1887

Abstract

SUMMARY

A previous study described the isolation of multiply-resistant strains of from the gastro-intestinal tract after the ingestion of a strain carrying an R factor (Rl) that confers resistance to ampicillin (A), chloram-phenicol (C), kanamycin (K), streptomycin (S) and sulphadimidine (Su). One such strain carried the R factor R174 (ACKSSuT)–which was in addition resistant to tetracycline (T)–and another carried the factor R157 (AST).

The minimum inhibitory concentrations of these antibiotics for strains, isogenic but for the R factors, were consistent with the formation of factor R174 by recombination of the AST and ACKSSu resistance determinants of the other two R factors. The factor Rl 74 determined a streptomycin-phosphorylase enzyme also present in factor R157, together with the strepto-mycin-spectinomycin resistance determinant of factor Rl, and all three R-factors shared the same type of ampicillin resistance. Factor R174 carried all the other antibiotic-resistance determinants (TCKSu) of the parent plasmids and is thus probably a recombinent of factor Rl and R157.

DNA-DNA hybridisation showed that factor R174 contained almost all of the DNA-base sequences of factors R157 and Rl. There was also approximately 50% homology between the latter two R factors which may account for the occurrence of recombination.

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1974-05-01
2024-05-02
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References

  1. Anderson J. D., Gillespie W. A., Richmond M. H. 1973a; Chemotherapy and antibiotic resistance transfer between Enterobacteria in the human gastro-intestinal tract. J. med. Microbiol 6:461
    [Google Scholar]
  2. Anderson J. D., Ingram L. C., Richmond M. H., Wiedemann B. 1973b; Studies on the nature of plasmids arising from conjugation in the human gastro-intestinal tract. J. med. Microbiol 6:475
    [Google Scholar]
  3. Benveniste R., Yamada T., Davies J. 1970; Enzymatic adenylylation of streptomycin and spectinomycin by R-factor-resistant Escherichia coli. Infect. Immun 1:109
    [Google Scholar]
  4. Chabbert Y. A., Scavizzi M. R., Witchitz J. L., Gerbaud G. R., Bouanchaud D. H. 1972; Incompatibility groups and the classification of fi resistance factors. J. Bad 112:666
    [Google Scholar]
  5. Clowes R. C. 1972; Molecular structure of bacterial plasmids. Bad. Rev 36:361
    [Google Scholar]
  6. Cohen S. N., Miller C. A. 1970; Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factor isolated from Proteus mirabilis and Escherichia coli. J. molec. Biol 50:671
    [Google Scholar]
  7. Datta N., Hedges R. W. 1971; Compatibility groups among fi R-factors. Nature, Lond 234:222
    [Google Scholar]
  8. Datta N., Kontomichalou P. 1965; Penicillinase synthesis controlled by infectious R factors in enterobacteriaceae. Nature, Lond 208:239
    [Google Scholar]
  9. Datta N., Richmond M. H. 1966; The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem. J 98:204
    [Google Scholar]
  10. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun 23:641
    [Google Scholar]
  11. Farrar W. E., Eidson M., Guerry P., Falkow S., Drusin L. M., Roberts R. B. 1972; Interbacterial transfer of an R-factor in the human intestine: in-vivo acquisition of R-factor-mediated kanamycin resistance by a multi-resistant strain of Shigella sonnei. J. infect. Dis 126:27
    [Google Scholar]
  12. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. 1972; Properties of an R-factor which originated in Pseudomonas aeruginosa 1822. J. Bad 110:529
    [Google Scholar]
  13. Ingram L. 1973; Deoxyribonucleic acid—deoxyribonucleic acid hybridization of R-factors. J. Bad 115:1130
    [Google Scholar]
  14. Ingram L., Sykes R. B., Grinsted J., Saunders J. R., Richmond M. H. 1972; A transmissible resistance element from a strain of Pseudomonas aeruginosa containing a detectable extrachromosomal DNA. J. gen. Microbiol 72:269
    [Google Scholar]
  15. Meynell E., Datta N. 1966; The relation of resistance transfer factors to the F factor (Sex factor) in Escherichia coli K12. Genet. Res 7:134
    [Google Scholar]
  16. Nisioka T., Mitani M., Clowes R. C. 1970; Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells. J. Bad 103:166
    [Google Scholar]
  17. Novick R. P. 1962; Staphylococcal penicillinase and the new penicillins. Biochem. J 83:229
    [Google Scholar]
  18. Ozanne B., Benveniste R., Tipper D., Davies J. 1969; Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R-factors. J. Bad 100:1144
    [Google Scholar]
  19. Perret C. J. 1954; Iodometric assay of penicillinase. Nature, Lond 174:1012
    [Google Scholar]
  20. Watanabe T., Fukasawa T. 1961; Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduction of resistance factors. J. Bad 82:202
    [Google Scholar]
  21. Yokota T., Kanamaru Y., Mori R., Akiba T. 1969; Recombination between a thermosensitive kanamycin resistance factor and nonthermosensitive multiple-drug resistance factor. J. Bad 98:863
    [Google Scholar]
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