1887

Abstract

Isolates from three different populations were examined for restriction fragment length polymorphisms (RFLPs) of total DNA digested with the endonucleasc I. The populations were: community isolates collected at random from healthy individuals (38 isolates): methicillin-resistant (MRSA) type strains involved in separate outbreaks of infection in Melbourne (1982) and Canberra (1990) (two isolates); and a collection of clinical methicillin-sensitive (MSSA) causing hospital infection (20 isolates). RFLPs with I and I and hybridisation analyses of both, showed that the community and the MSSA isolates were not genetically closely related, and, accordingly, they could not be grouped into clusters as seen with the MRSA types. However, a few MSSA isolates were found to be closely related to each other and appeared to be similar to the standard strain 8325–4 and to some MRSA types. Although there was substantial variability between the three groups, physical mapping with genomic DNA fragments from the standard strain 8325-4 to probe large fragments generated with I and I from the chromosomes of selected community and MRSA isolates, demonstrated a well conserved genome organisation between representative isolates from the three groups.

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1997-04-01
2024-03-29
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References

  1. Musser J. M., Schlievert P. M., Chow A. W. A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. Proc Natl Acad Sci USA 1990; 87:225–229
    [Google Scholar]
  2. Musser J. M., Selander R. K. Genetic analysis of natural populations of Staphylococcus aureus . In Novick R. P. (ed) Molecular biology of the Staphylococci New York: VCH Publishers; 199059–67
    [Google Scholar]
  3. Musser J. M., Kapur V. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J Clin Microbiol 1992; 30:2058–2063
    [Google Scholar]
  4. El-Adhami W., Roberts L., Vickery A., Inglis B., Gibbs A., Stewart P. R. Epidemiological analysis of a methicillin-resistant Staphylococcus aureus outbreak using restriction fragment length polymorphisms of genomic DNA. J Gen Microbiol 1991; 137:2713–2720
    [Google Scholar]
  5. Stewart P. R., El-Adhami W., Inglis B., Franklin J. C. Analysis of an outbreak of variably methicillin-resistant Staphylococcus aureus with chromosomal RFLPs and mec region probes. J Med Microbiol 1993; 38:270–277
    [Google Scholar]
  6. Krawiec S., Riley M. Organization of the bacterial chromosome. Microbiol Rev 1990; 54:502–539
    [Google Scholar]
  7. Maynard Smith J., Smith N. H., O’Rourke M., Spratt B. G. How clonal are bacteria?. Proc Natl Acad Sci USA 1993; 90:4384–4388
    [Google Scholar]
  8. Milkman R., Bridges M. M. Molecular evolution of the Escherichia coli chromosome. III. Clonal frames. Genetics 1990; 126:505–517
    [Google Scholar]
  9. Maynard Smith J. The evolution of prokaryotes: does sex matter?. Ann Rev Ecol System 1990; 21:1–12
    [Google Scholar]
  10. Dykhuizen D. E., Green L. Recombination in Escherichia coli and the definition of biological species. J Bacteriol 1991; 173:7257–7268
    [Google Scholar]
  11. Maynard Smith J., Dowson C. G., Spratt B. G. Localized sex in bacteria. Nature 1991; 349:29–31
    [Google Scholar]
  12. Guttman D. S., Dykhuizen D. E. Detecting selective sweeps in naturally occurring Escherichia coli . Genetics 1994; 138:993–1003
    [Google Scholar]
  13. Guttman D. S., Dykhuizen D. E. Clonal divergence in Escherichia coli as a result of recombination, not mutation. Science 1994; 266:1380–1383
    [Google Scholar]
  14. Whatmore A. M., Kapur V., Sullivan D. J., Musser J. M., Kehoe M. A. Non-congruent relationships between variation in emm gene sequences and the population genetic structure of group A streptococci. Mol Microbiol 1994; 14:619–631
    [Google Scholar]
  15. Whatmore A. M., Kapur V., Musser J. M., Kehoe M. A. Molecular population genetic analysis of the enn subdivision of group A streptococcal emm-like genes: horizontal gene transfer and restricted variation among enn genes. Mol Microbiol 1995; 15:1039–1048
    [Google Scholar]
  16. Reda K. B., Kapur V., Goela D., Lamphear J. G., Musser J. M., Rich R. R. Phylogenetic distribution of streptococcal superantigen SSA allelic variants provides evidence for horizontal transfer of SSA within Streptococcus pyogenes . Infect Immun 1996; 64:1161–1165
    [Google Scholar]
  17. Patel A. H., Foster T. J., Pattee P. A. Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4. J Gen Microbiol 1989; 135:1799–1807
    [Google Scholar]
