1887

Abstract

Summary

A family of small plasmids encoding resistance to nucleic acid-binding (NAB) compounds has recently been identified in strains of isolated in Italy, Texas and Western Australia. The mol. wts of the NAB-resistance plasmids are in the range (1.5–1.9) × 10 and all but one encode resistance to acridine yellow, ethidium bromide and quaternary ammonium compounds. The largest of the plasmids, pWG1773, differed in that it did not confer resistance to ethidium bromide. Restriction enzyme analysis of these plasmids revealed four distinct patterns corresponding to plasmids of four different mol. wts and physical maps were constructed based on the restriction patterns. Two plasmid types of molecular sizes approximately 2440 and 2240 base pairs had a 610-base pair region in common. Physical maps of the other two plasmid types were not related. The presence of a family of small NAB-resistance plasmids which carry no other known phenotypic markers provides further evidence for the strong selective advantage associated with maintenance of this determinant in clinical isolates of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-1-9
1986-08-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/1/medmicro-22-1-9.html?itemId=/content/journal/jmm/10.1099/00222615-22-1-9&mimeType=html&fmt=ahah

References

  1. Archer G. L., Johnston J. L. 1983; Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrobial Agents and Chemotherapy 24:70–77
    [Google Scholar]
  2. Daniels D. L., Schroeder J. L., Szybalski W., Sanger F., Blattner F. R. 1983 A molecular map of Coliphage Lambda In Hendrix R. W. (eds) Lambda II Cold Spring Harbour Laboratory NY; pp 469–517
    [Google Scholar]
  3. Duggleby R. G., Kinns H., Rood J. I. 1981; A computer program for determining the size of DNA restriction fragments. Analytical Biochemistry 110:49–55
    [Google Scholar]
  4. Emslie K. R., Townsend D. E., Bolton S., Grubb W. B. 1985a; Two distinct resistance determinants to nucleic acid-binding compounds in Staphylococcus aureus?. FEMS Microbiology Letters 27:61–64
    [Google Scholar]
  5. Emslie K. R., Townsend D. E., Grubb W. B. 1985b; A resistance determinant to nucleic acid-binding compounds in methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 20:139–145
    [Google Scholar]
  6. Ericson C. 1969; Resistance to acriflavine and cadmium and changed phage reactions: markers of a new staphylococcal penicillinase plasmid?. Acta Pathologica et Microbiologica Scandinavica 76:333
    [Google Scholar]
  7. Grubb W. B., Townsend D. E., Greed L. C., Ashdown N., Momoh M. 1983; Characteristics of methicillin-resistant Staphylococcus aureus endemic in Australian hospitals. In Spitzy K. H., Karrer K. (eds) Proceedings of the 13th International Congress of Chemotherapy Verlag H. Egermann; Vienna: Part 83:23–26
    [Google Scholar]
  8. Johnston L. H., Dyke K. G. H. 1969; Ethidium bromide resistance, a new marker on the staphylococcal penicillinase plasmid. Journal of Bacteriology 100:1413–1414
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular cloning. A laboratory manual Cold Spring Harbour Laboratory, NY; pp 493–503
    [Google Scholar]
  10. Novick R. P., Bouanchaud D. 1971; Extrachromosomal nature of drug resistance in Staphylococcus aureus. Annals of the New York Academy of Science 182:279–294
    [Google Scholar]
  11. Townsend D. E., Ashdown N., Bolton S., Grubb W. B. 1985a; The use of cetyltrimethylammonium bromide for the rapid isolation from Staphylococcus aureus of relaxable and non-relaxable plasmid DNA suitable for in vitro manipulation. Letters in Applied Microbiology 1:87–94
    [Google Scholar]
  12. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984a; Analysis of plasmids mediating gentamicin resistance in methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 13:347–352
    [Google Scholar]
  13. Townsend D. E., Ashdown N., Greed L. C., Grubb W. B. 1984b; Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. Journal of Antimicrobial Chemotherapy 14:115–124
    [Google Scholar]
  14. Townsend D. E., Ashdown D., Grubb W. B. 1985b; Evolution of Australian isolates of methicillin-resistant Staphylococcus aureus—a problem of plasmid incompatibility?. Journal of Medical Microbiology 20:49–61
    [Google Scholar]
  15. Townsend D. E., Ashdown N., Momoh M., Grubb W. B. 1985c; Distribution of plasmid-borne resistance to nucleic acid binding compounds in methicillin-resistant Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 15:417–434
    [Google Scholar]
  16. Townsend D. E., Greed L. C., Ashdown N., Grubb W. B. 1983a; Plasmid-mediated resistance to quaternary ammonium compounds in methicillin-resistant Staphylococcus aureus. Medical Journal of Australia 2:310
    [Google Scholar]
  17. Townsend D. E., Grubb W. B., Ashdown N. 1983b; Gentamicin-resistance in methicillin-resistant Staphylococcus aureus. Pathology 15:169–174
    [Google Scholar]
  18. Townsend D. E., Grubb W. B., Ashdown N. 1983c; Genetics of drug resistance in methicillin-resistant Staphylococcus aureus from Australian Hospitals. Journal of Hospital Infection 4:331–337
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-1-9
Loading
/content/journal/jmm/10.1099/00222615-22-1-9
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error