1887

Abstract

Surmmary

Cytokine production was measured in mice during sepsis and intoxication. In mice given live (10 cfu/mouse), by intra-peritoneal injection, serum levels of tumour necrosis factor (TNF)- and interleukin-6 increased steadily from day 1 until day 4. Interferon- levels showed a transient peak on day 3. Interleukin-1- levels were very low. There were high bacterial counts in the livers at day 3 and deaths occurred from day 4 onwards. Intraperitoneal injection of lipopolysaccharide or heat-killed bacteria also induced all of the cytokines, but their time of appearance and levels varied greatly. Cytokine induction by heat-killed bacteria was more marked. Endotoxaemia decreased with time during intoxication and increased during sepsis. Bioactive TNF, as measured by a cytotoxicity assay, was found only in mice given heat-killed bacteria.

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/content/journal/jmm/10.1099/00222615-42-5-348
1995-05-01
2024-03-28
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References

  1. Plant J., Glynn A. A. Locating Salmonella resistance gene on mouse chromosome 1. Clin Exp Immunol 1979; 37:1–6
    [Google Scholar]
  2. Freudenberg M. A., Kumazawa Y., Meding S., Langhorne J., Galanos C. Gamma interferon production in endotoxin-responder and-nonresponder mice during infection. Infect Immun 1991; 59:3484–3491
    [Google Scholar]
  3. Peel J. E., Voirol M.-J., Kolly C., Gobet D., Martinod S. Induction of circulating tumor necrosis factor cannot be demonstrated during septicemic salmonellosis in calves. Infect Immun 1990; 58:439–442
    [Google Scholar]
  4. Kumazawa Y., Freudenberg M. A., Hausmann C., Meding-Slade S., Langhorne J., Galanos C. Formation of interferon-gamma and tumor necrosis factor in mice during Salmonella typhimurium infection. Pathobiology 1991; 59:194–196
    [Google Scholar]
  5. Matsuura M., Galanos C. Induction of hypersensitivity to endotoxin and tumor necrosis factor by sublethal infection with Salmonella typhimurium . Infect Immun 1990; 58:935–937
    [Google Scholar]
  6. Fisch H., Gifford G. E. A photometric and plaque assay for macrophage mediated tumor cell cytotoxicity. J Immunol Methods 1983; 57:311–325
    [Google Scholar]
  7. Cross A. S., Opal S. M., Sadoff J. C., Gemski P. Choice of bacteria in animal models of sepsis. Infect Immun 1993; 61:2741–2747
    [Google Scholar]
  8. Ramarathinam L., Niesel D. W., Klimpel G. R. Ity influences the production of IFN-gamma by murine splenocytes stimulated in vitro with Salmonella typhimurium . J Immunol 1993; 150:3965–3972
    [Google Scholar]
  9. Nauciel C., Espinasse-Mase F. Role of gamma interferon and tumor necrosis factor alpha in resistance to Salmonella typhimurium infection. Infect Immun 1992; 60:450–454
    [Google Scholar]
  10. Nakano Y., Onozuka K., Terada Y., Shinomiya H., Nakano M. Protective effect of recombinant tumor necrosis factor-alpha in murine salmonellosis. J Immunol 1990; 144:1935–1941
    [Google Scholar]
  11. Flad H. D., Loppnow H., Rietschel E. T., Ulmer A. J. Agonists and antagonists for lipopolysaccharide-induced cytokines. Immunobiology 1993; 187:303–316
    [Google Scholar]
  12. Luo G., Niesel D. W., Shaban R. A., Grimm E. A., Klimpel G. R. Tumor necrosis factor alpha binding to bacteria: evidence for a high-affinity receptor and alteration of bacterial virulence properties. Infect Immun 1993; 61:830–835
    [Google Scholar]
  13. Cross A. S., Sadoff J. C., Kelly N., Bernton E., Gemski P. Pre treatment with recombinant murine tumor necrosis factor alpha/cachectin and murine interleukin 1 alpha protects mice from lethal bacterial infection. J Exp Med 1989; 169:2021–2027
    [Google Scholar]
  14. Fong Y., Tracey K. J., Moldawar L. L. Antibodies to cachectin/tumor necrosis factor reduce interleukin-1 beta and interleukin-6 appearance during lethal bacteremia. J Exp Med 1989; 170:1627–1633
    [Google Scholar]
  15. Nakano M., Onozuka K., Yamasu H., Zhong W. F., Nakano Y. Protective effects of cytokines in murine Salmonella . Adv Exp Med Biol 1992; 319:89–95
    [Google Scholar]
  16. Weinbaum G. (ed) Bacterial endotoxins. Microbial toxins vol 4 New York: Academic Press Inc; 1971
    [Google Scholar]
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