f Characteristics of invasion of HEp-2 cells by Providencia alcalifaciens
- Authors: M. J. Albert, M. Ansaruzzaman, N. A. Bhuiyan, P. K. B. Neogi, A. S. G. Faruque
- J. Med. Microbiol., March 1995 42: 186-190, doi: 10.1099/00222615-42-3-186
- Subject: Models Of Infection
- Published Online:
Previous studies with three isolates from diarrhoeal stools suggested that Providencia alcalifaciens is an invasive enteric pathogen that also causes actin condensation in infected cells. These findings were extended in the present study with a further 14 diarrhoeal stool isolates of P. alcalifaciens and HEp-2 cell monolayers for invasion assays. Studies on invasion characteristics with two selected isolates suggested that P. alcalifaciens required prior growth at 37°C for better invasion. Invasion and actin condensation were inhibited by an agent that inhibits microfilament formation, but not by agents that inhibit receptormediated endocytosis, microtubule formation, endosome acidification or receptor recycling. In time-course assays with HEp-2 cell monolayers maintained in medium containing gentamicin, P. alcalifaciens showed a small degree of multiplication after invasion of the cells, but viable bacteria could not be recovered over a 24-h period although the integrity of the cell monolayer was preserved during this period.
© 1995 Pathological Society of Great Britain and Ireland | Published by the Microbiology Society
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