Molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis isolates
Liebisch, Babett and Schwarz, S.,, 44, 52-59 (1996),
doi = https://doi.org/10.1099/00222615-44-1-52,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= A collection of 31 epidemiologically unrelated Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) isolates obtained during a 12-year period was characterised by different molecular typing methods. Plasmid profile analysis, the detection of plasmid-encoded virulence genes and ribotyping allowed little or no further differentiation amongst these isolates. Two different hybridisation patterns were observed by IS200-typing of the S. Enteritidis isolates. However, pulsed-field gel electrophoretic separation of restriction endonuclease-digested whole-cell DNA provided a high level of discrimination amongst the 31 S. Enteritidis isolates. This could be increased by the comparative use of the three suitable restriction endonucleases XbaI, SpeI and NotI. Thus, pulsed-field gel electrophoresis proved to be superior in its discriminatory value to other molecular methods such as plasmid analysis, ribotyping or IS200-typing and represents a most helpful tool for the epidemiological typing of S. Enteritidis isolates.,
language=,
type=