f The Detection Of Methicillin Resistance In Staphylococcus Aureus
- Authors: J. H. Hewitt, A. W. Coe, M. T. Parker
- J. Med. Microbiol., November 1969 2: 443-456, doi: 10.1099/00222615-2-4-443
- Subject: Articles
- Published Online:
Some methicillin-resistant cultures of Staphylococcus aureus give equivocal results when tested in disk diffusion tests or tube dilution MIC tests incubated at 37°C.
Practically all resistant cultures have the following characters: (1) they give growth up to or to within 1 mm of a 10 μg methicillin disk in a disk diffusion test on nutrient agar or blood agar incubated at 30°C, and (2) they show a MIC of 25 μg methicillin per ml or greater after incubation for 18 hr at 30°C, or for 48 hr at 37°C, in a tube dilution test in nutrient broth.
Tests for growth at 37°C on methicillin salt agar, or on salt agar with a methicillin disk, are also generally reliable, but some batches of methicillin salt agar are relatively inhibitory for resistant staphylococci.
Routine sensitivity tests with cloxacillin give unsatisfactory results.
©E & S LIVINGSTONE, LTD. 1969 | Published by the Microbiology Society
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