1887

Abstract

. Representative strains of 22 serotypes of the fragilis group of and four non-fragilis control strains of , , and were tested by SDS-PAGE and immunoblotting with hyperimmune rabbit sera. SDS-PAGE showed 25 polypeptide bands but, after immunoblotting, 24 antigenic bands were observed in various combinations in all the strains. Three of these were detected only in the control strains, whereas six others were present in different combinations in all strains of the fragilis group but were not present in the controls. Cluster analysis of the antigenic bands showed that the controls were antigenically different from the fragilis group strains. Strains of the fragilis group from the same geographic localities grouped in single clusters; most faecal isolates and NCTC strains appeared separate. There was no correlation between the species of and their antigenic structure. SDS-PAGE with immunoblotting is a superior technique for typing the fragilis group of . Specific antigens have been identified which may be used in the serodiagnosis of infection with these organisms.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-35-2-118
1991-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/35/2/medmicro-35-2-118.html?itemId=/content/journal/jmm/10.1099/00222615-35-2-118&mimeType=html&fmt=ahah

References

  1. Moore WEC, Cato EP, Holdeman LV. Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. J Infect Dis 1969; 119:641–649
    [Google Scholar]
  2. Gorbach SL, Bartlett JG. Anaerobic infections. N Engl J Med 1974; 290:1177–1184
    [Google Scholar]
  3. McGowan K, Gorbach SL. Anaerobes in mixed infections. J Infect Dis 1981; 144:181–186
    [Google Scholar]
  4. Elhag KM, Bahar AM, Alwan MH, Mahajan KK, Sherif RA, Senthilselvan A. Anaerobes in surgical infections in Kuwait: a multicenter study on 379 patients. Med Principles Pract 1989; 1:44–51
    [Google Scholar]
  5. Lambe DW, Moroz DA. Serogrouping of Bacteroides fragilis subsp. fragilis by agglutination test. J Clin Microbiol 1976; 3:586–592
    [Google Scholar]
  6. Elhag KM, Bettelheim KA, Tabaqchali S. Serological studies of Bacteroides fragilis. J Hyg 1977; 79:233–241
    [Google Scholar]
  7. Babb JL, Cummins CS. Relationships between serological groups and deoxyribonucleic acid homology groups in Bacteroides fragilis and related species. J Clin Microbiol 1981; 13:369–379
    [Google Scholar]
  8. Elhag KM, Tabaqchali S. A study of the surface and somatic antigens of Bacteroides fragilis. J Hyg 1978; 80:439–449
    [Google Scholar]
  9. Elhag KM, Tabaqchali S. The distribution of Bacteroides fragilis serotypes amongst clinical strains. J Hyg 1978; 81:89–97
    [Google Scholar]
  10. Elhag KM, Senthilselvan A. A serogrouping scheme for the study of the epidemiology of Bacteroides fragilis. J Med Microbiol 1988; 27:199–205
    [Google Scholar]
  11. Ross CAC, Gilmore RF. Serological diagnosis of Bacteroides fragilis infections by a complement fixation test. J Clin Pathol 1978; 31:1083–1084
    [Google Scholar]
  12. Rissing JP, Buxton TB, Edmondson HT. Detection of specific IgG antibody in sera from patients infected with Bacteroides fragilis by enzyme-linked immunosorbent assay. J Infect Dis 1979; 140:994–998
    [Google Scholar]
  13. Okubadejo OA, Lightfoot NF, Hewitt WG. Diagnosis of Bacteroides fragilis infection with counter-immunoelectrophoresis. J Clin Pathol 1978; 31:1078–1082
    [Google Scholar]
  14. Paavonen J, Valtonen VV, Kasper DL, Malkamäki M, Mäkelä PH. Surgical evidence for the role of Bacteroides fragilis and Enterobacteriaceae in the pathogenesis of acute pelvic inflammatory disease. Lancet 1981; 1:293–295
    [Google Scholar]
  15. Elhag KM, Bahar AM, Mahajan KK. Difficulties in the serodiagnosis of infection with the fragilis group of Bacteroides. J Med Microbiol 1987; 24:125–131
    [Google Scholar]
  16. Danielsson D, Lambe DW, Persson S. Immune response to anaerobic infections. Ballows A, De Haan RM, Dowell VR, Gurse LB. ed Anaerobic bacteria: Role in disease Springfield IL: Charles C. Thomas; 1974173–191
    [Google Scholar]
  17. Hofstad T. Antibodies reacting with lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis and Fusobacterium nucleatum in serum from normal human subjects. J Infect Dis 1974; 129:349–352
    [Google Scholar]
  18. Holdeman LV, Cato EP, Moore WEC. eds Anaerobe Laboratory manual, 4th. Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg, VA: 1977
    [Google Scholar]
  19. Alkarmi T, Ali-Khan Z, Zarkados C. Characterization of amyloid protein from chronic hydatid mice: isolation, purification and amino acid analysis. Exp Mol Pathol 1985; 45:142–159
    [Google Scholar]
  20. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
  21. Norusis MJ. Cluster analysis; proximities. SPSSX: Advanced statistics guide New York: McGraw-Hill Company; 1985165–191462–467
    [Google Scholar]
  22. Cherniak R, Lombard GL, Dowell VR. Immunochemical evidence of multiple serotypes of Bacteroides fragilis. J Clin Microbiol 1979; 9:699–704
    [Google Scholar]
  23. Romond Ch, Beerens H, Wattre P. Serological identification of bacteroides connected with their pathogenicity. Arch Roum Pathol Exp Microbiol 1972; 31:351–355
    [Google Scholar]
  24. Kasper DL. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis 1976; 133:79–87
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-35-2-118
Loading
/content/journal/jmm/10.1099/00222615-35-2-118
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error