1887

Abstract

colonises contact lenses during wear, although the frequency of isolation is generally low (0.6% contamination rate). A method for typing the colonising the eye or contact lens was developed, based upon ribotyping, serotyping and biotyping. Twelve different types of were isolated from the eyes, contact lenses, contact lens cases and fingers of contact lens wearers in the Sydney area over a 2-year period. There was no evidence of a specific type being more readily able to colonise the contact lenses than other types. Indeed, eight strains were isolated from the lenses and these belonged to seven types. The diversity of types isolated from the eye indicates that there is probably not a subset of that can colonise the eye, although the results suggest that the types of strains isolated from contact lenses are different from those isolated from nosocomial infections.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-2-127
1996-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/2/medmicro-45-2-127.html?itemId=/content/journal/jmm/10.1099/00222615-45-2-127&mimeType=html&fmt=ahah

References

  1. Von Graevenitz A. Human disease due to Serratia. In Von Graevenitz A., Rubin S. J. (eds) The genus Serratia Boca Raton: Florida, CRC Press; 198067–186
    [Google Scholar]
  2. Atlee W. E., Bums R. P., Oden M. Serratia marcescens keratoconjunctivitis. Am J Ophthalmol 1970; 70:31–33
    [Google Scholar]
  3. Salceda S. R., Lapuz J., Vizconde R. Serratia marcescens endophthalmitis. Arch Ophthalmol 1973; 89:163–166
    [Google Scholar]
  4. Lass J. H., Haaf J., Foster C. S., Belcher C. Visual outcome in eight cases of Serratia marcescens keratitis. Am J Opthalmol 1981; 92:384–390
    [Google Scholar]
  5. Holden B. A., La Hood D., Grant T. Gram-negative bacteria can induce contact lens-related acute red eye (CLARE) responses. CLAO J (in press)
    [Google Scholar]
  6. Grant T., Terry R., Holden B. A. Extended wear of hydrogel lenses: clinical problems and their management. In Harris M. G. (ed) Problems in optometry Philadelphia: JB Lippincott; 1990599–622
    [Google Scholar]
  7. Grimont P. A. D., Grimon F. Biotyping of Serratia marcescens and its use in epidemiological studies. J Clin Microbiol 1978; 8:73–83
    [Google Scholar]
  8. Rubin S. J., Brock S., Chamberland M., Lyons R. W. Combined serotyping and biotyping of Serratia marcescens. J Clin Microbiol 1976; 3:582–585
    [Google Scholar]
  9. Larose P., Picard B., Thibault M., Grimont F., Goullet P. Nosocomial Serratia marcescens individualized by five typing methods in a regional hospital. J Hosp Infect 1990; 15:167–172
    [Google Scholar]
  10. Pitt T. L., Erdman Y. J., Bucher C. The epidemiological type identification of Serratia marcescens from outbreaks of infection in hospitals. J Hyg 1980; 84:269–283
    [Google Scholar]
  11. Bale M., Sanford M., Hollis R., Pfaller M. A. Application of a biotyping system and DNA restriction fragment analysis to the study of Serratia marcescens from hospitalized patients. Diagn Microbiol Infect Dis 1993; 16:1–7
    [Google Scholar]
  12. Sifuentes-Osomio J., Ruiz-Palacios G. M., Gröschel D. H. M. Analysis of epidemiologic markers of nosocomial Serratia marcescens isolates with special reference to the Grimont biotyping system. J Clin Microbiol 1986; 23:230–234
    [Google Scholar]
  13. McGeer A., Low D. E., Penner J., Ng J., Goldman C., Simor A. E. Use of molecular typing to study the epidemiology of Serratia marcescens. J Clin Microbiol 1990; 28:55–58
    [Google Scholar]
  14. Gargallo-Viola D. Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens. J Clin Microbiol 1989; 27:860–868
    [Google Scholar]
  15. Simor A. E., Ramage L., Wilcox L., Bull S. B., Bialkowska-Hobrzanska H. Molecular and epidemiologic study of multi-resistant Serratia marcescens infections in a spinal cord injury rehabilitation unit. Infect Control 1988; 9:20–27
    [Google Scholar]
  16. John J. F., McNeill W. F. Characteristics of Serratia marcescens containing a plasmid coding for gentamicin resistance in nosocomial infections. J Infect Dis 1981; 143:810–817
    [Google Scholar]
  17. Bingen E. H., Mariani-Kurkdjian P., Lambert-Zechovsky N. Y. Ribotyping provides efficient differentiation of nosocomial Serratia marcescens isolates in a pediatric hospital. J Clin Microbiol 1992; 30:2088–2091
    [Google Scholar]
  18. Alonso R., Aucken H. M., Perez-Diaz J. C., Cookson, BD., Baquero F., Pitt T. L. Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens. Epidemiol Infect 1993; 111:99–107
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning; a laboratory manual. 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989
    [Google Scholar]
  20. Davis J. T., Foltz E., Blakemore W. S. Serratia marcescens. A pathogen of increasing importance. JAMA 1970; 214:2190–2192
    [Google Scholar]
  21. Alexander S., Pinck B. D., Petzall G. Serratia marcescens infections. J Med Soc New Jersey 1970; 67:261–263
    [Google Scholar]
  22. Gaston M. A., Pitt T. L. C-antigen specificities of the serotype strains of Serratia marcescens. J Clin Microbiol 1989; 27:2697–2701
    [Google Scholar]
  23. Anagnostakis D., Fitsialos J., Koutsia C., Messaritakis J., Matsaniotis N. A. nursery outbreak of Serratia marcescens infection. Evidence of a single source of contamination. Am J Dis Child 1981; 135:413–414
    [Google Scholar]
  24. Maki D. G., Hennekens C. G., Phillips C. W., Shaw W. V., Bennett J. V. Nosocomial urinary tract infection with Serratia marcescens: an epidemiological study. J Infect Dis 1973; 128:579–587
    [Google Scholar]
  25. Yu V. L. Serratia marcescens. Historical perspective and clinical review. N Engl J Med 1979; 300:887–893
    [Google Scholar]
  26. Mayo M. S., Cook W. L., Schlitzer R. L., Ward M. A., Wilson L. A., Ahearn D. G. Antibiograms, serotypes, and plasmid profiles of Pseudomonas aeruginosa associated with corneal ulcers and contact lens wear. J Clin Microbiol 1986; 24:372–376
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-2-127
Loading
/content/journal/jmm/10.1099/00222615-45-2-127
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error