1887

Journal of Medical Microbiology logo

Volume 64, Issue 4

Molecular identification and antifungal susceptibility of 186 isolates from vulvovaginal candidiasis in southern China Free

Abstract

There is limited information regarding the molecular epidemiology and antifungal susceptibilities of isolates using the Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18–50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and the D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using the Neo-Sensitabs tablet diffusion method. species were isolated from 186 cases (61.79 %). The most common pathogen was (91.4 %), followed by (4.3 %), (3.2 %) and (1.1 %). The susceptibility rates to were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole, ketoconazole and terbinafine (<0.01). The resistance rates to were 4.7, 6.5, 7.1, 7.6, 12.3, 27.7 and 74.7 % for caspofungin, miconazole, itraconazole, voriconazole, fluconazole, ketoconazole and terbinafine, respectively. No drugs tested apart from fluconazole exhibited differences in resistance between and non-albicans isolates. The results demonstrate that, using DNA sequencing, is the most common isolate from Chinese patients with VVC. Caspofungin, voriconazole and fluconazole may be preferable to other azoles and terbinafine in the treatment of VVC.

  • Received:
  • Accepted:
  • Published Online:
© 2015 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000024
2015-04-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/4/390.html?itemId=/content/journal/jmm/10.1099/jmm.0.000024&mimeType=html&fmt=ahah

References

  1. Chen S., Li S., Liu Z., Wu Y., Tu Y., Li J. 2007; Comparison of the effects of three different anti-fungus drugs on Candida albicans of murine vaginal mucosa. J Huazhong Univ Sci Technolog Med Sci 27:209–212 [View Article][PubMed]
     [Google Scholar]
  2. Espinel-Ingroff A., Canton E. 2008; Comparison of Neo-Sensitabs tablet diffusion assay with CLSI broth microdilution M38-A and disk diffusion methods for testing susceptibility of filamentous fungi with amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole. J Clin Microbiol 46:1793–1803 [View Article][PubMed]
     [Google Scholar]
  3. Ferahbas A., Koc A. N., Uksal U., Aygen E., Mistik S., Yildiz S. 2006; Terbinafine versus itraconazole and fluconazole in the treatment of Vulvovaginal candidiasis. Am J Ther 13:332–336 [View Article][PubMed]
     [Google Scholar]
  4. Gamarra S., Morano S., Dudiuk C., Mancilla E., Nardin M. E., de Los Angeles Méndez E., Garcia-Effron G. 2014; Epidemiology and antifungal susceptibilities of yeasts causing vulvovaginitis in a teaching hospital. Mycopathologia 178:251–258 [View Article][PubMed]
     [Google Scholar]
  5. Gross N. T., Arias M. L., Moraga M., Baddasarow Y., Jarstrand C. 2007; Species distribution and susceptibility to azoles of vaginal yeasts isolated prostitutes. Infect Dis Obstet Gynecol 2007:1–5 [View Article][PubMed]
     [Google Scholar]
  6. Ilkit M., Guzel A. B. 2011; The epidemiology, pathogenesis, and diagnosis of vulvovaginal candidosis: a mycological perspective. Crit Rev Microbiol 37:250–261 [View Article][PubMed]
     [Google Scholar]
  7. Kalkanci A., Güzel A. B., Jabban I. I., Aydin M., Ilkit M., Kuştimur S. 2013; Candida vaginitis in non-pregnant patients: a study of antifungal susceptibility testing and virulence factors. J Obstet Gynaecol 33:378–383 [View Article][PubMed]
     [Google Scholar]
  8. Konaté A., Yavo W., Kassi F. K., Djohan V., Angora E. K., Barro-Kiki P. C., Bosson-Vanga H., Soro F., Menan E. I. 2014; Aetiologies and contributing factors of vulvovaginal candidiasis in Abidjan (Cote d’Ivoire). J Mycol Med 24:93–99 [View Article][PubMed]
     [Google Scholar]
  9. Liu X. P., Fan S. R., Bai F. Y., Li J., Liao Q. P. 2009; Antifungal susceptibility and genotypes of Candida albicans strains from patients with vulvovaginal candidiasis. Mycoses 52:24–28 [View Article][PubMed]
     [Google Scholar]
  10. Liu X. P., Fan S. R., Peng Y. T., Zhang H. P. 2014; Species distribution and susceptibility of Candida isolates from patient with vulvovaginal candidiasis in Southern China from 2003 to 2012. J Mycol Med 24:106–111 [View Article][PubMed]
     [Google Scholar]
  11. Mahmoudabadi A. Z., Najafyan M., Moghimipour E., Alwanian M., Seifi Z. 2013; Lamisil versus clotrimazole in the treatment of vulvovaginal candidiasis. Iran J Microbiol 5:86–90[PubMed]
     [Google Scholar]
  12. Mahmoudi Rad M., Zafarghandi ASh., Amel Zabihi M., Tavallaee M., Mirdamadi Y. 2012; Identification of Candida species associated with vulvovaginal candidiasis by multiplex PCR. Infect Dis Obstet Gynecol 2012:1–5 [View Article][PubMed]
     [Google Scholar]
  13. Mendling W., Brasch J.German Society for Gynecology and Obstetrics Working Group for Infections and Infectimmunology in Gynecology and Obstetrics German Society of Dermatology, the Board of German DermatologistsGerman Speaking Mycological Society 2012; Guideline vulvovaginal candidosis (2010) of the German Society for Gynecology and Obstetrics, the Working Group for Infections and Infectimmunology in Gynecology and Obstetrics, the German Society of Dermatology, the Board of German Dermatologists and the German Speaking Mycological Society. Mycoses 55: Suppl. 3 1–13 [View Article][PubMed]
     [Google Scholar]
  14. Negri M., Henriques M., Svidzinski T. I., Paula C. R., Oliveira R. 2009; Correlation between Etest, disk diffusion, and microdilution methods for antifungal susceptibility testing of Candida species from infection and colonization. J Clin Lab Anal 23:324–330 [View Article][PubMed]
     [Google Scholar]
  15. Pfaller M. A., Castanheira M., Lockhart S. R., Ahlquist A. M., Messer S. A., Jones R. N. 2012; Frequency of decreased susceptibility and resistance to echinocandins among fluconazole-resistant bloodstream isolates of Candida glabrata. J Clin Microbiol 50:1199–1203 [View Article][PubMed]
     [Google Scholar]
  16. Trama J. P., Mordechai E., Adelson M. E. 2005; Detection and identification of Candida species associated with Candida vaginitis by real-time PCR and pyrosequencing. Mol Cell Probes 19:145–152 [View Article][PubMed]
     [Google Scholar]
  17. Vijaya D., Dhanalakshmi T. A., Kulkarni S. 2014; Changing trends of vulvovaginal candidiasis. J Lab Physicians 6:28–30 [View Article][PubMed]
     [Google Scholar]
  18. Yang Y. P., Li W., Huang W. M., Zhou Y., Fan Y. M. 2013; Chromoblastomycosis caused by Fonsecaea: clinicopathology, susceptibility and molecular identification of seven consecutive cases in Southern China. Clin Microbiol Infect 19:1023–1028 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000024
Loading
Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China
J. Med. Microbiol. 64, 390 (2015); https://doi.org/10.1099/jmm.0.000024
/content/journal/jmm/10.1099/jmm.0.000024
/content/journal/jmm/10.1099/jmm.0.000024
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error