f An improved selective medium for the isolation of Escherichia coli O 157
- Authors: P. A. Chapman, Christine A. Siddons, P. M. Zadik, Linda Jewes
- First Published Online: 01 August 1991, Journal of Medical Microbiology 35: 107-110, doi: 10.1099/00222615-35-2-107
- Subject: Articles
- Issue Published:
Summary. Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT+) Escherichia coli O157. However, many organisms other than VT+ E. coli O157, especially other serogroups of E. coli and Proteus spp., may not ferment sorbitol, and thus may be confused initially with VT+ E. coli O157. Rhamnose is not fermented by VT+ E. coli O157, but is by most sorbitol non-fermenting E. coli of other serogroups. Cefixime is a cephalosporin antibiotic that is more active against Proteus spp. than against E. coli. Inclusion of rhamnose and cefixime in sorbitol-MacConkey agar improves its selectivity for the isolation of VT+ E. coli O157.
© 1991 The Pathological Society of Great Britain and Ireland | Published by the Microbiology Society
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