1887

Abstract

Summary

Strains of of serotype Sendai, producing a mannose-resistant and eluting haemagglutinin (MREHA) when cultured at 37°C but not at 18°C, were examined by electronmicroscopy after negative staining. Production of this MREHA, previously thought to be nonfimbrial, was correlated with the presence of thick fimbriae with an external diameter of 13.6nm. These fimbriae were readily fragmented and, when purified, had an estimated M of 28 Kda. Production of fimbrial MREHA by Sendai strains was associated with the ability to adhere to a wide range of substrates and to form a fimbrial pellicle at the surface of liquid media incubated statically in air. The origin of this unusual Sendai fimbrial MREHA is unknown. Thin filamentous structures produced independently of fimbrial MREHA by Sendai strains were also described. Fimbrial MREHA was not produced by strains of the antigenically similar serotype Miami which, however, and unlike Sendai strains, formed mannose-sensitive haemagglutinin and type-1 fimbriae. The ability to differentiate strains of Miami and Sendai (serotype , 9, 12:a:1, 5) by means of their fimbriae is noted.

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1989-09-01
2024-03-29
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References

  1. Adegbola R. A., Old D. C. 1982; New fimbrial hemagglutinin in Serratia species. Infection and Immunity 38:306–315
    [Google Scholar]
  2. Adegbola R. A., Old D. C., Aleksic S. 1983; Rare MR/K-like haemagglutinins (and type 3-like fimbriae) of Salmonella strains. FEMS Microbiology Letters 19:233–238
    [Google Scholar]
  3. Aleksić S., Rohde R., Aleksic V. 1978; Serologische Verwandt-schaft der Fimbrienantigene bei Salmonella Subgenus I– Yersinia enterocolitica einerseits und Salmonella Subgenus II–Arizona (= Salmonella Subgenus III) andererseits. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektion-skrankheiten und Hygiene I. Originale A 241:418–426
    [Google Scholar]
  4. Aoki K., Sakai K. 1925; Ueber von Hogcholerabazillen einerseits, von Paratyphus A-Bazillen andererseits schwer differen-zierbare, in Japan vorkommende Paratyphusbazillen. Centralblatt für Bakteriologie, Parasitenkunde und Infektionskrankheiten I. Originale A 95:152–157
    [Google Scholar]
  5. Burke D. A., Axon A. T. R. 1987; Ulcerative colitis and Escherichia coli with adhesive properties. Journal of Clinical Pathology 40:782–786
    [Google Scholar]
  6. de Graaf F. K., Roorda I. 1982; Production, purification and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M. Infection and Immunity 36:751–758
    [Google Scholar]
  7. Duchet-Suchaux M., Bertin A., Dubray G. 1988; Morphological description of surface structures on strain B41 of bovine enterotoxigenic Escherichia coli bearing both K99 and F41 antigens. Journal of General Microbiology 134:983–995
    [Google Scholar]
  8. Duguid J. P. 1985; Antigens of type-1 fimbriae. In Stewart-Tull D. E. S., Davies M. (eds) Immunology of the bacterial cell envelope Wiley; Chichester: pp 301–318
    [Google Scholar]
  9. Duguid J. P., Campbell I. 1969; Antigens of the type-1 fimbriae of salmonellae and other enterobacteria. Journal of Medical Microbiology 2:535–553
    [Google Scholar]
  10. Duguid J. P., Old D. C. 1980; Adhesive properties of Enterobacteriaceae. In Beachey E. H. (ed) Bacterial adherence (Receptors and recognition series B. vol 6 Chapman and Hall; London: pp 185–217
    [Google Scholar]
  11. Duguid J. P., Anderson E. S., Campbell I. 1966; Fimbriae and adhesive properties in salmonellae. Journal of Pathology and Bacteriology 92:107–138
    [Google Scholar]
  12. Duguid J. P., Clegg S., Wilson M. I. 1979; The fimbrial and non-fimbrial haemagglutinins of Escherichia coli. Journal of Medical Microbiology 12:213–227
    [Google Scholar]
  13. Duguid J. P., Smith I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages (“fimbriae”) and haemagglutinating activity in Bacterium coli. Journal of Pathology and Bacteriology 70:335–348
    [Google Scholar]
  14. Ewing W. H. 1985; Edwards and Ewing’s Identification of Enterobacteriaceae. 4th edn Elsevier; New York:
    [Google Scholar]
  15. Firon N., Ofek I., Sharon N. 1983; Carbohydrate specificity of the surface lectins of Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium. Carbohydrate Research 120:235–249
    [Google Scholar]
  16. Halula M. C., Stocker B. A. D. 1987; Distribution and properties of the mannose-resistant hemagglutinin produced by Salmonella species. Microbial Pathogenesis 3:455–459
    [Google Scholar]
  17. Jones G. W., Richardson L. A. 1981; The attachment to, and invasion of, HeLa cells by Salmonella typhimurium: the contribution of mannose-sensitive and mannose-resistant haemagglutinating activities. Journal of General Microbiology M1:361–370
    [Google Scholar]
  18. Korhonen T. K., Lounatmaa K., Ranta H., Kuusi N. 1980; Characterization of type-1 pili of Salmonella typhimurium LT2. Journal of Bacteriology 144:800–805
