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Volume 64, Issue 9

Isolation and characterization of a novel species, sp. nov., from common marmosets () Free

Abstract

Purpose-bred common marmosets from domestic sources housed in a US research facility, and used in multiple drug discovery programmes, were noted to have a high incidence of spontaneous inflammatory bowel disease and sporadic cholecystitis and cholangiohepatitis. Inflammatory infiltrates increased in incidence and severity with age. Because spp. have been linked to gastrointestinal diseases, samples from the gastrointestinal tracts of 39 marmosets were screened for spp. by culture and PCR. spp. were frequently detected in marmosets; 28.2 % of the marmosets were positive for a proposed novel species, sp. nov., by culture, and 48.7 % were positive by genus-specific PCR. Seventeen strains of sp. from 11 marmosets were cultured from various gastrointestinal sites. Older animals (age 6–11 years) had a higher helicobacter prevalence rate (57.1 %) compared with younger animals (age 3–5 years), which had a 27.2 % prevalence rate. Cells of sp. nov. were catalase, urease and oxidase positive and had fusiform morphology, with periplasmic fibres and multiple bipolar, sheathed flagella. All isolates had similar 16S and 23S rRNA sequences, which clustered as representatives of a novel species closely related to ‘ (97 %), a species isolated from cotton-top tamarins and ‘’ (96 %) isolated previously from the faeces of common marmosets. The whole genome sequence of one of the liver isolates, sp. nov. MIT 09-6949, had a 1.9 Mb genome length with a 41 mol% DNA G+C content. The type strain of sp. nov., MIT 09-6949, has been deposited in the BCCM/LMG Bacteria Collection as LMG 28613. These findings add to the increasing number of animal species with gastrointestinal disease in which novel enterohepatic spp. have been isolated.

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© 2015 The Authors
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2015-09-01
2024-04-20
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Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)
J. Med. Microbiol. 64, 1063 (2015); https://doi.org/10.1099/jmm.0.000113
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