1887

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Volume 64, Issue 2

Clonal diversity of CTX-M-producing, multidrug-resistant from rodents Free

Abstract

This territory-wide study investigated the occurrence of faecal carriage of extended-spectrum β-lactamase (ESBL)-producing among wild rodents from the 18 districts in Hong Kong. Individual rectal swabs were obtained from the trapped animals and cultured in plain and selective media. A total of 965 wild rodents [148 chestnut spiny rats (), 326 Indo-Chinese forest rats (), 452 brown rats () and 39 black rats ()] were sampled. ESBL carriage was 0 % in chestnut spiny rats, 0.6 % in Indo-Chinese forest rats, 7.7 % in black rats and 13.9 % in brown rats. Among brown rats, the prevalence of ESBL carriage differed markedly by geographical location: absent in two districts, low (7–10 %) in six districts, moderate (11–19 %) in seven districts and high (21–50 %) in three districts. Nonetheless, there was no correlation between the prevalence of ESBL in brown rats and human population density in the 18 districts. CTX-M-type enzymes were detected in 92.0 % of the ESBL-producing isolates, of which 83.1 % were resistant to three or more non-β-lactam drugs. The CTX-M producing isolates were genetically diverse but a large proportion (47.8 %) were included in six successful clones that are strongly associated with human diseases and CTX-M dissemination, viz. sequence type complex [STC]10/phylogroup A, STC23/phylogroup B1, STC38/phylogroup D, STC155/phylogroup B1, ST405/phylogroup D and ST131/phylogroup B2. In conclusion, our results show that brown rats often carry potentially zoonotic clones of CTX-M producing, multidrug-resistant . The potential for rats to be a source of CTX-M producing for humans deserves further consideration.

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© 2015 The Authors

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2015-02-01
2024-05-03
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References

