1887

Abstract

Summary

Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-lethal quantity of meningococcal endotoxin, or with an equivalent dose of endotoxin in the form of meningococcal cell-wall blebs. Of 32 rabbits treated in this manner, 12 developed disseminated intravascular coagulation and six of these 12 had renal cortical necrosis. The remainder exhibited less severe lesions resembling those of endotoxaemia. Rabbits were not sensitised to meningococcal endotoxin when materials egested from leucocytes containing were used. A description of the pathological findings in the rabbits is presented.

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1976-11-01
2024-05-02
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References

  1. Cohn Z. A. 1963; The fate of bacteria within phagocytic cells. I. The degradation of isotopically labeled bacteria by polymorphonuclear leucocytes and macrophages. J. exp. Med 117:27
    [Google Scholar]
  2. Collins R. D., Wood W. B. 1959; Studies on the pathogenesis of fever. VI. The interaction of leucocytes and endotoxin in vitro. J. exp. Med 110:1005
    [Google Scholar]
  3. DeVoe I. W. 1976; Egestion of degraded meningococci by polymorphonuclear leucocytes. J. Bact 125:258
    [Google Scholar]
  4. DeVoe I. W., Gilchrist J. E. 1973; Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J. exp. Med 138:1156
    [Google Scholar]
  5. DeVoe I. W., Gilchrist J. E. 1975; Pili on meningococci from primary cultures of nasopharyngeal carriers and cerebrospinal fluid of patients with acute disease. J. exp. Med 141:297
    [Google Scholar]
  6. DeVoe I. W., Gilchrist J. E., Storm D. W. 1973; Ultrastructural studies on the fate of group B meningococci in human peripheral blood leucocytes. Can. J. Microbiol 19:1355
    [Google Scholar]
  7. DeVoe I. W., Storm D. W., Gilchrist J. E. 1973; A study of phagocytosis of radiolabeled Staphylococcus epidermidis and on structural events during intracellular degradation. Can. J. Microbiol 19:525
    [Google Scholar]
  8. Ducker T. B., Simmons R. L. 1968; The pathogenesis of meningitis: systemic effects of meningococcal endotoxin with the cerebrospinal fluid. Archs Neurol 18:123
    [Google Scholar]
  9. Ferguson J. H., Chapman O. D. 1948; Fulminating meningococcic infections and the so-called Waterhouse-Friderichsen syndrome. Am. J. Path 24:763
    [Google Scholar]
  10. Filkins J. P. 1971; Comparison of endotoxin detoxification by leucocytes and macrophages. Proc. Soc. exp. Biol. Med 137:1396
    [Google Scholar]
  11. Flexner S. 1907; Experimental cerebro-spinal meningitis in monkeys. J. exp. Med 9:142
    [Google Scholar]
  12. Hirsch J. G., Cohn Z. A. 1960; Degranulation of polymorphonuclear leucocytes following phagocytosis of microorganisms. J. exp. Med 112:1005
    [Google Scholar]
  13. Horn R. G. 1973; Evidence for participation of granulocytes in the pathogenesis of the generalized Shwartzman reaction: a review. J. infect. Dis 128:S134
    [Google Scholar]
  14. Leffel M. S., Spitznagel J. K. 1974; Intracellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes. Infect. Immun 10:1241
    [Google Scholar]
  15. Murray E. G. D. 1929 The meningococcus. Medical Research Council. Special Report Series no. 124 London:
    [Google Scholar]
  16. Osborn M. J. 1963; Studies on the gram-negative cell walls. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proc. natn Acad. Sci., U.S.A 50:499
    [Google Scholar]
  17. Roberts R. B. 1967; The interaction in vitro between group B meningococci and rabbit polymorphonuclear leucocytes. Demonstration of type specific opsonins and bacteri-cidens. J. exp. Med 126:795
    [Google Scholar]
  18. Scherp H. W. 1955; Neisseria and neisserial infections. A. Rev. Microbiol 9:319
    [Google Scholar]
  19. Smith H. 1968; Biochemical challenge of microbial pathogenicity. Bact. Rev 32:164
    [Google Scholar]
  20. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli. B. J. biol. Chem 234:705
    [Google Scholar]
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