1887

Abstract

Summary

The opsonic requirements of were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of with pooled normal human serum (NHS) in concentrations of 5–20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the bacteria with NHS 30% caused the release of radiolabel and this effect was abolished by heating serum to 56°C, suggesting that is serum-sensitive and that complement is involved. Opsonisation of with NHS concentrations of 10–30% significantly increased PMNL chemi-luminescence. Removal of specific antibody had no effect. Removal of either the classical or alternative complement pathways produced no significant change in PMNL chemilum-inescence, indicating that either pathway is sufficient for opsonisation on its own. The results confirm that complement is the most efficient opsonin for

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1993-03-01
2024-04-27
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References

  1. (a) Warren JR, (b) Marshall BJ. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983; 1:1273–1275 (two letters)
    [Google Scholar]
  2. Goodwin CS, Armstrong JA, Chilvers T. et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Campylobacter mustelae comb, nov., respectively. Int J Syst Bacteriol 1989; 39:397–405
    [Google Scholar]
  3. Wyatt JI, Dixon MF. Chronic gastritis–a pathogenetic ap proach. J Pathol 1988; 154:113–124
    [Google Scholar]
  4. Shousha S, Bull TB, Parkins RA. Gastric spiral bacteria. Lancet 1984; 2:101
    [Google Scholar]
  5. Newell DG. Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C.pyloridis and C. jejuni. J Gen Microbiol 1987; 133:163–170
    [Google Scholar]
  6. Wyatt JI, Rathbone BJ, Heatley RV. Local immune response to gastric Campylobacter in non-ulcer dyspepsia. J Clin Pathol 1986; 39:863–870
    [Google Scholar]
  7. Mai UEH, Perez-Perez GI, Wahl LM, Wahl SM, Blaser MJ, Smith PD. Soluble surface proteins from Helicobacter pylori activate monocytes/macrophages by lipopoly-saccharide-independent mechanism. J Clin Invest 1991; 87:894–900
    [Google Scholar]
  8. Goodwin CS, Blincow ED, Warren JR, Waters TE, Sanderson CR, Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol 1985; 38:1127–1131
    [Google Scholar]
  9. Bolton FJ, Hutchinson DN, Hinchliffe PM, Holt AV. Dis tribution in various clinical groups of antibody to C. pylori detected by enzyme-linked immunosorbent assay, complement fixation and microagglutination tests. Serodiagn Immunotherap Infect Dis 1989; 3:41–50
    [Google Scholar]
  10. Bolton FJ, Hutchinson DN. Evaluation of three Campylobacter pylori antigen preparations for screening sera from patients undergoing endoscopy. J Clin Pathol 1989; 42:723–726
    [Google Scholar]
  11. Götze O, Müller-Eberhard HJ. The C3-activator system: an alternate pathway of complement activation. J Exp Med 1971; 134:90s–108s
    [Google Scholar]
  12. Fine DP, Mamey SR, Colley DG, Sergent JS, Des Prez RM. C3 Shunt activation in human serum chelated with EGTA. J Immunol 1972; 109:807–809
    [Google Scholar]
  13. Jonsson S, Kronvall G. The use of protein A containing Staphylococcus aureus as a solid phase anti-IgG reagent in radioimmuno assays as exemplified in the quantitation of a-fetoprotein in normal human adult serum. Eur J Immunol 1974; 4:29–33
    [Google Scholar]
  14. Boyurn A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 1968; 21:Suppl 9777–89
    [Google Scholar]
  15. Verhoef J, Peterson PK, Quie PG. Kinetics of Staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using (3H) thymidine labeled bacteria. J Immunol Methods 1977; 14:303–311
    [Google Scholar]
  16. Armitage P, Berry G. Statistical methods in medical research. Oxford: Blackwell Scientific Publications;, 2nd edn.. 1987
    [Google Scholar]
  17. Das SS, Karim QN, Easmon CSF. Opsonophagocytosis of Campylobacter pylori. J Med Microbiol 1988; 27:125–130
    [Google Scholar]
  18. Pruul H, Lee PC, Goodwin CS, McDonald PJ. Interaction of Campylobacter pyloridis with human immune defence mechanisms. J Med Microbiol 1987; 23:233–238
    [Google Scholar]
  19. Henricks PAJ, van der Toi ME, Thyssen RMWM, van Asbeck BS, Verhoef J. Escherichia coli lipopolysaccharides diminish and enhance cell function of human polymorphonuclear leukocytes. Infect Immun 1983; 41:294–301
    [Google Scholar]
  20. Gemmell CG, Peterson PK, Townsend K, Quie PG, Youngki K. Biological effects of the interaction of Staphylococcal a-toxin with human serum. Infect Immun 1982; 38:981–985
    [Google Scholar]
  21. Hatch GE, Gardner DE, Menzel DB. Chemiluminescence of phagocytic cells caused by N-formylmethionyl peptides. J Exp Med 1978; 147:182–195
    [Google Scholar]
  22. Newell DG, Stacey AR. The serology of Campylobacter pylori infections. In: Rathbone BJ, Heatley RV. (eds) Campylobacter pylori and gastroduodenal disease Oxford: Blackwell Scientific Publications; 198974–82
    [Google Scholar]
  23. Kazi JI, Sinniah R, Jaffrey NA. et al. Cellular and humoral immune responses in Campylobacter pylori-associated chronic gastritis. J Pathol 1989; 159:231–237
    [Google Scholar]
  24. Rathbone BJ, Wyatt JI, Worsley BW. et al. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut 1986; 27:642–647
    [Google Scholar]
  25. Topley N, Alobaidi HM, Davies M, Coles GA, Williams JD, Lloyd D. The effect of dialysate on peritoneal phagocyte oxidative metabolism. Kidney Int 1988; 34:404–411
    [Google Scholar]
  26. van Bronswijk H, Verbrugh HA, Heezius HC, van der Meulen J, Oe PL, Verhoef J. Dialysis fluids and local host resistance in patients on continuous ambulatory peritoneal dialysis. Eur J Clin Microbiol Infect Dis 1988; 7:368–373
    [Google Scholar]
  27. Hazell SL, Lee A. The adaption of motile strains of Campylo bacter pyloridis to gastric mucus and their association with gastric epithelial intracellular spaces. In: Pearson AD, Skirrow MB, Lior H, Rowe B. (eds) Campylobacter III: Proceedings of the third international workshop on Campylobacter infections, Ottawa, 7–15th July 1985 London: Public Health Laboratory Service;
    [Google Scholar]
  28. Megraud F. Campylobacter pylori: Enzymes. In: Rathbone BJ, Heatley RV. (eds) Campylobacter pylori and gastroduodenal disease Oxford: Blackwell Scientific; 198939–47
    [Google Scholar]
  29. Leunk RD, Johnson PT, David BC, Kraft WG, Morgan DR. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol 1988; 26:93–99
    [Google Scholar]
  30. Babiour BM. Oxygen dependent killing by phagocytes. N Engl J Med 1978; 298:659–668
    [Google Scholar]
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