1887

Journal of Medical Microbiology logo

Volume 43, Issue 5

Efficacy of sustained release ciprofloxacin microspheres against device-associated biofilm infection in a rabbit peritoneal model Free

Abstract

Summary

The relative effectiveness of a poly(L-lactic acid) ciprofloxacin hydrochloride (CIP) microsphere formulation (250–425 μm) against peritoneal implanted biofilm of was investigated in a rabbit model. Correlations between in-vivo CIP pharmacokinetics in peritoneal dialysate and serum after intraperitoneal administration, in-vivo cell counts and rabbit survival rate were obtained. Dialysate and serum concentrations after 12 h (C) were greater than those obtained with free drug whereas maximum serum concentrations (C) were lower and the time to reach C (t) was longer. A silastic implant device pre-colonised with for 2 days was implanted in the rabbit peritoneum, and dialysate with or without drug or microspheres was administered via a catheter. Rabbits receiving no antibiotic and those receiving free drug (10 mg in dialysate) died of peritonitis and septicaemia, whereas all rabbits given CIP microspheres recovered completely from infection. The viable count of was markedly reduced or eliminated from the catheter, the device and the peritoneal wall in CIP microsphere-treated rabbits but not in rabbits treated with free drug, as determined from histological and scanning electronmicroscopic evidence. These results demonstrate that sustained release of antibiotics at biofilm eradication concentrations (BEC) is required to treat biofilm infections associated with peritoneal implanted devices.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1995
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-43-5-368
1995-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/43/5/medmicro-43-5-368.html?itemId=/content/journal/jmm/10.1099/00222615-43-5-368&mimeType=html&fmt=ahah

