1887

Abstract

Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray blood culture identification (BCID) panel (Biofire Diagnostics/bioMérieux) directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75 % with a Cohen κ value of 0.51. Global sensitivity and specificity using the FilmArray BCID panel were 71 and 97 %, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25 %), whereas the sensitivity for abscess samples was high (89 %). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost–benefit studies should be performed before introducing this method into algorithms for microbiological diagnostics.

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2015-12-01
2024-03-28
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References

  1. Almuhayawi M., Altun O., Strålin K., Ozenci V. 2014; Identification of microorganisms by FilmArray and matrix-assisted laser desorption ionization-time of flight mass spectrometry prior to positivity in the blood culture system. J Clin Microbiol 52:3230–3236 [View Article][PubMed]
    [Google Scholar]
  2. Altun O., Almuhayawi M., Ullberg M., Ozenci V. 2013; Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles. J Clin Microbiol 51:4130–4136 [View Article][PubMed]
    [Google Scholar]
  3. Bhatti M. M., Boonlayangoor S., Beavis K. G., Tesic V. 2014; Evaluation of FilmArray and Verigene systems for rapid identification of positive blood cultures. J Clin Microbiol 52:3433–3436 [View Article][PubMed]
    [Google Scholar]
  4. Bissonnette L., Bergeron M. G. 2006; Next revolution in the molecular theranostics of infectious diseases: microfabricated systems for personalized medicine. Expert Rev Mol Diagn 6:433–450 [View Article][PubMed]
    [Google Scholar]
  5. Blaschke A. J., Heyrend C., Byington C. L., Fisher M. A., Barker E., Garrone N. F., Thatcher S. A., Pavia A. T., Barney T., other authors. 2012; Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system. Diagn Microbiol Infect Dis 74:349–355 [View Article][PubMed]
    [Google Scholar]
  6. Carrara L., Navarro F., Turbau M., Serés M., Morán I., Quintana I., Martino R., González Y., Brell A., other authors. 2013; Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay. J Med Microbiol 62:1673–1679 [View Article][PubMed]
    [Google Scholar]
  7. CLSI 2012; Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  8. Esparcia O., Montemayor M., Ginovart G., Pomar V., Soriano G., Pericas R., Gurgui M., Sulleiro E., Prats G., other authors. 2011; Diagnostic accuracy of a 16S ribosomal DNA gene-based molecular technique (RT-PCR, microarray, and sequencing) for bacterial meningitis, early-onset neonatal sepsis, and spontaneous bacterial peritonitis. Diagn Microbiol Infect Dis 69:153–160 [View Article][PubMed]
    [Google Scholar]
  9. Ginocchio C. C., McAdam A. J. 2011; Current best practices for respiratory virus testing. J Clin Microbiol 49:(Suppl.)S44–S48 [View Article]
    [Google Scholar]
  10. Isenberg H. D. 2004 Clinical Microbiology Procedures Handbook, 2nd edn. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Khare R., Espy M. J., Cebelinski E., Boxrud D., Sloan L. M., Cunningham S. A., Pritt B. S., Patel R., Binnicker M. J. 2014; Comparative evaluation of two commercial multiplex panels for detection of gastrointestinal pathogens by use of clinical stool specimens. J Clin Microbiol 52:3667–3673 [View Article][PubMed]
    [Google Scholar]
  12. Lippi G., Danese E., Cervellin G., Montagnana M. 2014; Laboratory diagnostics of spontaneous bacterial peritonitis. Clin Chim Acta 430:164–170 [View Article][PubMed]
    [Google Scholar]
  13. Picard F. J., Bergeron M. G. 2002; Rapid molecular theranostics in infectious diseases. Drug Discov Today 7:1092–1101 [View Article][PubMed]
    [Google Scholar]
  14. Poritz M. A., Blaschke A. J., Byington C. L., Meyers L., Nilsson K., Jones D. E., Thatcher S. A., Robbins T., Lingenfelter B., other authors. 2011; FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection. PLoS One 6:e26047 [View Article][PubMed]
    [Google Scholar]
  15. Pozzetto B., Grattard F., Pillet S. 2010; Multiplex PCR theranostics of severe respiratory infections. Expert Rev Anti Infect Ther 8:251–253 [View Article][PubMed]
    [Google Scholar]
  16. Soriano G., Esparcia O., Montemayor M., Guarner-Argente C., Pericas R., Torras X., Calvo N., Román E., Navarro F., other authors. 2011; Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis. Aliment Pharmacol Ther 33:275–284 [View Article][PubMed]
    [Google Scholar]
  17. Tassi G. F., Marchetti G. P., Pinelli V., Chiari S. 2010; Practical management of pleural empyema. Monaldi Arch Chest Dis 73:124–129[PubMed]
    [Google Scholar]
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