1887

Journal of Medical Microbiology logo

Volume 38, Issue 3

Naturally-occurring, osmo-remedial variants of Free

Abstract

Summary

Two clones of O27:K1:H31 and O2:H7, isolated from patients with urinary tract infection or bacteraemia, failed to grow in a synthetic minimal medium (MM) of low osmolality. They were considered to be osmo-remedial because they grew well when sufficient amounts of NaCl, mannitol or sucrose were added to raise the osmolality of the medium to > 300 mOsm/kg. The defect could also be corrected by nicotinamide or its precursors quinolinic and aspartic acids. Each clone had a unique DNA restriction enzyme profile, fimbriae and antibiotic susceptibility patterns. The osmo-remedial variants were unstable and underwent phenotypic modulation to form mixtures with osmo-tolerant forms when grown in MM. They tended to form satellites of small colonies around large colonies of osmo-tolerant cells on MM agar plates. The penicillin method of Davis was used to separate the two forms. Nicotinamide induced the expression of when the osmo-remedial strains were grown under conditions of low osmolality. It is possible that the variants are defective in the synthesis of membrane-derived oligosaccharides or outer-membrane proteins, but this has yet to be determined.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology, 1993
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-3-216
1993-03-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/3/medmicro-38-3-216.html?itemId=/content/journal/jmm/10.1099/00222615-38-3-216&mimeType=html&fmt=ahah

References

  1. Ingraham J. Effect of temperature, pH, water activity, and pressure on growth. In: Neidhardt FC. (ed) Escherichia coli and Salmonella typhimurium cellular and molecular biology vol 2 Washington: American Society for Microbiology; 19871543–1554
     [Google Scholar]
  2. Achtman M, Mercer A, Kusecek B. et al. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun 1983; 39:315–335
     [Google Scholar]
  3. Nimmich W, Zingler G. Biochemical characteristics, phage patterns, and O1 factor analysis of Escherichia coli O1:K1:H7:F11 and O1:K1:H−:F9 strains isolated from patients with urinary tract infections. Med Microbiol Immunol 1984; 173:75–85
     [Google Scholar]
  4. Robeson JP, Goldschmidt RM, Curtiss R. Potential of Escheri chia coli isolated from nature to propagate cloning vectors. Nature 1980; 283:104–106
     [Google Scholar]
  5. Gillespie WA. Biochemical mutants of coliform bacilli in infections of the urinary tract. J Pathol Bacterio1 1952; 64:551–557
     [Google Scholar]
  6. Mclver CJ, Tapsall JW. Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli. Pathology 1987; 19:361–363
     [Google Scholar]
  7. Davis BD. The isolation of biochemically deficient mutants of bacteria by means of penicillin. Proc Natl Acad Sci USA 1949; 35:1–10
     [Google Scholar]
  8. Le Rudulier D, Strom AR, Dandekar AM, Smith LT, Valentine RC. Molecular biology of osmoregulation. Science 1984; 224:1064–1068
     [Google Scholar]
  9. Davis BD, Mingioli ES. Mutants of Escherichia coli requiring methionine or vitamin B12 . J Bacteriol 1950; 60:17–28
     [Google Scholar]
  10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
     [Google Scholar]
  11. Van Ketel RJ, ter Schegget J, Zanen HC. Molecular epi demiology of Legionella pneumophila serogroup 1. J Clin Microbiol 1984; 20:362–364
     [Google Scholar]
  12. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor NY: Cold Spring Harbor Laboratory; 1982; 451
     [Google Scholar]
  13. Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145:1365–1373
     [Google Scholar]
  14. Kennedy EP. Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli. Proc Natl Acad Sci USA 1982; 79:1092–1095
     [Google Scholar]
  15. Barron A, May G, Bremer E, Villarejo M. Regulation of envelope protein composition during adaptation to osmotic stress in Escherichia coli. J Bacteriol 1986; 167:433–438
     [Google Scholar]
  16. Lugtenberg B, Peters R, Bernheimer A, Benendson W. Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Mol Gen Genet 1976; 147:251–262
     [Google Scholar]
  17. Van Alphen WV, Lugtenberg B. Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli. J Bacteriol 1977; 131:623–630
     [Google Scholar]
  18. Jaffe A, Chabbert YA, Semonin O. Role of porin proteins OmpF and OmpC in the permeation of beta-lactams. Antimicrob Agents Chemother 1982; 22:942–948
     [Google Scholar]
  19. Nikaido H, Rosenberg EY, Foulds J. Porin channels in Escherichia coli: studies with β-lactams in intact cells. J Bacteriol 1983; 153:232–240
     [Google Scholar]
  20. Fielder W, Rotering H. Properties of Escherichia coli mutants lacking membrane-derived oligosaccharides. J Biol Chem 1988; 263:14684–14689
     [Google Scholar]
  21. Geiger O, Russo FD, Silhavy TJ, Kennedy EP. Membranederived oligosaccharides affect porin osmoregulation only in media of low ionic strength. J Bacteriol 1992; 174:1410–1413
     [Google Scholar]
  22. Young K, Silver LL. Leakage of periplasmic enzymes from envAl strains of Escherichia coli. J Bacteriol 1991; 173:3609–3614
     [Google Scholar]
  23. Kohno T, Roth J. Electrolyte effects on the activity of mutant enzymes in vivo and in vitro. Biochemistry 1979; 18:1386–1392
     [Google Scholar]
  24. Russell RRB. Temperature-sensitive osmotic remedial mutants of Escherichia coli. J Bacteriol 1972; 112:661–665
     [Google Scholar]
  25. Fincham JRS, Baron AJ. The molecular basis of an osmotically reparable mutant of Neurospora crassa producing unstable glutamate dehydrogenase. J Mol Biol 1977; 110:627–642
     [Google Scholar]
  26. Martin CE, DeBusk AG. Temperature-sensitive, osmoticremedial mutants of Neurospora crassa: osmotic pressure induced alterations of enzyme stability. Mol Gen Genet 1975; 136:31–40
     [Google Scholar]
  27. Ryan FJ, Kunin CM, Ballentine R, Maas W. Unique sensitivity of a panthothenicless mutant of Neurospora to constituents of the growth medium. J Bacteriol 1953; 65:434–439
     [Google Scholar]
  28. Hawthorne DC, Friis J. Osmotic-remedial mutants. A new classification for nutritional mutants in yeast. Genetics 1964; 50:829–839
     [Google Scholar]
  29. Eisenstein BI. Type 1 fimbriae of Escherichia coli: genetic regulation, morphogenesis, and role in pathogenesis. Rev Infect Dis 1988; 10:Suppl 2S341–S344
     [Google Scholar]
  30. Weiss AA, Hewlett EL. Virulence factors for Bordetella pertussis. Annu Rev Microbiol 1986; 40:661–686
     [Google Scholar]
  31. Chambers HF, Hackbarth CJ. Effect of NaCl and nafcillin on penicillin-binding protein 2a and heterogeneous expression of methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 1987; 31:1982–1988
     [Google Scholar]
  32. Weiner M, Van Eys J. Nicotinic acid. Nutrient-cofactor-drug New York: Marcel Dekker, Inc; 1983
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-3-216
Loading
Naturally-occurring, osmo-remedial variants of Escherichia coli
J. Med. Microbiol. 38, 216 (1993); https://doi.org/10.1099/00222615-38-3-216
/content/journal/jmm/10.1099/00222615-38-3-216
/content/journal/jmm/10.1099/00222615-38-3-216
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error