1887

Abstract

Bovine mastitis is a costly disease in the dairy industry and does not always respond to antibiotic treatment. The major components of terpeneless, cold-pressed Valencia orange oil – citral, linalool, decanal and valencene – were examined as potential alternative treatments for associated mastitis. The minimum inhibitory concentration (MIC) of all four components against was determined after incubation for 24 h. Growth inhibition assays were performed for all effective components on for either a 3 h or 72 h treatment. These components were tested for the ability to disrupt pre-formed biofilms after 24 h of treatment by measuring absorbance at 540 nm. Cytotoxicity against immortalized bovine mammary epithelial (MAC-T) cells was measured using an MTT assay following a 1 h exposure. Only concentrations below the 50 % cytostatic concentration (CC50) were used in an adherence and invasion assay of on MAC-T cells, and for measurements of virulence and biofilm gene expression via qPCR. The MICs of citral and linalool were 0.02 % and 0.12 %, respectively, but decanal and valencene were ineffective. Citral and linalool were capable of inhibiting growth of after 24 h at their MIC values and inhibited pre-formed biofilms of . The concentrations below the CC50 were 0.02 % for citral and 0.12 % for linalool. These concentrations inhibited the adhesion and invasion ability of and downregulated virulence genes. Only 0.12 % linalool downregulated the expression of biofilm-forming genes. These components should be considered for further study.

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2016-07-01
2024-03-29
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