f Cytotoxic activity in broth-culture filtrates of Campylobacter pylori
- Authors: R. D. Leunk*, P. T. Johnson, B. C. David, W. G. Kraft, D. R. Morgan†
- First Published Online: 01 June 1988, Journal of Medical Microbiology 26: 93-99, doi: 10.1099/00222615-26-2-93
- Subject: Articles
- Issue Published:
Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70°C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 x 103. It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.
Present address: Miami Valley Laboratories, The Procter and Gamble Co., Cincinnatti, OH, USA.
Address for correspondence: Dr D. R. Morgan, PO Box 398707, Cincinnatti, OH 45239–8707, USA.
© 1988 The Pathological Society of Great Britain and Ireland | Published by the Microbiology Society
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