1887

Journal of Medical Microbiology logo

Volume 42, Issue 1

Temperature-dependent protein and lipopolysaccharide expression in clinical isolates Free

Abstract

Surmmary

Clinical isolates of were grown at eight different temperatures from 10°C to 40°C. Whole cell lysates were examined by SDS-PAGE and major temperature-dependent changes to both protein and lipopolysaccharide (LPS) profiles were identified. Cells grown at the higher temperatures (37°C and 40°C) produced abundantly a protein of . 60 kDa which was not detected at the lower temperatures. Temperature-dependent expressions of other proteins were also noted but these were more variable among the isolates. An effect of temperature on expression of lipopolysaccharides was also noted in that some strains produced significantly less O-polysaccharides at the higher temperatures. After fractionation of cells, major differences in the expression of cell envelope and outer-membrane proteins between cells grown at low and high temperatures were noted although no unifying patterns could be discerned. Such growth temperature-induced changes in the cell envelope constituents have not been described previously for isolates from man.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1995
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-1-32
1995-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/1/medmicro-42-1-32.html?itemId=/content/journal/jmm/10.1099/00222615-42-1-32&mimeType=html&fmt=ahah

References

  1. Janda J. M., Duffey P. S. Mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum. Rev Infect Dis 1988; 10:980–997
     [Google Scholar]
  2. Palumbo S. A. The occurrence and significance of organisms of the Aeromonas hydrophila group in food and water. Med Microbiol Lett 1993; 2:339–346
     [Google Scholar]
  3. Asao T., Kinoshita Y., Kozaki S., Uemura T., Sakaguchi G. Purification and some properties of Aeromonas hydrophila hemolysin. Infect Immun 1984; 46:122–127
     [Google Scholar]
  4. Chopra A. K., Ferguson M. R., Houston C. W. Molecular characterization of enterotoxins from Aeromonas hydrophila. Med Microbiol Lett 1993; 2:261–268
     [Google Scholar]
  5. Ljungh A. H. Toxins of Aeromonas hydrophila. In Alouf J. E., Fehrenbach F. J., Freer J. H., Jeljaszewicz J. (eds) Bacterial protein toxins London: Academic Press; 1984309–315
     [Google Scholar]
  6. Nieto T. P., Santos Y., Rodriguez L. A., Ellis A. E. An extracellular acetylcholinesterase produced by Aeromonas hydrophila is a major lethal toxin for fish. Microb Pat hog 1991; 11:101–110
     [Google Scholar]
  7. Janda J. M. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev 1991; 4:397–410
     [Google Scholar]
  8. Maurelli A. T., Blackmon B., Curtiss R. Temperature-dependent expression of virulence genes in Shigella species. Infect Immun 1984; 43:195–201
     [Google Scholar]
  9. Cornelis G. R. Yersinae, finely tuned pathogens. In Hormaeche C. E., Penn C. W., Smyth C. J. (eds) Molecular biology of bacterial infection, current status and future perspectives, Symposium 49, Society for General Microbiology Cambridge: Cambridge University Press; 1992231–265
     [Google Scholar]
  10. Mateos D., Anguita J., Naharro G., Paniaguer C. Influence of growth temperature on the production of extracellular virulence factors and pathogenicity of environmental and human strains of Aeromonas hydrophila. J Appl Bacteriol 1993; 74:111–118
     [Google Scholar]
  11. George W. L., Jones M. J., Nakata M. M. Phenotypic characteristics of Aeromonas species isolated from adult humans. J Clin Microbiol 1986; 23:1026–1029
     [Google Scholar]
  12. Kuijper E. J., Steigerwalt A. G., Schoenmakers B. S., Peeters M. F., Zanen H. C., Brenner D. J. Phenotypic characterization and DNA relatedness in human faecal isolates of Aeromonas spp. J Clin Microbiol 1989; 27:132–138
     [Google Scholar]
  13. Merino S., Camprubi S., Tomas J. M. Effect of growth temperature on outer membrane components and virulence of Aeromonas hydrophila strains of serotype O:34. Infect Immun 1992; 60:4343–4349
     [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
     [Google Scholar]
  15. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 1983; 154:269–277
     [Google Scholar]
  16. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115–119
     [Google Scholar]
  17. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol 1973; 115:717–722
     [Google Scholar]
  18. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248–254
     [Google Scholar]
  19. Statner B., Jones M. J., George W. L. Effect of incubation temperature on growth and soluble protein profiles of motile Aeromonas strains. J Clin Microbiol 1988; 26:392–393
     [Google Scholar]
  20. Kokka R. P., Vedros N. A., Janda J. M. Electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative Aeromonas hydrophila and Aeromonas sobria. J Clin Microbiol 1990; 28:2240–2247
     [Google Scholar]
  21. Dooley J. S. G., Lallier R., Shaw D. H., Trust T. J. Electrophoretic and immunochemical analysis of the lipopolysaccharides from various strains of Aeromonas hydrophila. J Bacteriol 1985; 164:263–269
     [Google Scholar]
  22. Dooley J. S. G., Trust T. J. Surface protein composition of Aeromonas hydrophila strains virulent for fish: identification of a surface array protein. J Bacteriol 1988; 170:499–506
     [Google Scholar]
  23. Schubert R. H. W., Matzinou D. Temperature as an environmental factor influencing the pathogenicity of Aeromonas hydrophila. Int J Med Microbiol 1990; 273:327–331
     [Google Scholar]
  24. Millership S. E., Want S. V. Characterisation of strains of Aeromonas spp. by phenotype and whole-cell protein fingerprint. J Med Microbiol 1993; 39:107–113
     [Google Scholar]
  25. Kokka R. P., Vedros N. A., Janda J. M. Characterization of classic and atypical serogroup O:11 Aeromonas: evidence that the surface array protein is not directly involved in mouse pathogenicity. Microb Pathogen 1991; 10:71–79
     [Google Scholar]
  26. Kokka R. P., Vedros N. A., Janda J. M. Immunochemical analysis and possible biological role of an Aeromonas hydrophila surface array protein in septicaemia. J Gen Microbiol 1992; 138:1229–1236
     [Google Scholar]
  27. Popoff M. Y. Genus III: Aeromonas Kluyver and Van Niel 1936. In Kreig N. R., Holt S. G. (eds) Bergey’s Manual of systematic bacteriology 9th edn Baltimore: Williams and Wilkins Co; 1984545–548
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-1-32
Loading
Temperature-dependent protein and lipopolysaccharide expression in clinical Aeromonas isolates
J. Med. Microbiol. 42, 32 (1995); https://doi.org/10.1099/00222615-42-1-32
/content/journal/jmm/10.1099/00222615-42-1-32
/content/journal/jmm/10.1099/00222615-42-1-32
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error