1887

Abstract

Summary

Methicillin-resistant strains of isolated during 1985 and 1987 at a Nigerian hospital were compared by resistance profiles, plasmid analysis, and pulsed-field gel electrophoresis of chromosomal DNA. The results indicated that the isolates from the two periods were unrelated with regard to all three aspects. None of the isolates was similar to the classical MRSA nor to the epidemic MRSA of Australia or the UK. The MRSA isolated in 1985 had a similar plasmid to MRSA isolates from Singapore, but differed from them when compared by pulsed-field gel electrophoresis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-3-203
1993-03-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/3/medmicro-38-3-203.html?itemId=/content/journal/jmm/10.1099/00222615-38-3-203&mimeType=html&fmt=ahah

References

  1. Shanson DC. Antibiotic-resistant Staphylococcus aureus. J Hosp Infect 1981; 2:11–36
    [Google Scholar]
  2. Jevons PM. Celbenin-resistant staphylococci. Br Med Bull 1961; 1:124–125
    [Google Scholar]
  3. Barber M. Methicillin-resistant staphylococci. J Clin Pathol 1961; 14:385–393
    [Google Scholar]
  4. Knox R. Celbenin-resistant staphylococci. Br Med Bull 1961; 1:126
    [Google Scholar]
  5. Selkon JB, Stokes ER, Ingham HR. The role of an isolation unit in the control of hospital infection with methicillin-resistant staphylococci. J Hasp Infect 1980; 1:41–46
    [Google Scholar]
  6. El Solh N, Fouace JM, Pillet J, Chabbert YA. Plasmid DNA content of multi-resistant Staphylococcus aureus strains. Ann Microbiol (Paris) 1981; 132B:131–156
    [Google Scholar]
  7. Haley RW, Hightower AW, Khabbaz RF. et al. The emergence of methicillin-resistant Staphylococcus aureus in United States hospitals. Ann Intern Med 1982; 97:297–308
    [Google Scholar]
  8. Cafferkey MT, Hone R, Falkiner FR, Keane CT, Pomeroy H. Gentamicin and methicillin-resistant Staphylococcus aureus in Dublin hospitals: clinical and laboratory studies. J Med Microbiol 1983; 16:117–127
    [Google Scholar]
  9. Coleman DC, Pomeroy H, Estridge JK. et al. Susceptibility to antimicrobial agents and analysis of plasmids in gentamicin- and methicillin-resistant Staphylococcus aureus from Dublin hospitals. J Med Microbiol 1985; 20:157–167
    [Google Scholar]
  10. Pavillard R, Harvey K, Douglas D. et al. Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Med J Aust 1982; 1:451–454
    [Google Scholar]
  11. Wenzel RP. The emergence of methicillin-resistant Staphylococcus aureus. Ann Intern Med 1982; 97:440–442
    [Google Scholar]
  12. Lacey RW, Grinsted J. Genetic analysis of methicillin-resistant strains of Staphylococcus aureus, evidence for their evolution from a single clone. J Med Microbiol 1973; 6:511–526
    [Google Scholar]
  13. Lyon BR, Iuorio JL, May JW, Skurray RA. Molecular epidemiology of multirésistant Staphylococcus aureus in Australian hospitals. J Med Microbiol 1984; 17:79–89
    [Google Scholar]
  14. Townsend DE, Grubb WB, Ashdown N. Genetics of drug resistance in methicillin-resistant Staphylococcus aureus from Australian hospitals. J Hosp Infect 1983; 4:331–337
    [Google Scholar]
  15. Grubb WB. Molecular epidemiology of methicillin-resistant Staphylococcus aureus.. In: Novick RN. (ed) Molecular biology of staphylococci New York: VCH Publishers; 1990595–606
    [Google Scholar]
  16. Townsend DE, Ashdown N, Bolton S. et al. The international spread of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1987; 9:60–71
    [Google Scholar]
  17. Wei M-Q, Groth DM, Mendis AHW, Sampsom J, Wetherall JD, Grubb WB. Typing of methicillin-resistant Staphylococcus aureus with a Ml3 repeat probe. J Hosp Infect 1992; 20:233–245
    [Google Scholar]
  18. Wei M-Q, Wang F, Grubb WB. Use of contour-clamped homogeneous electric field electrophoresis to type methicillin-resistant Staphylococcus aureus. J Med Microbiol 1992; 36:172–176
    [Google Scholar]
