1887

Abstract

SUMMARY

Faecal filtrates containing rotavirus particles, from children with acute infectious diarrhoea, were inoculated intranasally into gnotobiotic piglets. The piglets developed no symptoms, but virus was readily found by electron microscopy in their faeces during three serial passages. Among 11 piglets tested 3 weeks after inoculation of virus, all had developed fluorescent antibodies against tissue-culture-adapted calf rotavirus but only two had neutralising antibody. Growth of human rotavirus did not occur in either normal or “nude”, thymus-deficient suckling mice.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-8-4-565
1975-11-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/8/4/medmicro-8-4-565.html?itemId=/content/journal/jmm/10.1099/00222615-8-4-565&mimeType=html&fmt=ahah

References

  1. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1973; Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet 2:1281
    [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1974; Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet 1:149
    [Google Scholar]
  3. Bridger J. C., Woode G. N. 1975; Neonatal calf diarrhoea: identification of a reovirus-like agent (rotavirus) in faeces by immunofluorescence and immune electron microscopy. Br. vet. J 131: in the press
    [Google Scholar]
  4. Cruickshank J. G., Axton J. H. M., Webster O. F. 1974; Viruses in gastroenteritis. Lancet 2:1353
    [Google Scholar]
  5. Davidson G. P., Bishop R. F., Townley R. R. W., Holmes I. H., Ruck B. J. 1975; Importance of a new virus in acute sporadic enteritis in children. Lancet 2:242
    [Google Scholar]
  6. Els H. J., Lecatsas G. 1972; Morphological studies on simian virus S.A.ll and the “related” O agent. J. gen. Virol 17:129
    [Google Scholar]
  7. Flewett T. H., Bryden A. S., Davies H. 1973; Virus particles in gastroenteritis. Lancet 2:1497
    [Google Scholar]
  8. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. 1974a; Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet 2:61
    [Google Scholar]
  9. Flewett T. H., Davies H., Bryden A. S., Robertson M. J. 1974b; Diagnostic electron microscopy of faeces. II. Acute gastroenteritis associated with reovirus-like particles. J. clin. Path 27:608
    [Google Scholar]
  10. Holmes I. H., Mathan M., Bhat P., Albert M. J., Swaminathan S. P., Maiya P. P., Pereira S. M., Baker S. J. 1974; Orbiviruses and gastroenteritis. Lancet 2:658
    [Google Scholar]
  11. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. 1974; Reovirus-like agent in stools: association with infantile diarrhea and development of serologic tests. Science, N. Y 185:1049
    [Google Scholar]
  12. Mebus C. A., Underdahl N. R., Rhodes M. B., Twiehaus M. J. 1969; Calf diarrhea (scours): reproduced with a virus from a field outbreak. University of Nebraska Agriculture Experimental Station Research Bulletin no. 233
    [Google Scholar]
  13. Middleton P. J., Szymanski M. T., Abbott G. D., Bortolussi R., Hamilton J. R. 1974; Orbivirus acute gastroenteritis of infancy. Lancet 2:1241
    [Google Scholar]
  14. Much D. H., Zajac I. 1972; Purification and characterization of epizootic diarrhea of infant mice virus. Infect. Immun 6:1019
    [Google Scholar]
  15. Ørstavik I., Figenschau K. J., Ulstrup J. C. 1974; “Rotavirus” in stored specimens of faecal extracts. Lancet 2:1083
    [Google Scholar]
  16. Sexton M., Davidson G. P., Bishop R. F., Townley R. R. W., Holmes I. H., Ruck B. J. 1974; Viruses in gastroenteritis. Lancet 2:355
    [Google Scholar]
  17. Tan G. S., Townley R. R. W., Davidson G. P., Bishop R. F., Holmes I. H., Ruck B. J. 1974; Virus in faecal extracts from children with gastroenteritis. Lancet 1:1109
    [Google Scholar]
  18. Tavernor W. D., Trexler P. C., Vaughan L. C., Cox J. E., Jones D. G. C. 1971; The production of gnotobiotic piglets and calves by hysterotomy under general anaesthesia. Vet. Rec 88:10
    [Google Scholar]
  19. White G. B. B., Ashton C. I., Roberts C., Parry H. E. 1974; “Rotavirus” in gastroenteritis. Lancet 2:726
    [Google Scholar]
  20. Woode G. N., Bridger J. C. 1975; Viral enteritis of calves. Vet. Rec 96:85
    [Google Scholar]
  21. Woode G. N., Bridger J. C., Hall G., Dennis M. J. 1974; The isolation of a reovirus like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res. vet. Sci 16:102
    [Google Scholar]
  22. Wyatt R. G., Kapikian A. Z., Thornhill T. S., Sereno M. M., Kim H. W., Chanock R. M. 1974; In vitro cultivation in human fetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children. J. infect. Dis 130:523
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-8-4-565
Loading
/content/journal/jmm/10.1099/00222615-8-4-565
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error