1887

Abstract

spp. are a leading cause of bloodstream infection (BSI) and are associated with high mortality rates. Biofilm production is a virulence factor of spp., and has been linked with poor clinical outcome. The aim of our study was to assess biofilm production of bloodstream isolates at our institute, and to determine whether biofilm production is associated with any clinical characteristics of infection. During the four-year study period, 93 cases of BSI were analysed. The most frequently isolated species was (66.7 %), followed by (9.7 %), (9.7 %), (9.7 %) and (4.3 %). Biofilm production was more prevalent among non spp. (77.4 %) than (30.6 %) ( = 0.02). Abdominal surgery was identified as a risk factor of BSI caused by biofilm producing non- isolates. No risk factors predisposing to bloodstream infection caused by a biofilm producing isolate were identified. Biofilm production was not verified as a risk factor of mortality.

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2016-04-01
2024-04-16
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References

  1. Chandra J., Kuhn D. M., Mukherjee P. K., Hoyer L. L., McCormick T., Ghannoum M. A. 2001; Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol 183:5385–5394 [View Article][PubMed]
    [Google Scholar]
  2. Estivill D., Arias A., Torres-Lana A., Carrillo-Muñoz A. J., Arévalo M. P. 2011; Biofilm formation by five species of Candida on three clinical materials. J Microbiol Methods 86:238–242 [View Article][PubMed]
    [Google Scholar]
  3. Guembe M., Guinea J., Marcos-Zambrano L., Fernández-Cruz A., Peláez T., Muñoz P., Bouza E. 2014; Is biofilm production a predictor of catheter-related candidemia?. Med Mycol 52:407–410 [View Article][PubMed]
    [Google Scholar]
  4. Hii I. M., Chang H. L., Lin L. C., Lee Y. L., Liu Y. M., Liu C. E., Chen C. H., Cheng Y. R., Chang C. Y. 2015; Changing epidemiology of candidemia in a medical center in middle Taiwan. J Microbiol Immunol Infect 48:306–315 [View Article][PubMed]
    [Google Scholar]
  5. Kett D. H., Azoulay E., Echeverria P. M., Vincent J. L., Extended Prevalence of Infection in ICU Study (EPIC II) Group of Investigators. 2011; Candida bloodstream infections in intensive care units: analysis of the extended prevalence of infection in intensive care unit study. Crit Care Med 39:665–670 [View Article][PubMed]
    [Google Scholar]
  6. Kucharíková S., Tournu H., Holtappels M., Van Dijck P., Lagrou K. 2010; In vivo efficacy of anidulafungin against mature Candida albicans biofilms in a novel rat model of catheter-associated Candidiasis. Antimicrob Agents Chemother 54:4474–4475 [View Article][PubMed]
    [Google Scholar]
  7. Kuhn D. M., George T., Chandra J., Mukherjee P. K., Ghannoum M. A. 2002; Antifungal susceptibility of Candida biofilms: unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrob Agents Chemother 46:1773–1780 [View Article][PubMed]
    [Google Scholar]
  8. Kuhn D. M., Balkis M., Chandra J., Mukherjee P. K., Ghannoum M. A. 2003; Uses and limitations of the XTT assay in studies of Candida growth and metabolism. J Clin Microbiol 41:506–508 [View Article][PubMed]
    [Google Scholar]
  9. Kuhns M., Rosenberger A., Bader O., Reichard U., Gross U., Weig M. 2015; Incidence of candidaemia following abdominal surgery in German hospitals. Zentralbl Chir 140:617–623 [View Article][PubMed]
    [Google Scholar]
  10. Mukherjee P. K., Zhou G., Munyon R., Ghannoum M. A. 2005; Candida biofilm: a well-designed protected environment. Med Mycol 43:191–208 [View Article][PubMed]
    [Google Scholar]
  11. O'Toole G. A. 2011; Microtiter dish biofilm formation assay. J Vis Exp 47:2437[PubMed]
    [Google Scholar]
  12. Pannanusorn S., Fernandez V., Römling U. 2013; Prevalence of biofilm formation in clinical isolates of Candida species causing bloodstream infection. Mycoses 56:264–272 [View Article][PubMed]
    [Google Scholar]
  13. Puig-Asensio M., Padilla B., Garnacho-Montero J., Zaragoza O., Aguado J. M., Zaragoza R., Montejo M., Muñoz P., Ruiz-Camps I., other authors. 2014; Epidemiology and predictive factors for early and late mortality in Candida bloodstream infections: a population-based surveillance in Spain. Clin Microbiol Infect 20:O245–O254 [View Article][PubMed]
    [Google Scholar]
  14. Ramage G., Vande Walle K., Wickes B. L., López-Ribot J. L. 2001; Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 45:2475–2479 [View Article][PubMed]
    [Google Scholar]
  15. Shin J. H., Kee S. J., Shin M. G., Kim S. H., Shin D. H., Lee S. K., Suh S. P., Ryang D. W. 2002; Biofilm production by isolates of Candida species recovered from nonneutropenic patients: comparison of bloodstream isolates with isolates from other sources. J Clin Microbiol 40:1244–1248 [View Article][PubMed]
    [Google Scholar]
  16. Tumbarello M., Posteraro B., Trecarichi E. M., Fiori B., Rossi M., Porta R., de Gaetano Donati K., La Sorda M., Spanu T., other authors. 2007; Biofilm production by Candida species and inadequate antifungal therapy as predictors of mortality for patients with candidemia. J Clin Microbiol 45:1843–1850 [View Article][PubMed]
    [Google Scholar]
  17. Tumbarello M., Fiori B., Trecarichi E. M., Posteraro P., Losito A. R., De Luca A., Sanguinetti M., Fadda G., Cauda R., Posteraro B. 2012; Risk factors and outcomes of candidemia caused by biofilm-forming isolates in a tertiary care hospital. PLoS One 7:e33705 [View Article][PubMed]
    [Google Scholar]
  18. Uppuluri P., Chaturvedi A. K., Srinivasan A., Banerjee M., Ramasubramaniam A. K., Köhler J. R., Kadosh D., Lopez-Ribot J. L. 2010; Dispersion as an important step in the Candida albicans biofilm developmental cycle. PLoS Pathog 6:e1000828 [View Article][PubMed]
    [Google Scholar]
  19. Wisplinghoff H., Ebbers J., Geurtz L., Stefanik D., Major Y., Edmond M. B., Wenzel R. P., Seifert H. 2014; Nosocomial bloodstream infections due to Candida spp. in the USA: species distribution, clinical features and antifungal susceptibilities. Int J Antimicrob Agents 43:78–81 [View Article][PubMed]
    [Google Scholar]
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