@article{mbs:/content/journal/jmm/10.1099/00222615-32-1-63, author = "Preston, N. W. and Zorgani, A. A. and Carter, E. J.", title = "Location of the Three Major Agglutinogens of Bordetella Pertussis by Immuno-Electronmicroscopy", journal= "Journal of Medical Microbiology", year = "1990", volume = "32", number = "1", pages = "63-68", doi = "https://doi.org/10.1099/00222615-32-1-63", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-32-1-63", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary When the three serotypes of Bordetella pertussis (types 1,2,3; 1,2 and 1,3) were labelled with agglutinins and protein-A gold, agglutinogen 1 was found on fimbriae and on the cell surface of types 1,2,3 and 1,2 but on the cell surface only of non-fimbriate type 1,3 organisms. In contrast, agglutinogen 2 was located on fimbriae only. Agglutinogen 3 was not labelled. When protein-A gold was replaced by immunoglobulin-G gold, agglutinogen 3 was found on the cell surface only, even of fimbriate bacteria of type 1,2,3. The implications of these findings for acellular vaccines are discussed.", }