First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis
Carabarin-Lima, Alejandro and León-Izurieta, Libia and Rocha-Gracia, Rosa del Carmen and Castañeda-Lucio, Miguel and Torres, Carmen and Gutiérrez-Cazarez, Zita and González-Posos, Sirenia and Martínez de la Peña, Claudia F. and Martinez-Laguna, Ygnacio and Lozano-Zarain, Patricia,, 65, 729-737 (2016),
doi = https://doi.org/10.1099/jmm.0.000291,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential.,
language=,
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