  1. Banerjee R., Robicsek A., Kuskowski M. A., Porter S., Johnston B. D., Sokurenko E., Tchesnokova V., Price L. B., Johnson J. R. 2013; Molecular epidemiology of Escherichia coli sequence type 131 and Its H30 and H30-Rx subclones among extended-spectrum-β-lactamase-positive and -negative E. coli clinical isolates from the Chicago Region, 2007 to 2010. Antimicrob Agents Chemother 57:6385–6388 [View Article][PubMed]
     [Google Scholar]
  2. Clermont O., Christenson J. K., Denamur E., Gordon D. M. 2013; The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ Microbiol Rep 5:58–65 [View Article][PubMed]
     [Google Scholar]
  3. CLSI 2013; Performance Standards for Antimicrobial Susceptibility Testing. , 23rd. Informational Supplement M100-S23 Wayne, PA: Clinical and Laboratory Standards Institute;
     [Google Scholar]
  4. Collignon P., Powers J. H., Chiller T. M., Aidara-Kane A., Aarestrup F. M. 2009; World Health Organization ranking of antimicrobials according to their importance in human medicine: A critical step for developing risk management strategies for the use of antimicrobials in food production animals. Clin Infect Dis 49:132–141 [View Article][PubMed]
     [Google Scholar]
  5. D’Andrea M. M., Arena F., Pallecchi L., Rossolini G. M. 2013; CTX-M-type β-lactamases: a successful story of antibiotic resistance. Int J Med Microbiol 303:305–317 [View Article][PubMed]
     [Google Scholar]
  6. European Food Safety Authority 2013; The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2011. EFSA Journal 11:1–359
     [Google Scholar]
  7. Gilliver M. A., Bennett M., Begon M., Hazel S. M., Hart C. A. 1999; Antibiotic resistance found in wild rodents. Nature 401:233–234 [View Article][PubMed]
     [Google Scholar]
  8. Gómez Cano A. R., Hernández Fernández M., Alvarez-Sierra M. A. 2013; Dietary ecology of Murinae (Muridae, Rodentia): a geometric morphometric approach. PLoS ONE 8:e79080 [View Article][PubMed]
     [Google Scholar]
  9. Guenther S., Grobbel M., Beutlich J., Guerra B., Ulrich R. G., Wieler L. H., Ewers C. 2010; Detection of pandemic B2-O25-ST131 Escherichia coli harbouring the CTX-M-9 extended-spectrum beta-lactamase type in a feral urban brown rat (Rattus norvegicus). J Antimicrob Chemother 65:582–584 [View Article][PubMed]
     [Google Scholar]
  10. Guenther S., Bethe A., Fruth A., Semmler T., Ulrich R. G., Wieler L. H., Ewers C. 2012; Frequent combination of antimicrobial multiresistance and extraintestinal pathogenicity in Escherichia coli isolates from urban rats (Rattus norvegicus) in Berlin, Germany. PLoS ONE 7:e50331 [View Article][PubMed]
     [Google Scholar]
  11. Guenther S., Wuttke J., Bethe A., Vojtech J., Schaufler K., Semmler T., Ulrich R. G., Wieler L. H., Ewers C. 2013; Is fecal carriage of extended-spectrum-β-lactamase-producing Escherichia coli in urban rats a risk for public health?. Antimicrob Agents Chemother 57:2424–2425 [View Article][PubMed]
     [Google Scholar]
  12. Himsworth C. G., Parsons K. L., Jardine C., Patrick D. M. 2013; Rats, cities, people, and pathogens: a systematic review and narrative synthesis of literature regarding the ecology of rat-associated zoonoses in urban centers. Vector Borne Zoonotic Dis 13:349–359 [View Article][PubMed]
     [Google Scholar]
  13. Ho P. L., Tsang D. N., Que T. L., Ho M., Yuen K. Y. 2000; Comparison of screening methods for detection of extended-spectrum beta-lactamases and their prevalence among Escherichia coli and Klebsiella species in Hong Kong. APMIS 108:237–240 [View Article][PubMed]
     [Google Scholar]
  14. Ho P. L., Chow K. H., Lai E. L., Lo W. U., Yeung M. K., Chan J., Chan P. Y., Yuen K. Y. 2011a; Extensive dissemination of CTX-M-producing Escherichia coli with multidrug resistance to ‘critically important’ antibiotics among food animals in Hong Kong, 2008-10. J Antimicrob Chemother 66:765–768 [View Article][PubMed]
     [Google Scholar]
  15. Ho P. L., Lo W. U., Wong R. C., Yeung M. K., Chow K. H., Que T. L., Tong A. H., Bao J. Y., Lok S., Wong S. S. 2011b; Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong. J Antimicrob Chemother 66:752–756 [View Article][PubMed]
     [Google Scholar]
  16. Ho P. L., Lo W. U., Yeung M. K., Li Z., Chan J., Chow K. H., Yam W. C., Tong A. H., Bao J. Y. et al. 2012a; Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources. Vet Microbiol 158:172–179 [View Article][PubMed]
     [Google Scholar]
  17. Ho P. L., Yeung M. K., Lo W. U., Tse H., Li Z., Lai E. L., Chow K. H., To K. K., Yam W. C. 2012b; Predominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum beta-lactamase-producing Escherichia coli in Hong Kong, 1996-2008. Diagn Microbiol Infect Dis 73:182–186 [View Article][PubMed]
     [Google Scholar]
  18. Ho P. L., Chan J., Lo W. U., Law P. Y., Li Z., Lai E. L., Chow K. H. 2013; Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals. J Appl Microbiol 114:695–702 [View Article][PubMed]
     [Google Scholar]
  19. Kola A., Kohler C., Pfeifer Y., Schwab F., Kühn K., Schulz K., Balau V., Breitbach K., Bast A. et al. 2012; High prevalence of extended-spectrum-β-lactamase-producing Enterobacteriaceae in organic and conventional retail chicken meat, Germany. J Antimicrob Chemother 67:2631–2634 [View Article][PubMed]
     [Google Scholar]
  20. Lo W. U., Ho P. L., Chow K. H., Lai E. L., Yeung F., Chiu S. S. 2010; Fecal carriage of CTXM type extended-spectrum beta-lactamase-producing organisms by children and their household contacts. J Infect 60:286–292 [View Article][PubMed]
     [Google Scholar]
  21. Lo W. U., Chow K. H., Law P. Y., Ng K. Y., Cheung Y. Y., Lai E. L., Ho P. L. 2014; Highly conjugative IncX4 plasmids carrying blaCTX-M in Escherichia coli from humans and food animals. J Med Microbiol 63:835–840 [View Article][PubMed]
     [Google Scholar]
  22. Manges A. R., Johnson J. R. 2012; Food-borne origins of Escherichia coli causing extraintestinal infections. Clin Infect Dis 55:712–719 [View Article][PubMed]
     [Google Scholar]
  23. Meerburg B. G., Singleton G. R., Kijlstra A. 2009; Rodent-borne diseases and their risks for public health. Crit Rev Microbiol 35:221–270 [View Article][PubMed]
     [Google Scholar]
  24. Naseer U., Sundsfjord A. 2011; The CTX-M conundrum: dissemination of plasmids and Escherichia coli clones. Microb Drug Resist 17:83–97 [View Article][PubMed]
     [Google Scholar]
  25. Skurnik D., Ruimy R., Andremont A., Amorin C., Rouquet P., Picard B., Denamur E. 2006; Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli. J Antimicrob Chemother 57:1215–1219 [View Article][PubMed]
     [Google Scholar]
  26. Woerther P. L., Burdet C., Chachaty E., Andremont A. 2013; Trends in human fecal carriage of extended-spectrum β-lactamases in the community: toward the globalization of CTX-M. Clin Microbiol Rev 26:744–758 [View Article][PubMed]
     [Google Scholar]
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Clonal diversity of CTX-M-producing, multidrug-resistant Escherichia coli from rodents
J. Med. Microbiol. 64, 185 (2015); https://doi.org/10.1099/jmm.0.000001
/content/journal/jmm/10.1099/jmm.0.000001
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