  19. Rotimi VO, Orebamjo OA, Banjo TO, Onyenefa PI, Nwobu RN. Occurrence and antibiotic susceptibility profiles of methicillin-resistant Staphylococcus aureus in Lagos University Teaching Hospital. Cent AfrJ Med 1987; 33:95–99
    [Google Scholar]
  20. Ako-Nai AK, Oguniyi AD, Lamikanra A, Torimiro SEA. The characterisation of clinical isolates of Staphylococcus aureus in Ile-Ife Nigeria. J Med Microbiol 1991; 34:109–112
    [Google Scholar]
  21. Townsend DE, Grubb WB, Ashdown N. Gentamicin resistance in methicillin resistant Staphylococcus aureus. Pathology 1983; 15:169–174
    [Google Scholar]
  22. Annear DI, Grubb WB. Linked and unstable resistance to kanamycin and penicillin and diffusible pigment production, in an isolate of Staphylococcus aureus. J Med Microbiol 1972; 5:109–111
    [Google Scholar]
  23. Udo EE, Grubb WB. Excision of a conjugative plasmid from the staphylococcal chromosome. J Med Microbiol 1990; 33:227–234
    [Google Scholar]
  24. O’Callaghan CH, Morris A, Kirby SM, Shingler AH. Novel method for detection of beta-lactamase by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemo-ther 1972; 1:283–288
    [Google Scholar]
  25. Townsend DE, Ashdown N, Bolton S, Grubb WB. The use of cetyltrimethylammonium bromide for the isolation from Staphylococcus aureus of relaxable and non relaxable plasmid DNA for in vitro manipulation. Lett Appl Microbiol 1985; 1:87–94
    [Google Scholar]
  26. Truchsis M, Wolfson JS, Hooper DC. A novel locus conferring fluoroquinolone resistance in Staphylococcus aureus. J Bacteriol 1991; 173:5854–5860
    [Google Scholar]
  27. Grubb WB, Townsend DE, Ashdown N, Tjia T, McGlashan C, Leng T. Genetic analysis of methicillin-resistant Staphylococcus aureus from Singapore hospitals. Eur J Clin Microbiol 1986; 5:728–730
    [Google Scholar]
  28. Utsalo SJ. Characterisation of hospital and community strains of Staphylococcus aureus for resistance to antimicrobial drugs, metallic ions, disinfectants, thermal injury and solar radiation. Acta Microbiol Hung 1986; 33:183–191
    [Google Scholar]
  29. Wemambu SNC. Wound infections and nasal colonisation of staff with Staphylococcus aureus in Benin, Nigeria. J Hosp Infect 1981; 2:259–260
    [Google Scholar]
  30. Rotimi VO, Odugbemi TO, Fadahunsi O, Ogunbi O. Penicillin resistance in Staphylococcus aureus: prevalence of penicillinase producing strains in Lagos University Teaching Hospital. Nig Med J 1979; 9:307–310
    [Google Scholar]
  31. Nwobu RA, Ogunbi OO, Rotimi VO. Phage-types and re sistance pattern of Staphylococcus aureus isolated from clinical specimens to penicillin and cloxacillin in a Lagos hospital. Cent Afr J Med 1986; 32:155–158
    [Google Scholar]
  32. Grinsted J, Lacey RW. Genetic variation of streptomycin resistance in clinical strains of Staphylococcus aureus. J Med Microbiol 1973; 6:351–361
    [Google Scholar]
  33. Kayser FH, Wust J, Corrodi P. Transduction and elimination of resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1972; 2:217–223
    [Google Scholar]
  34. Kayser FH, Wust J, Santanam P. Genetic and molecular characterisation of resistance determinants in methicillin-resistant Staphylococcus aureus. J Med Microbiol 1976; 9:137–147
    [Google Scholar]
  35. Matthews PR, Reed KC, Stewart PR. The cloning of chromo somal DNA associated with methicillin and other resistances in Staphylococcus aureus. J Gen Microbiol 1987; 133:1919–1929
    [Google Scholar]
  36. Beck WD, Berger-Bachi B, Kayser FH. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. J Bacteriol 1986; 165:373–378
    [Google Scholar]
  37. Udo EE, Grubb WB. Transposition of genes encoding kana mycin, neomycin and streptomycin resistance in Staphylococcus aureus. J Antimicrob Chemother 1991; 27:713–720
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-3-203
Loading
/content/journal/jmm/10.1099/00222615-38-3-203
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error