1887

Abstract

SUMMARY

Strains of fusobacteria (143) were examined by pyrolysis mass spectrometry (Py-MS) with a Horizon Instruments PYMS 200X. and were discriminated. Strains of fusobacteria isolated from tropical ulcers, although similar to in conventional tests, were discriminated from each of these species in Py-MS. Identification of 416 spectra to species level agreed with conventional bacteriological methods in 91.8% of cases, was equivocal in 3.4% and disagreed in 4.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-28-3-227
1989-03-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/28/3/medmicro-28-3-227.html?itemId=/content/journal/jmm/10.1099/00222615-28-3-227&mimeType=html&fmt=ahah

References

  1. Adriaans B., Drasar B. S. 1987; The isolation of fusobacteria from tropical ulcers. Epidemiology and Infection 99:361–372
    [Google Scholar]
  2. Aries R. E., Gutteridge C. S., Ottley T. W. 1986; Evaluation of a low-cost, automated pyrolysis-mass spectrometer. Journal of Analytical and Applied Pyrolysis 9:81–98
    [Google Scholar]
  3. Bennett K. W., Duerden B. I. 1985; Identification of fusobacteria in a routine diagnostic laboratory. Journal of Applied Bacteriology 59:171–181
    [Google Scholar]
  4. Chandran G., Schenfield L. 1985; Osteomyelitis of the femur due to Fusobacterium mortiferum. Southern Medical Journal 78:365
    [Google Scholar]
  5. Drucker D. B. 1981; Microbiological applications of gas chromatography. Cambridge University Press; Cambridge:
    [Google Scholar]
  6. Eshuis W., Kistemaker P. G., Meuzelaar H. L. C. 1977; Some numerical aspects of reproducibility and specificity. In Jones C. E. R., Cramers C. A. (eds) Analytical pyrolysis Elsevier; Amsterdam: pp 151–156
    [Google Scholar]
  7. Finegold S. M. 1977; Anaerobic bacteria in human disease. Academic Press; New York:
    [Google Scholar]
  8. George W. L., Kirby B. D., Sutter V. L., Citron D. M., Finegold S. M. 1981; Gram-negative anaerobic bacilli: their role in infection and patterns of susceptibility to antimicrobial agents. II. Little-known Fusobacterium species and miscellaneous genera. Reviews of Infectious Diseases 3:599–626
    [Google Scholar]
  9. Gharbia S. E., Shah H. N. 1988; Characteristics of glutamate dehydrogenase, a new diagnostic marker for the genus Fusobacterium. Journal of General Microbiology 134:327–332
    [Google Scholar]
  10. Gutteridge C. S., Norris J. R. 1979; The application of pyrolysis techniques to the identification of micro-organisms. Journal of Applied Bacteriology 47:5–43
    [Google Scholar]
  11. Gutteridge C. S., Vallis L., MacFie H. J. H. 1985; Numerical methods in the classification of micro-organisms by pyrolysis mass spectrometry. In Goodfellow M., Jones D., Priest F. G. (eds) Computer assisted bacterial systematics Academic Press; London: pp 369–401
    [Google Scholar]
  12. Hall B. B., Fitzgerald R. H., Rosenblatt J. E. 1983; Anaerobic osteomyelitis. Journal of Bone and Joint Surgery 65-A:30–35
    [Google Scholar]
  13. Hindmarch J. M., Magee J. T. 1987; The staphylococci: a classification and identification study using pyrolysis gas-liquid chromatography. Journal of Analytical and Applied Pyrolysis 11:527–538
    [Google Scholar]
  14. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobic Laboratory Manual. 4th edn Anaerobe Laboratory Virginia Polytechnic Institute and State University, Blacksburg, VA; USA:
    [Google Scholar]
  15. Holdeman L. V., Kelley R. W., Moore W. E. C. 1984; Bacteroidaceae. In Krieg N. R. (ed) Bergey’s Manual of systematic bacteriology vol 1 Williams and Wilkins Co; Baltimore, MD: pp 602–662
