1887

Abstract

Summary

The mode of action of haemolysin (VVH) on mast cells from the peritoneal cavity of the rat was examined. VVH induced histamine release, and damage to the mast cells, in a dose-dependent fashion. When 1 μg of VVH was added to . 10 mast cells at 37°C, histamine release was observed after a lag period of 5-10 s, and was complete within 5 min. The action was temperature-dependent, and was not induced at 4°C. Disodium cromoglycate, a membrane stabiliser for mast cells, inhibited the histamine release significantly, but the effect was not dose-dependent. Moreover, leakage of lactate dehydrogenase from VVH-treated mast cells was observed. These results suggest that VVH acts on the cell membrane of mast cells and is cytolytic.

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1990-05-01
2024-03-28
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References

  1. Tison D L, Kelly M T. Vibrio species of medical importance. Diagn Microbiol Infect Dis 1984; 2:263–276
    [Google Scholar]
  2. Janda J M, Powers C, Bryant R G, Abbott S L. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev 1988; 1:245–267
    [Google Scholar]
  3. Klontz K C, Lieb S, Schreiber M, Janowski H T, Baldy L M, Gunn R A. Syndromes of Vibrio vulnificus infections. Clinical and epidemiological features in Florida cases 1981—1982. Am Intern Med 1988; 109:318–323
    [Google Scholar]
  4. Blake P A, Weaver R E, Hollis D G. Diseases of humans (other than cholera) caused by vibrios. Ann Rev Microbiol 1980; 34:341–367
    [Google Scholar]
  5. Tacket C O, Brenner F, Blake P A. Clinical features and an epidemiological study of Vibrio vulnificus infections. J Infect Dis 1984; 149:558–561
    [Google Scholar]
  6. Shirouzu K, Miyamoto Y, Yasaka T, Matsubayashi Y, Morimatsu M. Vibrio vulnificus septicaemia. Acta Pathol Jpn 1985; 35:731–739
    [Google Scholar]
  7. Ito H, Kaetsu M, Saeki Y et al. A saved case of Vibrio vulnificus infection with shock, DIC and necrotizing fasciitis. Kansenshogaku Zasshi 1986; 60:695–700 (in Japanese)
    [Google Scholar]
  8. Morris J G. Vibrio vulnificus—a new monster of the deep?. Ann Intern Med 1988; 109:261–263
    [Google Scholar]
  9. Baethge B A, West B C. Vibrio vulnificus: did Hippocrates describe a fatal case?. Rev Infect Dis 1988; 10:614–615
    [Google Scholar]
  10. Tyring S K, Lee P C. Hemorrhagic bullae associated with Vibrio vulnificus septicemia. Report of two cases. Arch Dermatol 1986; 122:818–820
    [Google Scholar]
  11. Chin K P, Lowe M A, Tong M J, Koehler A L. Vibrio vulnificus infection after raw oyster ingestion in a patient with liver disease and acquired immune deficiency syndrome-related complex. Gastroenterol 1987; 92:796–799
    [Google Scholar]
  12. Miyoshi S, Shinoda S. Role of the protease in the permeability enhancement by Vibrio vulnificus. Microbiol Immunol 1988; 32:1025–1032
    [Google Scholar]
  13. Miyoshi N, Shimizu C, Miyoshi S, Shinoda S. Purification and characterization of Vibrio vulnificus protease. Microbiol Immunol 1987; 31:13–25
    [Google Scholar]
  14. Miyoshi S, Sugiyama K, Suzuki Y, Furuta H, Miyoshi N, Shinoda S. Enhancement of vascular permeability due to histamine-releasing effect of Vibrio vulnificus protease in rat skin. FEMS Microbiol Lett 1987; 40:95–98
    [Google Scholar]
  15. Miyoshi N, Miyoshi S, Sugiyama K, Suzuki Y, Furuta H, Shinoda S. Activation of the plasma kallikrein-kinin system by Vibrio vulnificus protease. Infect Immun 1987; 55:1936–1939
