1887

Abstract

Summary

Strains of isolated from children in South Australia and the Northern Territory with systemic infections (mostly meningitis or epiglottitis) were subjected to serotyping, biotyping, outer-membrane protein (OMP) analysis and immunoblot subtyping. All 65 isolates examined were from blood or cerebrospinal fluid; 59 (91%) of the strains were identified as type b and the remainder as either type a (two strains) or non-typable (four strains). Of the 59 type b strains, 45 (76%) belonged to a single OMP subtype (equivalent to subtype 3L in the Barenkamp scheme); the remaining type b strains belonged to five other OMP subtypes. No correlation was apparent between OMP subtype and geographical region, clinical diagnosis or antimicrobial drug susceptibility pattern. Immunoblot subtyping enabled nine (18%) of 41 strains belonging to the principal OMP subtype to be distinguished from the remainder.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-5-378
1993-05-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/5/medmicro-38-5-378.html?itemId=/content/journal/jmm/10.1099/00222615-38-5-378&mimeType=html&fmt=ahah

References

  1. Albritton WL, Penner S, Slaney L, Brunton J. Biochemical characteristics of Haemophilus influenzae in relationship to source of isolation and antibiotic resistance. J Clin Microbiol 1978; 7:519–523
    [Google Scholar]
  2. Loeb MR, Smith DH. Outer membrane protein composition in disease isolates of Haemophilus influenzae: Pathogenic and epidemiological implications. Infect Immun 1980; 30:709–717
    [Google Scholar]
  3. Barenkamp SJ, Munson RS, Granoff DM. Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles. J Infect Dis 1981; 143:668–676
    [Google Scholar]
  4. van Alphen L, Riemens T, Poolman J, Zanen HC. Character istics of major outer membrane proteins of Haemophilus influenzae . J Bacteriol 1983; 155:878–885
    [Google Scholar]
  5. van Alphen L, Riemens T, Poolman J, Hopman C, Zanen HC. Homogeneity of cell envelope protein subtypes, lipo- polysaccharide serotypes, and biotypes amongst Haemophilus influenzae type b from patients with meningitis in the Netherlands. J Infect Dis 1983; 148:75–81
    [Google Scholar]
  6. van Alphen L, Geelen L, Jonsdottir K, Takala AK, Kayhty H, Zanen HC. Distinct geographic distribution of subtypes of Haemophilus influenzae type b in Western Europe. J Infect Dis 1987; 156:216–218
    [Google Scholar]
  7. Mulligan ME, Peterson LR, Kwok RYY, Clabots CR, Gerding DN. Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile . J Clin Microbiol 1988; 26:41–46
    [Google Scholar]
  8. Hansman D, Hanna J, Morey F. High prevalence of invasive Haemophilus influenzae disease in Central Australia. Lancet 1986; 2:927
    [Google Scholar]
  9. McIntyre P, Wheaton G, Erlich J, Hansman D. Brasilian purpuric fever in Central Australia. Lancet 1987; 2:112
    [Google Scholar]
  10. Wild BE, Pearman JW, Campbell PB, Swan PK, Gurry DL. Brazilian purpuric fever in Western Australia. Med J Aust 1989; 150:344–346
    [Google Scholar]
  11. Wilson GS, Miles A. Topley and Wilson’s Principles of bacteriology, virology and immunity, 6th edn. London: Edward Arnold; 1975; 321022
    [Google Scholar]
  12. Kilian M. Haemophilus. In Lennette H. ed Manual of clinical microbiology, 4th edn. Washington DC: American Society for Microbiology; 1985387–393
    [Google Scholar]
  13. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  14. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-5-378
Loading
/content/journal/jmm/10.1099/00222615-38-5-378
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error