1887

Abstract

SUMMARY

Urinary isolates of that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 µg/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 µg/ml; 73% were multiresistant.

Plasmids were detected in 98% of strains and 60% carried two or more. Half of the isolates transferred trimethoprim resistance to K12 and 70% of these cotransferred resistance to sulphonamide although these markers were often not linked. Trimethoprim resistance was carried on 12 different plasmids, four of which also conferred sulphonamide resistance. All except two carried streptomycin resistance which suggests that Tn7 was probably present. The results are discussed in relation to current prescribing policy.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-16-4-433
1983-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/16/4/medmicro-16-4-433.html?itemId=/content/journal/jmm/10.1099/00222615-16-4-433&mimeType=html&fmt=ahah

References

  1. Ackerman V. P., Groot Obbink D. J. 1979; Screening for transferable antibiotic resistance in the clinical laboratory. Journal of Antimicrobial Chemotherapy 5:167–172
    [Google Scholar]
  2. Amyes S. G. B., Emmerson A. M., Smith J. T. 1978; R factor mediated trimethoprim resistance: result of two three-month clinical surveys. Journal of Clinical Pathology 31:850–854
    [Google Scholar]
  3. Amyes S. G. B., Smith J. T. 1978; Trimethoprim antagonists: effect of uridine in laboratory media. Journal of Antimicrobial Chemotherapy 4:421–429
    [Google Scholar]
  4. Bachmann B. J. 1972; Pedigrees of some mutant strains of Escherichia coli K12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  5. Barth P. T., Datta N., Hedges R. W., Grinter N. J. 1976; Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. Journal of Bacteriology 125:800–810
    [Google Scholar]
  6. British Medical Journal (Leading article) 1980; Bacterial resistance to trimethoprim. British Medical Journal 281:571–572
    [Google Scholar]
  7. Broda P. 1979 Plasmids Freeman W. H., Co. Oxford: p 14
    [Google Scholar]
  8. Brogden R. N., Carmine A. A., Heel R. C., Speight T. M., Avery G. S. 1982; Trimethoprim: A review of its antibacterial activity, pharmacokinetics and therapeutic use in urinary tract infections. Drugs 23:405–430
    [Google Scholar]
  9. Burman L. G. 1980; Resistance to trimethoprim. Lancet 1:1409
    [Google Scholar]
  10. Datta N. 7 others 1980; Distribution of genes for trimethoprim and gentamicin resistance in bacteria and their plasmids in a general hospital. Journal of General Microbiology 118:495–508
    [Google Scholar]
  11. Datta N., Nugent M., Richards H. 1981; Transposons encoding trimethoprim or gentamicin resistance in medically important bacteria. Cold Spring Harbor Symposia on Quantitative Biology XLV45
    [Google Scholar]
  12. Datta N., Richards H. 1981; Trimethoprim resistant bacteria in hospital and in the community: spread of plasmids and transposons. In Molecular biology, pathogenicity, and ecology of bacterial plasmids edited by Levy S. B., Clowes R. C., Koenig E. L. Plenum Press New York: pp 21–30
    [Google Scholar]
  13. Fleming M. P., Datta N., Gruneberg R. N. 1972; Trimethoprim resistance determined by R-factors. British Medical Journal 1:726–728
    [Google Scholar]
  14. Hamilton-Miller J. M. T., Gooding A., Brumfitt W. 1981; Resistance to trimethoprim in 1978–79 compared with 1973–75. Journal of Clinical Pathology 34:439–442
    [Google Scholar]
  15. Platt D. J., Sommerville J. S. 1981; A simple method for the detection of resistance plasmids in Serratia species by transfer to members of the genus Enterobacter. Journal of Antimicrobial Chemotherapy 8:145–152
    [Google Scholar]
  16. Salter A. J. 1982; Trimethoprim-sulfamethoxazole: an assessment of more than 12 years of use. Reviews of Infectious Diseases 4:196–236
    [Google Scholar]
  17. Towner K. J., Pearson N. J., Pinn P. A., O’Grady F. 1980; Increasing importance of plasmid mediated trimethoprim resistance in enterobacteria: two six month clinical surveys. British Medical Journal 280:517–519
    [Google Scholar]
  18. Towner K. J., Pinn P. A. 1981; A transferable plasmid conferring only a moderate level of resistance to trimethoprim. FEMS Microbiology Letters 10:271–272
    [Google Scholar]
  19. Willshaw G. A., Smith H. R., Anderson E. S. 1979; Application of agarose gel electrophoresis to the characterization of plasmid DNA in drug resistant enterobacteria. Journal of General Microbiology 114:15–25
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-16-4-433
Loading
/content/journal/jmm/10.1099/00222615-16-4-433
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error