1887

Abstract

Summary

Aminoglycoside-resistance determinants in staphylococci are borne on conjugative and non-conjugative plasmids. The conjugative plasmids were found in methicillin-resistant strains of isolated recently in Darwin and Sydney, Australia and in Houston, Texas, USA. These plasmids and the class-2 conjugative plasmid reported by Archer and Johnston (1983) had similar patterns of R1 restriction-endonuclease fragments, encoded resistance to gentamicin, kanamycin and neomycin, transferred to a non-lysogenic recipient in conditions that promoted close cell-to-cell contact and mobilised a small, non-conjugative plasmid. A further plasmid, pWG14, encoding resistance to kanamycin, neomycin, streptomycin, erythromycin and lincomycin, also displayed conjugative properties but did not mobilise the small, non-conjugative plasmid. The transfer frequency of all conjugative plasmids was stimulated by the addition of polyethylene glycol, particularly at concentrations above 20%, to mixtures of donor and recipient broth cultures. Polyethylene glycol appeared to promote close cell-to-cell contact between donor and recipient cells. A representative of the most common aminoglycoside-resistance plasmids in Australian isolates of methicillin-resistant was non-conjugative and transferred by a bacteriophage-mediated system to a lysogenic recipient. With the exception of plasmid pWG14, the conjugative plasmids were also transferred by a bacteriophage-mediated system. Furthermore, cultural conditions that favoured conjugative transfer of plasmids inhibited bacteriophage-mediated transfer and . The efficacy of the two transfer systems for analysing the plasmids of gentamicin-resistant, methicillin-resistant isolates of has been compared.

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1985-10-01
2024-03-29
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References