  18. Heneine N., Stewart P. R. Physiological determination of methicillin resistance in Staphylococcus aureus: comparison of clinical and genetically derived isolates. J Antimicrob Chemother 1986; 17:705–715
    [Google Scholar]
  19. Gillespie M. T., Lyon B. R., Loo L. S.L., Matthews P. R., Stewart P. R., Skurray R. A. Homologous direct repeat sequences associated with mercury, methicillin, tetracycline and trimethoprim resistance determinants in Staphylococcus aureus . FEMS Microbiol Lett 1987; 43:165–171
    [Google Scholar]
  20. Matthews P. R., Reed K. C., Stewart P. R. The cloning of chromosomal DNA associated with methicillin and other resistances in Staphylococcus aureus . J Gen Microbiol 1987; 133:1919–1929
    [Google Scholar]
  21. Skinner S., Inglis B., Matthews P. R., Stewart P. R. Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus . Mol Microbiol 1988; 2:289–292
    [Google Scholar]
  22. Inglis B., Matthews P. R., Stewart P. R. Induced deletions within a cluster of resistance genes in the mec region of the chromosome of Staphylococcus aureus . J Gen Microbiol 1990; 136:2231–2239
    [Google Scholar]
  23. Dubin D. T., Chikramane S. G., Inglis B., Matthews P. R., Stewart P. R. Physical mapping of the mec region of an Australian methicillin-resistant Staphylococcus aureus lineage and a closely related American strain. J Gen Microbiol 1992; 138:169–180
    [Google Scholar]
  24. Stewart P. R., El-Adhami W., Inglis B. Pulsed-field gel electrophoresis: applications to bacteria. In Dale J. W., Sanders P. G. Methods in gene technology London: JAI Press; 1994177–205
    [Google Scholar]
  25. Pattee P. The genetic map of Staphylococcus aureus . In Sonenshein A. L., Hoch J. A., Losick R. (eds) Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics Washington, DC: American Society for Microbiology; 1993489–496
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. (eds) Molecular cloning. A laboratory manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989
    [Google Scholar]
  27. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132:6–13
    [Google Scholar]
  28. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. ‘Addendum’. Anal Biochem 1984; 137:266–267
    [Google Scholar]
  29. Upholt W. B. Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 1977; 4:1257–1265
    [Google Scholar]
  30. Nei M., Li W.-H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76:5269–5273
    [Google Scholar]
  31. Pattee P. A. Genetic and physical mapping of the chromosome of Staphylococcus aureus NCTC 8325. In Drlica K., Riley M. (eds) The bacterial chromosome Washington, DC: American Society for Microbiology; 1990163–169
    [Google Scholar]
  32. Lyon B. R., Skurray R. A. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 1987; 51:88–134
    [Google Scholar]
  33. Pattee P. A., Lee H. C., Bannantine J. P. Genetic and physical mapping of the chromosome of Staphylococcus aureus . In Novick R. P. (ed) Molecular biology of the staphylococci New York: VCH Publishers; 199041–58
    [Google Scholar]
  34. Clewell D. B., Flannagan S. E., Ike Y., Jones J. M., Gawron-Burke C. Sequence analysis of termini of conjugative transposon Tn 916 . J Bacteriol 1988; 170:3046–3052
    [Google Scholar]
  35. Kuhl S. A., Pattee P. A., Baldwin J. N. Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus . J Bacteriol 1978; 135:460–465
    [Google Scholar]
  36. Weinstock G. M. Bacterial genomes: mapping and stability. ASM News 1994; 60:73–78
    [Google Scholar]
  37. Stewart P. R., Dubin D. T., Chikramane S. G., Inglis B., Matthews P. R., Poston S. M. IS257 and small plasmid insertions in the mec region of the chromosome of Staphylococcus aureus . Plasmid 1994; 31:12–20
    [Google Scholar]
  38. Byrne M. E., Gillespie M. T., Skurray R. A. 4′, 4" adenyltransferase activity on conjugative plasmids isolated from Staphylococcus aureus is encoded on an integrated copy of pUBllO. Plasmid 1991; 25:70–75
    [Google Scholar]
  39. Dubin D. T. Tn554 and related DNA: mapping and tracking the staphylococci chromosome. In Novick R. P. (ed) Molecular biology of the staphylococci New York: VCH Publishers; 199085–98
    [Google Scholar]
  40. Matthews P. R., Inglis B., Stewart P. R. Clustering of resistance genes in the mec region of the chromosomes of Staphylococcus aureus . In Novick R. P. (ed) Molecular biology of the staphylococci New York: VCH Publishers; 199069–83
    [Google Scholar]
  41. Green C. J., Void B. S. Staphylococcus aureus has clustered tRNA genes. J Bacteriol 1993; 175:5091–5096
    [Google Scholar]
  42. Cole S. T., Girons I. S. Bacterial genomics. FEMS Microbiol Rev 1994; 14:139–160
    [Google Scholar]
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