1887

Abstract

Summary

The classical techniques of M protein and opacity factor (OF) typing and T agglutination typing remain the “gold standard” in identifying group A streptococci, although newer techniques have been proposed to assist laboratory scientists, microbiologists, epidemiologists and clinicians in the precise identification and characterisation of these organisms. Because of the current scarcity of M-typing sera and the increased use by many laboratories of T typing as the sole method of group A identification, a table is presented to indicate specific correlation between the T-agglutination pattern and the M serotype. The use of this table will enable not only more selective use of typing sera but also, perhaps, result in improved understanding and ultimately in correlating these defined patterns with newer and more sensitive techniques.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-5-311
1993-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/5/medmicro-38-5-311.html?itemId=/content/journal/jmm/10.1099/00222615-38-5-311&mimeType=html&fmt=ahah

References

  1. Tagg JR, Bannister LV. “ Fingerprinting ” β-haemolytic strepto cocci by their production of and sensitivity to bacteriocine- like inhibitors. J Med Microbiol 1979; 12:397–411
    [Google Scholar]
  2. Cleary PP, Kaplan EL, Livdahl C, Skjold S. DNA fingerprints of Streptococcus pyogenes are M type specific. J Infect Dis 1988; 158:1317–1323
    [Google Scholar]
  3. Musser JM, Gray BM, Schlievert PM, Pichichero ME. Strep tococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing. J Clin Microbiol 1992; 30:600–603
    [Google Scholar]
  4. Single LA, Martin DR. Clonal differences within M-types of the group A streptococcus revealed by pulsed field gel electrophoresis. FEMS Microbiol Lett 1992; 70:85–90
    [Google Scholar]
  5. Kafhold A, Podbielski A, Johnson DR, Kaplan EL, Lutticken R. M-protein gene typing of Streptococcus pyogenes by nonradioactively labeled oligonucleotide probes. J Clin Microbiol 1992; 30:2391–2397
    [Google Scholar]
  6. Rotta J. Group and type (groups A and B) identification of haemolytic streptococci.. In Bergan T, Norris JR. eds Methods in microbiology, vol 12. London: >Academic Press; 1978177–198
    [Google Scholar]
  7. Lancefield RC. Current knowledge of type-specific M antigens of group A streptococci. J Immunol 1962; 89307–313
    [Google Scholar]
  8. Facklam RR, Edwards LR. A reference laboratory’s investi gations of proposed M-type strains of Streptococcus pyogenes, capsular types of S. agalactiae, and new group antigens of streptococci.. In Parker MT. ed Pathogenic streptococci. Chertsey, UK: Reedbooks; 1979251–253
    [Google Scholar]
  9. Moody MD, Padula J, Lizana D, Hall CT. Epidemiologic characterization of group A streptococci by T-aggluti- nation and M-precipitation tests in the public health laboratory. Hlth Lab Sci 1965; 2:149–162
    [Google Scholar]
  10. Maxted WR, Widdowson JP, Fraser CAM, Ball LC, Bassett DCJ. The use of the serum opacity reaction in the typing of group-A streptococci. J Med Microbiol 1973; 6:83–90
    [Google Scholar]
  11. Johnson DR, Kaplan EL. Microtechnique for serum opacity factor characterization of group A streptococci adaptable to the use of human sera. J Clin Microbiol 1988; 26:2025–2030
    [Google Scholar]
  12. Lancefield R. Type specific antigens, M and T, of matt and glossy variants of group A hemolytic streptococci. J Exp Med 1940; 71:521–537
    [Google Scholar]
  13. Williams REO. Laboratory diagnosis of streptococcal in fections. Bull WldHlth Org 1958; 19:153–176
    [Google Scholar]
  14. Efstratiou A. Preparation of Streptococcus pyogenes suspen sions for typing by the agglutination method. Med Lab Sci 1980; 37:361–363
    [Google Scholar]
  15. Lancefield RC. The antigenic complex of streptococcus haemo-lyticus. I. Demonstration of a type-specific substance in extracts of Streptococcus haemolyticus . J Exp Med 1928; 47:91–103
    [Google Scholar]
  16. Swift HF, Wilson AT, Lancefield RC. Typing group A hemolytic streptococci by M precipitin reactions in capillary pipettes. J Exp Med 1943; 78:127–133
    [Google Scholar]
  17. Rotta J, Krause RM, Lancefield RC, Everly W, Lackland H. New approaches for the laboratory recognition of M-types of group A streptococci. J Exp Med 1971; 134:1298–1315
    [Google Scholar]
  18. Top FH, Wannamaker LW. The serum opacity reaction of Streptococcus pyogenes: the demonstration of multiple, strain-specific lipoproteinase antigens. J Exp Med 1968; 127:1013–1034
    [Google Scholar]
  19. Fraser CAM. Preparation of specific antisera to the opacity factors of group A streptococci. J Med Microbiol 1982; 15:153–162
    [Google Scholar]
  20. Maxted WR, Valkenburg HA. Variation in the M-antigen of group-A streptococci. J Med Microbiol 1969; 2:199–210
    [Google Scholar]
  21. Rotta J, Facklam RR. Manual of microbiological diagnostic methods for streptococcal infections and their sequelae (with basic information on the streptococcus problem). Geneva: World Health Organization; 1980
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-5-311
Loading
/content/journal/jmm/10.1099/00222615-38-5-311
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error