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  20. Le Minor L. 1988; Typing of Salmonella species. European Journal of Clinical Microbiology and Infectious Diseases 7:214–218
    [Google Scholar]
  21. Le Minor L., Popoff M. Y. 1987; Designation of Salmonella enterica sp. nov., nom. rev., as the type and only species of the genus Salmonella. International Journal of Systematic Bacteriology 37:465–468
    [Google Scholar]
  22. Le Minor L., Popoff M. Y. 1988; Antigenic formulas of the Salmonella serovars, 5th revision. WHO Collaborating Centre for Reference and Research on Salmonella Institut Pasteur; Paris, France:
    [Google Scholar]
  23. Le Minor L., Le Minor S., Grimont P. A. D. 1985; Rapport quadriennal du Centre national des Salmonella sur l’origine et la répartition en sérotypes des souches isolees en France continentale au cours des anées 1980a 1983. Revue d’ Epidemiologie et de Sante Publique 33:13–21
    [Google Scholar]
  24. Lindquist B. L., Lebenthal E., Lee P.-C., Stinson M. W., Merrick J. M. 1987; Adherence of Salmonella typhimurium to small-intestinal enterocytes of the rat. Infection and Immunity 55:3044–3050
    [Google Scholar]
  25. MacNab R. M. 1987; Flagella. In Neidhardt F. C. (ed) Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology vol 1 American Society for Microbiology; Washington, D.C.: pp 70–83
    [Google Scholar]
  26. Manning E. J., Baird G. D., Jones P. W. 1986; The role of plasmid genes in the pathogenicity of Salmonella dublin. Journal of Medical Microbiology 21:239–243
    [Google Scholar]
  27. Morris J. A., Thorns C., Scott A. C., Sojka W. J., Wells G. A. 1982; Adhesion in vitro and in vivo associated with an adhesive antigen (F41) produced by a K99 mutant of the reference strain Escherichia coli B41. Infection and Immunity 36:1146–1153
    [Google Scholar]
  28. Old D. C. 1972; Inhibition of the interaction between fimbrial haemagglutinins and erythrocytes by D-mannose and other carbohydrates. Journal of General Microbiology 71:149–157
    [Google Scholar]
  29. Old D. C. 1985; Haemagglutination methods in the study of Escherichia coli. In Sussman M. (ed) The virulence of Escherichia coli: reviews and methods Academic Press; London: pp 287–313
    [Google Scholar]
  30. Old D. C., Adegbola R. A. 1985; Antigenic relationships among type-3 fimbriae of Enterobacteriaceae revealed by immunoelectronmicroscopy. Journal of Medical Microbiology 20:113–121
    [Google Scholar]
  31. Old D. C., Duguid J. P. 1970; Selective outgrowth of fimbriate bacteria in static liquid medium. Journal of Bacteriology 103:447–456
    [Google Scholar]
  32. Old D. C., Duguid J. P. 1971; Selection of fimbriate transductants of Salmonella typhimurium dependent on motility. Journal of Bacteriology 107:655–658
    [Google Scholar]
  33. Old D. C., Tavendale A. 1986; Adhesion of Salmonella dublin to HEp2 epithelial cells. Letters in Applied Microbiology 3:73–76
    [Google Scholar]
  34. Old D. C., Corneil I., Gibson L. F., Thomson A. D., Duguid J. P. 1968; Fimbriation, pellicle formation and the amount of growth of salmonellas in broth. Journal of General Microbiology 51:1–16
    [Google Scholar]
  35. Old D. C., Klaassen B., Crichton P. B. 1985; A novel, non-fimbrial mannose-sensitive and eluting (MSE) haemagglutinin of Yersinia frederiksenii strains. FEMS Microbiology Letters 28:81–84
    [Google Scholar]
  36. Old D. C., Yakubu D. E., Crichton P. B. 1987; Demonstration by immuno-electronmicroscopy of antigenic heterogeneity among P fimbriae of strains of Escherichia coli. Journal of Medical Microbiology 23:247–253
    [Google Scholar]
  37. Rohde R., Aleksic S., Muller G., Plavsic S., Aleksic V. 1975; Profuse fimbriae conferring O-inagglutinability to several strains of S. typhimurium and 5. enteritidis isolated from pasta products. Cultural, morphological and serological experiments. Zentralblatt fur Bakteriologie, Parasitenkunde, Infek-tionskrankheiten und Hygiene I. Originate A 230:38–50
    [Google Scholar]
  38. Rozgonyi F., Szitha K. R., Ljungh A., Baloda S. B., Hjerten S., Wadstrom T. 1985; Improvement of the salt aggregation test to study bacterial cell-surface hydrophobicity. FEMS Microbiology Letters 30:131–138
    [Google Scholar]
  39. Sato H., Okinaga K. 1987; Role of pili in the adherence of Pseudomonas aeruginosa to mouse epidermal cells. Infection and Immunity 55:1774–1778
    [Google Scholar]
  40. Scott S. S., Old D. C. 1981; Mannose-resistant and eluting (MRE) haemagglutinins, fimbriae and surface structure in strains of Haemophilus. FEMS Microbiology Letters 10:235–240
    [Google Scholar]
  41. Tavendale A., Jardine C. K. H., Old D. C., Duguid J. P. 1983; Haemagglutinins and adhesion of Salmonella typhimurium to HEp2 and HeLa cells. Journal of Medical Microbiology 16:371–380
    [Google Scholar]
  42. Yakubu D. E., Senior B. W., Old D. C. 1989; A novel fimbrial haemagglutinin produced by a strain of Salmonella of serotype Salinatis. FEMS Microbiology Letters 57:29–34
    [Google Scholar]
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