    [Google Scholar]
  16. HuffS M., Meuzelaar H. L. C., Pope D. P. L., Kjeldsberg C. R. 1981; Characterization of leukemic and normal white blood cells by Curie-point pyrolysis-mass spectrometry. I. Numerical evaluation of the results of a pilot-study. Journal ofAnalytical and Applied Pyrolysis 3:95–109
    [Google Scholar]
  17. Lemierre A. 1936; On certain septicaemias due to anaerobic organisms. Lancet 1:701–703
    [Google Scholar]
  18. Love D. N., Cato E. P., Johnson A. L., Jones R. F., Bailey M. 1987; Deoxyribonucleic acid hybridisation among strains of fusobacterium isolated from soft tissue infections of cats: comparison with human and animal type strains from oral and other sites. International Journal of Systematic Bacteriology 37:23–26
    [Google Scholar]
  19. Magee J. T., Hindmarch J. M., Meechan D. F. 1983; Identification of staphylococci by pyrolysis gas-liquid chromatography. Journal of Medical Microbiology 16:483–495
    [Google Scholar]
  20. Meuzelaar H. L. C., Kistemaker P. G. 1973; A technique for fast and reproducible fingerprinting of bacteria by pyrolysis mass spectrometry. Analytical Chemistry 45:587–590
    [Google Scholar]
  21. Mitre R. J., Rotherham E. B. 1974; Anaerobic septicemia from thrombophlebitis of the internal jugular vein. Successful treatment with metromidazole. Journal of the American Medical Association 230:1168–1169
    [Google Scholar]
  22. Moore-Gillon J., Lee T. H., Eykyn S., Phillips I. 1984; Necrobacil-losis: a forgotten disease. British Medical Journal 288:1526–1527
    [Google Scholar]
  23. Norris J. R. 1980; Introduction. In Goodfellow M., Board R. G. (eds) Microbiological classification and identification Academic Press; London: pp 1–10
    [Google Scholar]
  24. O’Grady L. R., Ralph E. D. 1976; Anaerobic meningitis and bacteremia caused by Fusobacterium species. American Journal of Diseases of Childhood 130:871–873
    [Google Scholar]
  25. Ottley T. W., Maddock J. 1986; The use of pyrolysis mass spectrometry. Laboratory Practice October 53–55
    [Google Scholar]
  26. Reig M., Baquero F., Garcia-Campello M., Loza E. 1985; Leptotrichia buccalis bacteremia in neutropenic children. Journal of Clinical Microbiology 22:320–321
    [Google Scholar]
  27. Seidenfeld S. M., Sutker W. L., Luby J. P. 1982; Fusobacterium necrophorum septicemia following oropharyngeal infection. Journal of the American Medical Association 248:1348–1350
    [Google Scholar]
  28. Shute L. A., Berkeley R. C. W., Norris J. R., Gutteridge C. S. 1985; Pyrolysis mass spectrometry in bacterial systematics. In Goodfellow M., Minnikin D. E. (eds) Chemical methods in bacterial systematics Academic Press; London: pp 95–114 SPSSX User’s Guide (1983 McGraw-Hill Book Company, New York
    [Google Scholar]
  29. Templeton W. C., Wawrukiewicz A., Melo J. C., Schiller M. G., Raff M. J. 1983; Anaerobic osteomyelitis of long bones. Reviews of Infectious Disease 5:692–711
    [Google Scholar]
  30. Walker C. B., Ratcliff D., Muller D., Mandell R., Socransky S. S. 1979; Medium for selective isolation of Fusobacterium nucleatum from human periodontal pockets. Journal of Clinical Microbiology 10:844–849
    [Google Scholar]
  31. Ward J. H. 1963; Hierarchical grouping to optimise an objective function. Journal of the American Statistical Association 58:236–244
    [Google Scholar]
  32. Wishart D. 1982; Clustan 2 user manual. Edinburgh University Press; Edinburgh:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-28-3-227
Loading
/content/journal/jmm/10.1099/00222615-28-3-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error