    [Google Scholar]
  16. Kreger A, Lockwood D. Detection of extracellular toxin(s) produced by Vibrio vulnificus. Infect Immun 1981; 33:583–590
    [Google Scholar]
  17. Gray L D, Kreger A S. Mouse skin damage caused by cytolysin from Vibrio vulnificus and by V. vulnificus infection. J Infect Dis 1987; 155:236–241
    [Google Scholar]
  18. Yamanaka H, Satoh T, Katsu T, Shinoda S. Mechanism of haemolysis by Vibrio vulnificus haemolysin. J Gen Microbiol 1987; 133:2859–2864
    [Google Scholar]
  19. Wells E, Mann J. Phosphorylation of a mast cell protein in response to treatment with anti-allergic compounds. Implications for the mode of action of sodium cromoglycate. Biochem Pharmacol 1983; 32:837–842
    [Google Scholar]
  20. Shore P A, Burkhalter A, Cohn V H. A method for the fluorometric assay of histamine in tissues. J Pharmacol Exp Ther 1959; 127:182–186
    [Google Scholar]
  21. Roy P D, Moran D M, Bryant V, Stevenson R, Stanworth D R. Further studies on histamine release from rat mast cells in vitro induced by peptides. Biochem J 1980; 191:233–237
    [Google Scholar]
  22. Sugiyama K, Furuta H. Histamine release induced by dynorphin-(l-13) from rat mast cells. Jpn J Pharmacol 1984; 35:247–252
    [Google Scholar]
  23. Orr T S, Hall D E, Gwilliam J M, Cox J S G. The effect of disodium cromoglycate on the release of histamine and degranulation of rat mast cells induced by compound 48—80. Life Sci 1971; 10:805–812
    [Google Scholar]
  24. Garland L G. Effect of cromoglycate on anaphylactic histamine release from rat peritoneal mast cells. Br J Pharmacol 1973; 49:128–130
    [Google Scholar]
  25. Spataro A C, Bosmann H B. Mechanism of action of disodium cromoglycate on mast cell calcium ion influx after a histamine-releasing stimulus. Biochem Pharmacol 1976; 25:505–510
    [Google Scholar]
  26. Lagunoff D, Martin T W, Read G. Agents that release histamine from mast cells. Amu Rev Pharmacol Toxicol 1983; 23:331–351
    [Google Scholar]
  27. Garland L G, Mongar J L. Inhibition by cromoglycate of histamine release from rat peritoneal mast cells induced by mixtures of dextran, phosphatidyl serine and calcium ions. Br J Pharmacol 1974; 50:137–143
    [Google Scholar]
  28. Carraway R, Cochrane D E, Lansman J B, Leeman S E, Paterson B M, Welch H J. Neurotensin stimulates exocytotic histamine secretion from rat mast cells and elevates plasma histamine levels. J Physiol (Lond) 1982; 323:403–414
    [Google Scholar]
  29. Stephen J, Pietrowski R A. Bacterial toxins, 2nd edn (Aspects of Microbiology 2). American Society for Microbiology Washington DC: 1986
    [Google Scholar]
  30. Suttorp N, Seeger W, Zucker-Reimann J, Roka L, Bhakdi S. Mechanism of leukotriene generation in polymorphonuclear leukocytes by staphylococcal alpha-toxin. Infect Immun 1987; 55:104–110
    [Google Scholar]
  31. Howell T W, Gomperts B D. Rat mast cells permeabilised with streptolysin O secrete histamine in response to Ca2+ at concentrations buffered in the micromolar range. Biochim Biophys Acta 1987; 927:177–183
    [Google Scholar]
  32. Scheffer J, Konig W, Braun V, Goebel W. Comparison of four hemolysin-producing organisms (Escherichia coli, Serratia marcescens, Aeromonas hydrophila and Listeria monocytogenes) for release of inflammatory mediaters from various cells. J Clin Microbiol 1988; 26:544–551
    [Google Scholar]
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