1887

Abstract

Summary

One hundred and forty seven isolates of were collected from diverse clinical and environmental sources in south-east Texas. Natural isolates were compared with hospital strains for the occurrence of 12 potential virulence determinants. Their overall frequency was as follows: haemolytic activity 48%; lecithinase 95%; lipase 95%; motility 99%; pigmentation 24%; plasmid carriage 46%; proteolytic activity 98%; siderophore activity 99%; urease activity 5%; mannosesensitive haemagglutination 96%; mannose-resistant haemagglutination 61%; and mannose-resistant type-K haemagglutination (MR/K-HA) 68%. Clinical strains demonstrated a significantly higher occurrence of MR/K-HA (p<0.001) and nonpigmentation (p<0.01) than environmental isolates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-2-151
1986-09-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/2/medmicro-22-2-151.html?itemId=/content/journal/jmm/10.1099/00222615-22-2-151&mimeType=html&fmt=ahah

References

  1. Adegbola R. A., Old D. C. 1982; New fimbrial hemagglutinin in Serratia species. Infection and Immunity 38:306–315
    [Google Scholar]
  2. Ball A. P., McGhie D., Geddes A. M. 1977; Serratia marcescens in a general hospital. Quarterly Journal of Medicine New Series 46:63–71
    [Google Scholar]
  3. Boyden S. V. 1951; The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. Journal of Experimental Medicine 93:107–120
    [Google Scholar]
  4. Cooper R. L., Constable I. J. 1977; Infective keratitis in soft contact lens wearers. British Journal of Ophthamology 61:250–254
    [Google Scholar]
  5. Cox C. D., Adams P. 1985; Siderophore activity of pyoverdin for Pseudomonas aeruginosa . Infection and Immunity 48: HOBS
    [Google Scholar]
  6. Curtiss R. 1965; Chromosomal aberrations associated with mutations to bacteriophage resistance in Escherichia coli . Journal of Bacteriology 89:28–40
    [Google Scholar]
  7. Dauenhauer S. A., Hull R. A., Williams R. P. 1984; Cloning and expression in Escherichia coli of Serratia marcescens genes encoding prodigiosin biosynthesis. Journal of Bacteriology 158:1128–1132
    [Google Scholar]
  8. Davis J. T., Foltz E., Blackemore W. S. 1970; Serratia marcescens′. A pathogen of increasing clinical importance. Journal of the American Medical Association 214:2190–2192
    [Google Scholar]
  9. Duguid J. P. 1959; Fimbriae and adhesive properties in Klebsiella strains. Journal of General Microbiology 21:271–286
    [Google Scholar]
  10. Duguid J. P., Old D. C. 1980; Adhesive properties of Enterobacteriaceae. In Beachey E. H. (ed) Bacterial adherence (Receptors and recognition, series, B. vol 6.) Chapman and Hall; London: pp 185–217
    [Google Scholar]
  11. Duncan C. L. 1975; Role of clostridial toxins in pathogenesis. In Schlessinger D. (ed) Microbiology—1975 American Society for Microbiology; Washington D.C: pp 283–291
    [Google Scholar]
  12. Farmer J. J. 1976; Detection of Serratia outbreaks in hospital. Lancet 2:455–459
    [Google Scholar]
  13. Fulton M., Forney C. E., Leifson E. 1959; Identification of Serratia occurring in man and animals. Canadian Journal of Microbiology 5:269–275
    [Google Scholar]
  14. Grimont Grimont F. 1978; Biotyping of Serratia marcescens and its use in epidemiological studies. Journal of Clinical Microbiology 8:73–83
    [Google Scholar]
  15. Grimont Grimont F., DulongdeRosnay H. L. C., Sneath P. H. A. 1977; Taxonomy of the genus Serratia . Journal of General Microbiology 98:39–66
    [Google Scholar]
  16. Hagberg Hull R., Hull S., Falkow S., Freter R., SvanborgEden C. 1983; Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infection and Immunity 40:265–272
    [Google Scholar]
  17. Hedges R. W., Datta N. 1975; R Factors from Serratia marcescens . Journal of General Microbiology 86:88–92
    [Google Scholar]
  18. Johnson E., Ellner P. D. 1974; Distribution of Serratia species in clinical specimens. Applied Microbiology 28:513–514
    [Google Scholar]
  19. Lankford C. E., Eklund C. E. 1966; Laboratory manual for public health bacteriology. University of Texas; Austin: pp 174–175
    [Google Scholar]
  20. Lass J. H., Haaf J., Foster C. S., Belcher C. 1981; Visual outcome in eight cases of Serratia marcescens keratitis. American Journal of Ophthalmology 92:384–390
    [Google Scholar]
  21. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1:190–206