References

  1. Costerton J. W., Cheng K.-J., Geesey G. G. Bacterial biofilms in nature and disease. Amu Rev Microbiol 1987; 41:435–464
     [Google Scholar]
  2. Gristina A. G. Biomaterial-centred infection: microbial adhesion versus tissue integration. Science 1987; 237:1588–1595
     [Google Scholar]
  3. Dougherty S. H. Pathology of infection in prosthetic devices. Rev Infect Dis 1988; 10:1102–1117
     [Google Scholar]
  4. Sells C. J., Shurtleff D. B., Loeser J. D. Gram-negative cerebrospinal fluid shunt-associated infections. Pediatrics 1977; 59:614–618
     [Google Scholar]
  5. Costerton J. W., Lam J., Lam K., Chan R. The role of the microcolony mode of growth in the pathogenesis of Pseudomonas aeruginosa infections. Rev Infect Dis 1983; 5: Suppl 5S867–S873
     [Google Scholar]
  6. Kunin C. M., Steele C. Culture of the surfaces of urinary catheters to sample urethral flora and study the effect of antimicrobial therapy. J Clin Microbiol 1985; 21:902–908
     [Google Scholar]
  7. Hanker J. S., Giammara B. L. Biomaterials and biomedical devices. Science 1988; 242:885–892
     [Google Scholar]
  8. Anwar H., Strap J. L., Costerton J. W. Susceptibility of biofilm cells of Pseudomonas aeruginosa to bactericidal actions of whole blood and serum. FEMS Microbiol Lett 1992; 92:235–242
     [Google Scholar]
  9. Anwar H., Costerton J. W. Enhanced activity of combination of tobramycin and piperacillin for eradication of sessile biofilm cells of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1990; 34:1666–1671
     [Google Scholar]
  10. Anwar H., Dasgupta M. K., Costerton J. W. Testing the sus ceptibility of bacteria in biofilms to antibacterial agents. Antimicrob Agents Chemother 1990; 34:2043–2046
     [Google Scholar]
  11. Anwar H., Strap J. L., Costerton J. W. Establishment of aging biofilms: possible mechanism of bacterial resistance to antimicrobial therapy. Antimicrob Agents Chemother 1992; 36:1347–1351
     [Google Scholar]
  12. Mion C., Slingeneyer A., Canaud B. Peritonitis. In Gokal R. (ed) Continuous ambulatory peritoneal dialysis Edinburgh: Churchill Livingstone; 1986163–217
     [Google Scholar]
  13. Stephen IV. Peritonitis. In Nolph K. D. (ed) Peritoneal dialysis 3rd edn Dordrecht: Kluwer Academic Publishers; 1989261–288
     [Google Scholar]
  14. Marrie T. J., Noble M. A., Costerton J. W. Examination of the morphology of bacteria adhering to peritoneal dialysis catheters by scanning and transmission electron microscopy. J Clin Microbiol 1983; 18:1388–1398
     [Google Scholar]
  15. Holmes C. J., Evans R. Biofilm and foreign body infections—the significance to CAPD associated peritonitis. Peritoneal Dialysis Bull 1986; 6:168–177
     [Google Scholar]
  16. Peterson P. K., Matzke G., Keane W. F. Current concepts in the management of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis. Rev Infect Dis 1987; 9:604–612
     [Google Scholar]
  17. MacDonald W. A., Watts J., Bowmer M. I. Factors affecting Staphylococcus epidermidis growth in peritoneal dialysis solutions. J Clin Microbiol 1986; 24:104–107
     [Google Scholar]
  18. Sheth N. K., Bartell C. A., Roth D. A. In vitro study of bacterial growth in continuous ambulatory peritoneal dialysis fluids. J Clin Microbiol 1986; 23:1096–1098
     [Google Scholar]
  19. Verbrugh H. A., Keane W. F., Conroy W. E., Peterson P. K. Bacterial growth and killing in chronic ambulatory peritoneal dialysis fluids. J Clin Microbiol 1984; 20:199–203
     [Google Scholar]
  20. de Paepe M., Lameire N., Belpaire F., Bogaert M. Peritoneal pharmacokinetics of gentamicin in man. Clin Nephrol 1983; 19:107–109
     [Google Scholar]
  21. Friedland J. S., Iveson T. J., Fraise A. P., Winearts C. G., Selkon J. B., Oliver D. O. A comparison between intraperitoneal ciprofloxacin and intraperitoneal vancomycin and gentamycin in the treatment of peritonitis associated with continuous ambulatory peritoneal dialysis (CAPD). J Antimicrob Chemother 1990; 26: Suppl. F 77–81
     [Google Scholar]
  22. Ludlam H. A., Barton I., White L., McMullin C., King A., Phillips I. Intraperitoneal ciprofloxacin for the treatment of peritonitis in patients receiving continuous ambulatory peritoneal dialysis (CAPD). J Antimicrob Chemother 1990; 25:843–851
     [Google Scholar]
  23. Dryden M. S., Wing A. J., Phillips I. Low dose intraperitoneal ciprofloxacin for the treatment of peritonitis in patients receiving continuous ambulatory peritoneal dialysis (CAPD). J Antimicrob Chemother 1991; 28:131–139
     [Google Scholar]
  24. Kampf D., Bomer K., Hain H., Conrad W. Multiple-dose-kinetics of ofloxacin after intraperitoneal application in CAPD patients. Perit Dial Int 1991; 11:317–321
     [Google Scholar]
  25. Rogers J. A., Owusu-Ababio G. Formulation of antibiotics in polymeric microcapsules. II. Ciprofloxacin. Proc Int Symp Control Rel Bioact Mater 1993; 20:354–355
     [Google Scholar]
  26. Owusu-Ababio G. Sustained release antibiotic microspheres for eradication of bacterial biofilm PhD Thesis University of Alberta; Edmonton, Alberta, Canada: 1994
     [Google Scholar]
  27. Marc L. Ciprofloxacin: chemistry, mechanism of action, resistance, antimicrobial spectrum, pharmacokinetics, clinical trials, and adverse reactions. Pharmacotherapy 1988; 8:3–33
     [Google Scholar]
  28. Ward K. H., Olson M. E., Lam K., Costerton J. W. Mechanism of persistent infection associated with peritoneal implants. J Med Microbiol 1992; 36:406–413
     [Google Scholar]
  29. Nickel J. C., Grant S. K., Lam K., Olson M. E., Costerton J. W. Bacteriologically stressed animal model of a new closed catheter drainage system with microbicidal outlet tube. Urology 1991; 38:280–289
     [Google Scholar]
  30. Bassey C. M., Baltch A. L., Smith R. P. Comparative antimicrobial activity of enoxacin, ciprofloxacin, amifloxacin, non-floxacin and ofloxacin against 177 bacterial isolates. J Antimicrob Chemother 1986; 17:623–628
     [Google Scholar]
  31. Nickel J. C., Ruseska I., Wright J. B., Costerton J. W. Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob Agents Chemother 1985; 27:619–624
     [Google Scholar]
  32. Nicholov R., Khoury A. E., Bruce A. W., DiCosmo D. Interaction of ciprofloxacin loaded liposomes with Pseudomonas aeruginosa cells. Cells Materials 1993; 3:321–326
     [Google Scholar]
  33. Brady J. M., Cutright D. E., Miller R. A., Battistone G. C. Resorption rate, route of elimination, and ultrastructure of implant site of polylactic acid in the abdominal wall of the rat. J Biomed Mater Res 1973; 7:155–166
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-43-5-368
Loading
Efficacy of sustained release ciprofloxacin microspheres against device-associated Pseudomonas aeruginosa biofilm infection in a rabbit peritoneal model
J. Med. Microbiol. 43, 368 (1995); https://doi.org/10.1099/00222615-43-5-368
/content/journal/jmm/10.1099/00222615-43-5-368
/content/journal/jmm/10.1099/00222615-43-5-368
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error