1887

Journal of Medical Microbiology logo

Volume 65, Issue 9

Improved multilocus sequence typing of and closely related species Free

Abstract

The multilocus sequence typing (MLST) database (http://pubmlst.org/bpseudomallei/) contains the largest global sequence repository for and its closest genetic relatives. Using conventional MLST and MLST data derived from publicly available whole-genome sequences, we first defined the phylogenetic relatedness of and its nearest neighbours. Based on this analysis, we propose that the recently described complex (Bpc) should be expanded to encompass , (proposed), , , and three unassigned Clades A, B and C (represented by type strains BDU 5, BDU 8 and MSMB0265, respectively). Of note, the MLST locus is present in all Bpc species but is missing in all other spp., including all complex species, with the exception of most strains, which contain but encode genetically distinct sequences. The presence of is thus indicative of a Bpc strain. Next, we revisited the performance of the existing MLST primers, which prompted redesign of primers targeting the , , , and loci to encompass genetic diversity among Bpc strains and to address amplification/sequencing issues. We show and that the redesigned primers yield good-quality amplification and sequencing results for the , , , and loci in Bpc species. These primers provide an alternative for amplification and sequencing of MLST loci in Bpc species in cases when poor-quality amplification or sequencing data are obtained using the original MLST primers.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Burkholderia mallei , Burkholderia pseudomallei complex , Burkholderia thailandensis , MLST , multilocus sequence typing and sequence type
© 2016 The Authors
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2016-09-01
2024-04-20
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Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species
J. Med. Microbiol. 65, 992 (2016); https://doi.org/10.1099/jmm.0.000312
/content/journal/jmm/10.1099/jmm.0.000312
/content/journal/jmm/10.1099/jmm.0.000312
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