    [Google Scholar]
  22. L’Eplattenier F., Murase I., Martell A. E. 1967; New multidentate ligands. VI. Chelating tendencies of N.N′-di(2-hydroxybenzyl) ethylene-diamine-N,N′-diacetic acid. Journal of the American Chemical Society 89:837–843
    [Google Scholar]
  23. Lyerly D. M., Kreger A. S. 1983; Importance of Serratia protease in the pathogenesis of experimental Serratia marcescens pneumonia. Infection and Immunity 40:113–119
    [Google Scholar]
  24. McEntegart M. G., Porterfield J. S. 1949; Bacteraemia following dental extractions. Lancet 2:596–598
    [Google Scholar]
  25. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1537
    [Google Scholar]
  26. O’Brien T. F., Ross D. G., Guzman M. A., Medeiros A. A., Hedges R. W., Botstein D. 1980; Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrobial Agents and Chemotherapy 17:537–543
    [Google Scholar]
  27. Old D. C., Adegbola R., Scott S. S. 1983; Multiple fimbrial hemagglutinins in Serratia species. Medical Microbiology and Immunology 172:107–115
    [Google Scholar]
  28. Parsons C. L., Stauffer C., Mulholland S. G., Griffith D. P. 1984; Effect of ammonium on bacterial adherence to bladder transitional epithelium. Journal of Urology 132:365–366
    [Google Scholar]
  29. Pazin G. J., Braude A. I. 1974; Immobilizing antibodies in urine. II. Prevention of ascending spread of Proteus mirabilus . Investigative Urology 12:129–133
    [Google Scholar]
  30. Portnoy D. A., Moseley S. L., Falkow S. 1981; Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infection and Immunity 31:775–782
    [Google Scholar]
  31. Rogers H. J. 1973; Iron-binding catechols and virulence in Escherichia coli . Infection and Immunity 7:445–456
    [Google Scholar]
  32. Rubens C. E., McGee Z. A., Farrar W. E. 1980; Loss of an aminoglycoside resistance plasmid by Serratia marcescens during treatment of meningitis with amikacin. Journal of Infectious Diseases 141:346–350
    [Google Scholar]
  33. Smibert R. M., Krieg N. R. 1981; General characterization. In Gerhardt P. (ed) Manual of methods for general bacteriology American Society for Microbiology; Washington D.C: pp 409–443
    [Google Scholar]
  34. Smith H. W., Huggins M. B. 1976; Further observations on the associations of the Colicine V plasmid of Escherichia coli with pathogenicity and with survival in the alimentary tract. Journal of General Microbiology 92:335–350
    [Google Scholar]
  35. Sokol P. A., Ohman D. E., Iglewski B. H. 1979; A more sensitive plate assay for detection of protease production by Pseudomonas aeruginosa . Journal of Clinical Microbiology 9:538–540
    [Google Scholar]
  36. Starr M. P., Grimont F., Starr P. B. 1976; Caprylate-thallous agar medium for selectively isolating Serratia and its utility in the clinical laboratory. Journal of Clinical Microbiology 4:270–276
    [Google Scholar]
  37. Tabaqchali S., Chambers T. J., Brooks H. J. L. 1977; Serratia marcescens in hospital practice. Lancet 1:306–307
    [Google Scholar]
  38. Welch R. A., Dellinger E. P., Minshew B., Falkow S. 1981; Haemolysin contributes to virulence of extra-intestinal E. coli infections. Nature 294:665–667
    [Google Scholar]
  39. Wilfert J. N., Barrett F. F., Ewing W. H., Finland M., Kass E. H. 1970; Serratia marcescens: biochemical, serological & epidemiological characteristics and antibiotic susceptibility of strains isolated at Boston City Hospital. Applied Microbiology 19:345–352
    [Google Scholar]
  40. Wilkowske C. J., Washington J. A., Martin W. J., Ritts R. E. 1970; Serratia marcescens: biochemical characteristics, antibiotic susceptibility patterns, and clinical significance. Journal of the American Medical Association 214:2157–2162
    [Google Scholar]
  41. Willett H. P. 1980; Staphylococcus. In Joklik W. K., Willett H. P., Amos D. B. (eds) Zinser Microbiology 17th edn Appleton Century-Crofts; New York: pp 532–552
    [Google Scholar]
  42. Williams P. H., Warner P. J. 1980; ColV plasmid-mediated, colicin V-independent iron uptake system of invasive strains of Escherichia coli . Infection and Immunity 29:411–416
    [Google Scholar]
  43. Woodward Clarke K. B. 1913; A case of infection in man by the bacterium prodigiosum. Lancet 1:314–315
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-2-151
Loading
/content/journal/jmm/10.1099/00222615-22-2-151
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error