1887

Abstract

SUMMARY

The levels of pertussis-specific IgA antibodies in sera from vaccinees and from children with infection were compared by an enzyme-linked immunosorbent assay (ELISA). Serum IgA antibodies were produced only after natural contact with the pathogen and, therefore, their presence can be used as an indicator of infection. However, in view of the relatively long interval between infection and the appearance of antibodies, and the prolonged antibody response, their presence cannot be used as proof of recent infection. The finding of these antibodies in a high percentage of the normal adult population may indicate a constant circulation of without symptoms of disease.

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1983-11-01
2024-04-19
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References

  1. Aleksandrowicz J., Pstragowska W. 1980; Biological activity of serum and secretory IgA in the course of pertussis in children. Medycyna Doswiadczalna i Mikrobiologia 32:201–207 (in Polish)
    [Google Scholar]
  2. Ashworth L. A. E., Fitzgeorge R. B., Irons L. I., Morgan C. P., Robinson A. 1982; Rabbit nasopharyngeal colonization by Bordetella pertussis: the effects of immunization clearance and on serum and nasal antibody levels. Journal of Hygiene 88:475–486
    [Google Scholar]
  3. Bienenstock J., Befus A. D. 1980; Mucosal immunology. Immunology 41:249–270
    [Google Scholar]
  4. Brouwer R., Cohen H. 1976; Reactogenicity for infants of pertussis vaccines, harvested by centrifugation or acid precipitation. Journal of Biological Standardization 4:5–12
    [Google Scholar]
  5. Geller B. D., Pittman M. 1973; Immunoglobulin and histamine-sensitivity response of mice to live Bordetella pertussis. Infection and Immunity 8:83
    [Google Scholar]
  6. Goodman Y. E., Wort A. J., Jackson F. L. 1981; Enzyme-linked immunosorbent assay for detection of pertussis immunoglobulin A in nasopharyngeal secretions as an indicator of recent infection. Journal of Clinical Microbiology 13:286–292
    [Google Scholar]
  7. Granstrom M., Lindberg A. A., Askelof P., Hederstedt B. 1982; Detection of antibodies in human serum against the fimbrial haemagglutinin of Bordetella pertussis by enzyme-linked immunosorbent assay. Journal of Medical Microbiology 15:85–96
    [Google Scholar]
  8. Hagenaars A. M., Kuipers A. J., Nagel J. 1980; Preparation of enzyme-antibody conjugates. In Immunoenzymatic assay techniques edited by Malvano R., Martinus Nijhoff. The Hague: pp 16–27
    [Google Scholar]
  9. Heremans J. F. 1974; Immunoglobulin A. In The Antigens Vol. II edited by Sela M. Academic Press New York: pp 365–522
    [Google Scholar]
  10. Holt L. B. 1972; The pathology and immunology of Bordetella pertussis infection. Journal of Medical Microbiology 5:407–424
    [Google Scholar]
  11. Lagergren J. 1963; The white blood cell count and the erythrocyte sedimentation rate in pertussis. Acta Paediatrica 52:405–409
    [Google Scholar]
  12. Linneman C. C. 1979; Host-parasite interactions in pertussis. In Proceedings of the 3rd International Symposium on Pertussis held in 1978 edited by Manclark C. R., Hill J. C. U.S. Department of Health, Education and Welfare; Bethesda, MD: DHEW Publication No. (NIH) 79–1830 pp 3–18
    [Google Scholar]
  13. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  14. Ogra P. L., Fishaut M., Gallagher M. R. 1980; Viral vaccination via the mucosal routes. Reviews of Infectious Diseases 2:352–369
    [Google Scholar]
  15. Olson L. C. 1975; Pertussis. Medicine. 54:427–469
    [Google Scholar]
  16. Pittman M. 1979; Pertussis toxin: The cause of the harmful effects and prolonged immunity of whooping cough. A hypothesis. Reviews of Infectious Diseases 1:401–412
    [Google Scholar]
  17. Rivat C., Bourguignon J., Fontaine M., Ropartz C. 1977; Enzymatic fragmentation of human IgA F(abc)′2: a new peptic fragment. Immunochemistry 14:69–73
    [Google Scholar]
  18. Ruuskanen O., Mertsola J., Viljanen M. K. 1981; Atypical whooping cough: serological diagnosis and clinical signs. Pediatric Research 15:1185
    [Google Scholar]
  19. Ruuskanen O., Viljanen M. K., Salmi T. T., Lehtonen O. P., Kouvalainen K., Peltonen T. 1980; DTP and DTP-inactivated polio vaccines: Comparison of adverse reactions and IgG, IgM and IgA antibody responses to DTP. Acta Paediatrica Scandinavica 69:177–182
    [Google Scholar]
  20. Sato Y., Arai H., Suzuki K. 1974; Leukocytosis-promoting factor of Bordetella pertussis. III. Its identity with protective antigen. Infection and Immunity 9:801–810
    [Google Scholar]
  21. Thomas G. 1975; Respiratory and humoral immune response to aerosol and intramuscular pertussis vaccine. Journal of Hygiene 74:233–237
    [Google Scholar]
  22. Viljanen M. K., Ruuskanen O., Granberg C., Salmi T. T. 1982; Serological diagnosis of pertussis: IgM, IgA and IgG antibodies against Bordetella pertussis measured by enzyme-linked immunosorbent assay (ELISA). Scandinavian Journal of Infectious Diseases 14:117–122
    [Google Scholar]
  23. Waldman R. H., Ganguly R. 1974; Immunity to infections on secretory surfaces. Journal of Infectious Diseases 130:419–440
    [Google Scholar]
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