%0 Journal Article %A Rüssmann, H. %A Kothe, E. %A Schmidt, H. %A Franke, S. %A Harmsen, D. %A Caprioli, A. %A Karch, H. %T Genotyping of Shiga-like toxin genes in non-O157 Escherichia coli strains associated with haemolytic uraemic syndrome %D 1995 %J Journal of Medical Microbiology, %V 42 %N 6 %P 404-410 %@ 1473-5644 %R https://doi.org/10.1099/00222615-42-6-404 %I Microbiology Society, %X Surmmary The pheno- and genotypes of Shiga-like toxins (SLTs) in non-O157 Escherichia coli strains from patients with haemolytic uraemic syndrome were determined. The clinical isolates investigated were from Italy and Germany and belonged to serotypes O22:H8, O26:H−, O26:H11, O91:H−, O111:H− and O128:H−; one isolate was non-typable. SLT genotypes were analysed by complete nucleotide sequence analysis of the B-subunit genes. The results showed that 14 strains possessed slt-I alone, two contained slt-II alone and five isolates harboured both slt-I and slt-II genes. In only two strains were slt-II-related genes found, together with either slt-I or slt-II. These findings indicate that variants of SLT-II are rarely found in non-O157 E. coli isolates from patients with haemolytic uraemic syndrome. Polymerase chain reaction (PCR) with Taq cycle sequencing was found to be a suitable method for classification of slt genotypes. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-42-6-404