
/content/journal/jmm/10.1099/00222615-14-1-77
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error