  1. Archer G. L., Mayhall C. G. 1983; Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections. Journal of Clinical Microbiology 18:395–399
    [Google Scholar]
  2. Dowd D., Cafferkey M., Dougan G. 1983; Gentamicin and methicillin resistant Staphylococcus aureus in Dublin hospitals: molecular studies. Journal of Medical Microbiology 16:129–138
    [Google Scholar]
  3. El Solh N., Fouace J. M., Pillet J., Chabbert Y. A. 1981; Plasmid DNA. content of multi-resistant Staphylococcus aureus strains. Annales de Microbiologie 1328:131–156
    [Google Scholar]
  4. Espersen F., Nielsen P. B., Lund K., Sylvest B., Jensen K. 1982; Hospital-acquired infections in a burns unit caused by an imported strain of Staphylococcus aureus with an unusual multi-resistance. Journal of Hygiene, Cambridge 88:535–541
    [Google Scholar]
  5. Forbes B. A., Schaberg D. R. 1983; Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. Journal of Bacteriology 153:627–634
    [Google Scholar]
  6. Gedney J., Lacey R. W. 1982; Properties of methicillin-resistant staphylococci now endemic in Australia. Medical Journal of Australia 1:448–450
    [Google Scholar]
  7. Goering R. V., Ruff E. A. 1983; Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 24:450–452
    [Google Scholar]
  8. Gray G. S., Huang R., T-S, Davies J. 1983; Aminocyclitol resistance in Staphylococcus aureus: presence of plasmids and aminocyclitol-modifying enzymes. Plasmid 9:147–158
    [Google Scholar]
  9. Grubb W. B., Annear DI. 1981; Unstable drug resistance in Staphylococcus aureus M4. Genetical Research, Cambridge 38:217–223
    [Google Scholar]
  10. Grubb W. B., O’ Reilly R. J. 1971; Joint transduction of separate extrachromosomal drug resistance determinants in Staphylococcus aureus El69. Biochemical and Biophysical Research Communications 42:377–383
    [Google Scholar]
  11. Grubb W. B., Townsend D. E., Ashdown N., Annear D. I. 1982; Unique mixed culture transfer in staphylococci. Australian Microbiologist 3:60
    [Google Scholar]
  12. Grubb W. B., Townsend D. E., Greed L. C., Ashdown N., Momoh M. 1983; Characteristics of methicillin-resistant Staphylococcus aureus endemic in Australian hospitals. In Spitzy K. H., Karrer K. eds Proceedings of the 13th International congress of chemotherapy. Vienna, 1983. Egermann. Vienna: pp 23–26
    [Google Scholar]
  13. Jaffe H. W., Sweeney H. M., Nathan C., Weinstein R. A., Kabins S. A., Cohen S. 1980; Identity and interspecific transfer of gentamicin-resistance plasmids in Staphylococcus aureus and Staphylococcus epidermidis. The Journal of Infectious Diseases 141:738–747
    [Google Scholar]
  14. Jaffe H. W., Sweeney H. M., Weinstein R. A., Kabins S. A., Nathan C., Cohen S. 1982; Structural and phenotypic varieties of gentamicin resistance plasmids in hospital strains of Staphylococcus aureus and coagulase-negative staphylococci. Antimicrobial Agents and Chemotherapy 21:773–779
    [Google Scholar]
  15. King K., Brady L., Thomson M., Harkness J. L. 1982; Antibiotic-resistant staphylococci in a teaching hospital. Medical Journal of Australia 2:461–465
    [Google Scholar]
  16. Knutton S. 1979; Studies of membrane fusion. III. Fusion of erythrocytes with polyethylene glycol. Journal of Cell Science 36:61–72
    [Google Scholar]
  17. Lacey R. W. 1971; High-frequency transfer of neomycin resistance between naturally occurring strains of Staphylococcus aureus. Journal of Medical Microbiology 4:73–84
    [Google Scholar]
  18. Lacey R. W., Lord V. L. 1980; Transfer of gentamicin resistance between cultures of Staphylococcus aureus in nutrient broth, serum and urine. Journal of Medical Microbiology 13:411–421
    [Google Scholar]
  19. Lacey R. W., Stokes A. 1979; Studies on recently isolated cultures of methicillin-resistant Staphylococcus aureus. Journal of General Microbiology 114:329–339
    [Google Scholar]
  20. Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. 1984a; Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17:79–89
    [Google Scholar]
  21. Lyon B. R., May J. W., Marshall J. H., Skurray R. A. 1982; Plasmid-mediated antibiotic resistance in methicillin-resistant Staphylococcus aureus. Medical Journal of Australia 1:468–469
    [Google Scholar]
  22. Lyon B. R., May J. W., Skurray R. A. 1983; Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 23:817–826
    [Google Scholar]
  23. Lyon B. R., May J. W., Skurray R. A. 1984b; Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Molecular and General Genetics 193:554–556
    [Google Scholar]
  24. Maroudas N. G. 1975; Polymer exclusion, cell adhesion and membrane fusion. Nature 254:695–696
    [Google Scholar]
  25. McDonnell R. W., Sweeney H. M., Cohen S. 1983; Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy 23:151–160
    [Google Scholar]
  26. McGowan J. E., Terry P. M., Huang T -S R., Houk C. L., Davies J. 1979; Nosocomial infections with gentamicin-resistant Staphylococcus aureus: plasmid analysis as an epidemiologic tool. The Journal of Infectious Diseases 140:864–872
    [Google Scholar]
  27. Naidoo J. 1984; Interspecific co-transfer of antibiotic resistance plasmids in staphylococci in vivo. Journal of Hygiene 93:59–66
    [Google Scholar]
  28. Naidoo J., Noble W. C. 1978; Transfer of gentamicin resistance between strains of Staphylococcus aureus on skin. Journal of General Microbiology 107:391–393
    [Google Scholar]
  29. Novick R. P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. Journal of General Microbiology 33:121–136
    [Google Scholar]
  30. Novick R. P., Bouanchaud D. 1971; Extrachromosomal nature of drug resistance in Staphylococcus aureus. Annals of the New York Academy of Sciences 182:279–294
    [Google Scholar]
  31. Pavillard R. et al. 1982; Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Medical Journal of Australia 1:451–454
    [Google Scholar]
  32. Porthouse A., Brown D., F J, Smith R. G., Rogers T. 1976; Gentamicin resistance in Staphylococcus aureus. Lancet 1:20–21
    [Google Scholar]
  33. Scopetti F., Orefici G., Biondi F., Benini F. 1983; Staphylococcus aureus resistant to methicillin and gentamicin as a cause of an outbreak of epidemic enteritis in a hospital. Bollettino Dell’Istituto Sieroterapico Milanese 62:406–411
    [Google Scholar]
  34. Shalita Z., Murphy E., Novick R. P. 1980; Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships. Plasmid 3:291–311
    [Google Scholar]
  35. Townsend D. E., Ashdown N., Bradley J. M., Pearman J. W., Grubb W. B. 1984a; “Australian” methicillin-resistant Staphylococcus aureus in a London hospital?. Medical Journal of Australia 144:339–340
    [Google Scholar]
  36. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984b; Analysis of plasmids mediating gentamicin resistance in methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 13:347–352
    [Google Scholar]
  37. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984c; Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. Journal of Antimicrobial Chemotherapy 14:115–124
    [Google Scholar]
  38. Townsend D. E., Ashdown N., Momoh M., Grubb W. B. 1984 d; Distribution of plasmid-borne resistance to nucleic acid binding compounds in methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy, 15:417–434
    [Google Scholar]
  39. Townsend D. E., Grubb W. B., Ashdown N. 1983a; Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology 15:169–174
    [Google Scholar]
  40. Townsend D. E., Grubb W. B., Ashdown N. 1983b; Genetics of drug resistance in methicillin-resistant Staphylococcus aureus from Australian hospitals. Journal of Hospital Infection 4:331–337
    [Google Scholar]
  41. Vogel L., Nathan C., Sweeney H. M., Kabins S. A., Cohen S. 1978; Infections due to gentamicin-resistant Staphylococcus aureus strain in a nursery for neonatal infants. Antimicrobial Agents and Chemotherapy 13:466–472
    [Google Scholar]
  42. Witte W. 1981; Transfer of drug-resistance plasmids in mixed cultures of staphylococci. In Jeljaszewicz J. ed Staphylococci and staphylococcal infections, Gustav Fischer Verlag, Stuttgart, (Zentralblatt für Bakteriologie Parasitenkunde, InfektionsKrankheiten und Hygiene, Supplement 10). pp 543–545
    [Google Scholar]
  43. Wood D. O., Carter M. J., Best G. K. 1977; Plasmid-mediated resistance to gentamicin in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 12:513–517
    [Google Scholar]
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