RT Journal Article SR Electronic(1) A1 Hewitt, J. H. A1 Coe, A. W. A1 Parker, M. T.YR 1969 T1 The Detection Of Methicillin Resistance In Staphylococcus Aureus JF Journal of Medical Microbiology, VO 2 IS 4 SP 443 OP 456 DO https://doi.org/10.1099/00222615-2-4-443 PB Microbiology Society, SN 1473-5644, AB Summary Some methicillin-resistant cultures of Staphylococcus aureus give equivocal results when tested in disk diffusion tests or tube dilution MIC tests incubated at 37°C. Practically all resistant cultures have the following characters: (1) they give growth up to or to within 1 mm of a 10 μg methicillin disk in a disk diffusion test on nutrient agar or blood agar incubated at 30°C, and (2) they show a MIC of 25 μg methicillin per ml or greater after incubation for 18 hr at 30°C, or for 48 hr at 37°C, in a tube dilution test in nutrient broth. Tests for growth at 37°C on methicillin salt agar, or on salt agar with a methicillin disk, are also generally reliable, but some batches of methicillin salt agar are relatively inhibitory for resistant staphylococci. Routine sensitivity tests with cloxacillin give unsatisfactory results., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-2-4-443