1887

Abstract

SUMMARY

One hundred specimens of nasopharyngeal secretion were collected from infants with acute respiratory disease and stored at -60°C before inoculation into tube cultures of bovine testis (BT) and HeLa (HL) cells. Of a total of 46 specimens which yielded respiratory syncytial virus, 45 did so in BT cells but only 32 in HL cells. The mean incubation time before a cytopathic effect was first detected was 6.5 days for BT cells and 10.6 days for HL cells.

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1974-05-01
2024-05-02
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References

  1. Aitkin C. J. D., Moffat M. A. J., Sutherland J. A. W. 1967; Respiratory illness and viral infection in an Edinburgh nursery. J. Hyg., Camb 65:25
    [Google Scholar]
  2. Anderson J. M., Beem M. O. 1966; Use of human diploid cell cultures for primary isolation of respiratory syncytial virus. Proc. Soc. exp. Biol. Med 121:205
    [Google Scholar]
  3. Beem M. O. 1969; Acute respiratory illness in nursery school children: a longitudinal study of the occurrence of illness and respiratory viruses. Am. J. Epidem 90:30
    [Google Scholar]
  4. Beem M. O., Wright F. H., Hamre D., Egerer R., Oehme M. 1960; Association of chimpanzee coryza agent with acute respiratory disease in children. New Engl. J. Med 263:523
    [Google Scholar]
  5. Berglund B., Strahlmann C.-H. 1967; Respiratory syncytial virus infections in hospitalised children: evaluation of virus isolation and complement fixation techniques in die virological diagnosis: clinical and epidemiological characteristics. Acta paediat. scand 56:269
    [Google Scholar]
  6. Berglund B., Vihma L., Wickstrom J. 1965; Respiratory syncytial virus studies on children hospitalized during an outbreak of respiratory illness in Finland. Am. J. Epidem 81:271
    [Google Scholar]
  7. Clarke S. K. R., Corner B. D., Gambier D. M., Macrae J., Peacock D. B. 1964; Viruses associated with the acute respiratory infections. Br. med. J 1:1536
    [Google Scholar]
  8. Coates H. V., Forsyth B. R., Chanock R. M. 1966; Biophysical studies of respiratory syncytial virus. I. Density of respiratory syncytial virus and associated complementfixing antigens in a cesium chloride density gradient. J. Bact 91:1263
    [Google Scholar]
  9. Gharpure M. A., Wright P. F., Chanock R. M. 1969; Temperature-sensitive mutants of respiratory syncytial virus. J. Virol 3:414
    [Google Scholar]
  10. Hambling M. H. 1964; Survival of the respiratory syncytial virus during storage under various conditions. Br. J. exp. Path 45:647
    [Google Scholar]
  11. Holzel A., Parker L., Patterson W. H., Cartmel D., White L. L. R., Purdy R., Thompson K. M., Tobin J. O’H. 1965; Virus isolations from throats of children admitted to hospital with respiratory and other diseases, Manchester 1962-4. Br. med. J 1:614
    [Google Scholar]
  12. Jacobs J. W., Peacock D. B., Corner B. D., Caul E. O., Clarke S. K. R. 1971; Respiratory syncytial and other viruses associated with respiratory disease in infants. Lancet 1:871
    [Google Scholar]
  13. Law T. J., Hull R. N. 1968; The stabilizing effect of sucrose upon respiratory syncytial virus infectivity. Proc. Soc. exp. Biol. Med 128:515
    [Google Scholar]
  14. Peacock D. B., Clarke S. K. R. 1961; Respiratory syncytial virus in Britain. Lancet 2:466
    [Google Scholar]
  15. Stott E. J., Bell E. J., Eadee M. B., Ross C. A. C., Grist N. R. 1967; A comparative virological study of children in hospital with respiratory and diarrhoeal illnesses. J. Hyg., Camb 65:9
    [Google Scholar]
  16. Sturdy P., Mcquillin J., Gardner P. S. 1969; A comparative study of methods for the diagnosis of respiratory virus infections in childhood. J. Hyg., Camb 67:659
    [Google Scholar]
  17. Suto T., Yano N., Ikeda M., Miyamoto M., Takai S., Shigeta S., Hinuma Y., Ishida N. 1965; Respiratory syncytial virus infection and its serologic epidemiology. Am. J. Epidem 82:211
    [Google Scholar]
  18. Young B. J., Matthews R. S. 1969; A comparison of HEp-2 cells and a continuous line of human amnion cells for the primary isolation of respiratory syncytial virus. J. med. Lab. Technol 26:93
    [Google Scholar]
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