1887

Abstract

Summary

The cross-reactivity of crude sonic extracts of six species of mycobacteria was studied by skin tests in mice and guinea pigs immunised with live strain H37Rv, and in patients with pulmonary tuberculosis. All slowly growing mycobacteria elicited a strong delayed hypersensitivity response, a moderate response and a poor or no response. A specific target antigen for cell-mediated immunity, known to be present in , was also present in all the mycobacteria studied. This shared antigen was shown, by immunoprecipitation tests, to be identical in all the slowly growing species but only a partial reaction of identity was obtained with . It is concluded that the antigen shared by the slowly growing mycobacteria is immunodominant in cell-mediated immunity.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-19-1-69
1985-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/19/1/medmicro-19-1-69.html?itemId=/content/journal/jmm/10.1099/00222615-19-1-69&mimeType=html&fmt=ahah

References

  1. Daniel T M, Janicki B W. 1978; Mycobacterial antigens: A review of their isolation, chemistry, and immunological properties. Microbiological Reviews 42:84–113
    [Google Scholar]
  2. Doub L, Youmans G P. 1950; Studies in tuberculosis chemotherapy. 1. Simple primary aromatic amines, in vitro and in vivo. American Review of Tuberculosis 61:407–421
    [Google Scholar]
  3. Freund J E. 1967; Modern elementary statistics. , 3. Prentice-Hall Inc. Publications; New Jersey:225
    [Google Scholar]
  4. Gray D F, Jennings P A. 1955; Allergy in experimental mouse tuberculosis. American Review of Tuberculosis 72:171–195
    [Google Scholar]
  5. Harboe M, Closs O, Reitan L J, Draper P. 1981; Demonstration of antibodies reacting with different determinants on Mycobacterium leprae antigen 7. International Journal of Leprosy 49:147–158
    [Google Scholar]
  6. Hattikudur S N, Kamat R S. 1984; Characterisation of a target antigen of cell mediated immunity in the sonic extract of M. tuberculosis strain H37Rv. Journal of Medical Microbiology 18:17–25
    [Google Scholar]
  7. Kronvall G, Stanford J L, Walsh G P. 1976; Studies of mycobacterial antigens with special reference to Mycobacterium leprae. Infection and Immunity 13:1132–1138
    [Google Scholar]
  8. Kusunose E, Kusunose M, Ichihara K, Izumi S. 1980; Occurrence of superoxide dismutase in Mycobacterium leprae grown in armadillo liver. Journal of General and Applied Microbiology 26:369–372
    [Google Scholar]
  9. Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951; Protein measurement with the folin-phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Magnusson M. 1961; Specificity of mycobacterial sensitins I. Studies in guinea pigs with purified “tuberculin” prepared from mammalian and avian tubercle bacilli, Mycobacterium balnei and other acid-fast bacilli. American Review of Respiratory Diseases 83:57–68
    [Google Scholar]
  11. Ouchterlony O, Nilsson L A. 1978; Immunodiffusion and Immunoelectrophoresis. Weir D M. Handbook of experimental immunology, 3. Blackwell Scientific Publications; Oxford:19.5
    [Google Scholar]
  12. Roberts D B, Wright G L, Affronti L F, Reich M. 1972; Characterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis. Infection and Im-munity 6:564–573
    [Google Scholar]
  13. Stanford J L, Rook G A W, Convit J, Godal T, Kronvall G, Rees RJW, Walsh G P. 1975; Preliminary taxonomic studies on the leprosy bacillus. British Journal of Experimental Pathology 56:579–585
    [Google Scholar]
  14. Wayne L G. 1976; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium: tests for niacin and catalase and for resistance to isoniazid thiophene 2-carboxylic acid hydrazide, hydroxylamine, and p-nitrobenzoate. International Journal of Systematic Bacteriology 26:311–318
    [Google Scholar]
  15. World Health Organization 1979 Trial of BCG vaccines in South India for tuberculosis prevention–first report, Tuberculosis prevention trial Bulletin of the World Health Organization 57:819–827
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-19-1-69
Loading
/content/journal/jmm/10.1099/00222615-19-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error