1887

Abstract

All of six reference strains of Bacteroides species, 36 laboratory isolates conforming to this group, and individual strains of Escherichia coli, Proteus mirabilis, Salmonella typhimurium and Clostridium welchii produced a dense black pigment, identified as ferrous sulphide, when grown in cooked-meat media containing cysteine and ferrous sulphate. This was an indicator effect resulting from the production of H2S by the bacteria in the presence of ferrous ions and was unrelated to the characteristic pigment produced by strains of B. melaninogenicus when grown on blood agar. A pigment was extracted by ultrasonic disintegration of washed cells of three reference strains of B. melaninogenicus grown for 1 week in horse-blood broth and on human-blood agar. It was intracellular or cell-associated, soluble in water and had the spectrophotometric characteristics of a derivative of haemoglobin. No such pigment was extracted from strains of B. fragilis or B. necrophorus by similar procedures. Pigment production is a stable characteristic of those strains of Bacteroides called B. nzelaninogenicus and it is a significant property in the classification of the Bacteroides group. However, the pigment-producing strains are not a homogeneous species, and there were considerable differences between the resuIts of biochemical tests and antibiograms obtained with the three strains of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-8-1-113
1975-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/8/1/medmicro-8-1-113.html?itemId=/content/journal/jmm/10.1099/00222615-8-1-113&mimeType=html&fmt=ahah

References

  1. Barnes E. M., Impey C. S. 1971; The isolation of the anaerobic bacteria from chicken caeca with particular reference to members of the family Bacteroidaceae. In Isolation of anaerobes (Soc. Appl. Bact. Tech. Series No. 5), edited by Shapton D. A., Board R. G. London and New York: p 118
    [Google Scholar]
  2. Beerens H. 1970; Report of the International Committee on Nomenclature of Bacteria Taxonomic Subcommittee for Gram-negative Anaerobic Rods. Int. J. syst. Bact 20:297
    [Google Scholar]
  3. Collee J. G., Watt B., Fowler E. B., Brown R. 1972; An evaluation of the Gaspak system in the culture of anaerobic bacteria. J. appl. Bact 35:71
    [Google Scholar]
  4. Cruickshank R. 1969 Medical microbiology 11th ed. (revised reprint) Edinburgh and London: p 757
    [Google Scholar]
  5. Finegold S. M., Harada N. E., Miller L. G. 1967; Antibiotic susceptibility patterns as aids in classification and characterisation of Gram-negative anaerobic bacilli. J. Bact 94:1443
    [Google Scholar]
  6. Gibbons R. J., MacDonald J. B. 1960; Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J. Bact 80:164
    [Google Scholar]
  7. Holdeman L. V., Moore W. E. C. 1972; Anaerobe laboratory manual. Blacksburg, Va p 27
    [Google Scholar]
  8. Lev M. 1959; The growth promoting activity of compounds of the vitamin K group and analogues for a rumen strain of Fusiformis nigrescens (Bact. melaninogenicum). J. gen. Microbiol 20:697
    [Google Scholar]
  9. Oliver W. W., Wherry W. B. 1921; Notes on some bacterial parasites of the human mucous membranes. J. infect. Dis 28:341
    [Google Scholar]
  10. Rutter J. M. 1970; A study of the carbohydrate fermentation reactions of Clostridium oedematiens (Cl. novyi). J. med. Microbiol 3:283
    [Google Scholar]
  11. Sawyer S. J., MacDonald J. B., Gibbons R. J. 1962; Biochemical characteristics of Bacteroides melaninogenicus. A study of thirty-one strains. Archs oral Biol 7:685
    [Google Scholar]
  12. Schwabacher H., Lucas D. R., Rimington C. 1947; Bacterium melaninogenicus—a misnomer. J. gen. Microbiol 1:109
    [Google Scholar]
  13. Senos G., Mattman L. H. 1955; Notes on Bacteroides melaninogenicus. J. Bact 70:483
    [Google Scholar]
  14. Sutter V. L., Finegold S. M. 1971; Antibiotic disc susceptibility testing for rapid presumptive identification of Gram-negative anaerobic bacilli. Appl. Microbiol 21:13
    [Google Scholar]
  15. Tracy O. 1969; Pigment production in Bacteroides. J. med. Microbiol 2:309
    [Google Scholar]
  16. Weiss C. 1937; Observations on Bacterium melaninogenicum: demonstration of fibrinolysin, pathogenicity and serological types. Proc. Soc. exp. Biol. Med 37:473
    [Google Scholar]
  17. Werner H., Pulverer G., Rjeichertz C. 1971; The biochemical properties and antibiotic susceptibility of Bacteroides melaninogenicus. Med. Microbiol Immunol., Berlin 157:3
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-8-1-113
Loading
/content/journal/jmm/10.1099/00222615-8-1-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error