1887

Abstract

Infections and outbreaks caused by multidrug-resistant (MDR-AB) are prevalent and have been reported worldwide over the past 20 or more years. Class 1 integron in MDR-AB plays an important role in the spread of antibiotic resistance in clinical settings. This study has been conducted to evaluate the detection of metallo-β-lactamase, characterization of class 1 integron and determination of clonal relatedness among hospital isolates. Sixty-five clinical isolates of MDR-AB were recovered from two Iranian hospital’s intensive care units from February to August 2013. Integrase () and genes were detected in 70.8 % (=46/65) and 9.23 % (=6/65) of the isolates using PCR assay, respectively. No other metallo-β-lactamase genes ( , and ) were detected. PCR sequencing of integron gene cassette revealed the following arrays: - - (as a novel array was designated In), and . Analysis of gene revealed a new allele designated as . Gene transfer experiment by conjugation showed the 36 kb conjugative plasmid harbouring In The clonal assessment by repetitive extragenic palindromic PCR demonstrated a high-degree relatedness among the strains, but strains harbouring In displayed a different repetitive extragenic palindromic PCR profile. In this study, we found that a novel class 1 integron (In) that encoded a new allele resided on a transferable plasmid in non-clonal strains of MDR-AB.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000315
2016-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/928.html?itemId=/content/journal/jmm/10.1099/jmm.0.000315&mimeType=html&fmt=ahah

References

  1. Aktaş Z., Kayacan C. B. 2008; Investigation of metallo-beta-lactamase producing strains of Pseudomonas aeruginosa and Acinetobacter baumannii by E-test, disk synergy and PCR. Scand J Infect Dis 40:320–325 [View Article][PubMed]
    [Google Scholar]
  2. Arroyo L. A., García-Curiel A., Pachón-Ibañez M. E., Llanos A. C., Ruiz M., Pachón J., Aznar J. 2005; Reliability of the E-test method for detection of colistin resistance in clinical isolates of Acinetobacter baumannii . J Clin Microbiol 43:903–905 [View Article][PubMed]
    [Google Scholar]
  3. Asadollahi K., Taherikalani M., Maleki A., Alizadeh E., Valadbaigi H., Soroush S., Maleki H., Asadollahi P., Emaneini M. 2011; Diversity of aminoglycoside modifying enzyme genes among multidrug resistant Acinetobacter baumannii genotypes isolated from nosocomial infections in Tehran hospitals and their association with class 1 integrons. Acta Microbiol Immunol Hung 58:359–370 [View Article][PubMed]
    [Google Scholar]
  4. Bernabeu S., Poirel L., Nordmann P. 2012; Spectrophotometry-based detection of carbapenemase producers among Enterobacteriaceae . Diagn Microbiol Infect Dis 74:88–90 [View Article][PubMed]
    [Google Scholar]
  5. Bou G., Cerveró G., Domínguez M. A., Quereda C., Martínez-Beltrán J. 2000; PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii . Clin Microbiol Infect 6:635–643 [View Article][PubMed]
    [Google Scholar]
  6. Carattoli A. 2001; Importance of integrons in the diffusion of resistance. Vet Res 32:243–259 [View Article][PubMed]
    [Google Scholar]
  7. Carvalho K. R., Carvalho-Assef A. P., Peirano G., Santos L. C., Pereira M. J., Asensi M. D. 2009; Dissemination of multidrug-resistant Acinetobacter baumannii genotypes carrying bla OXA-23 collected from hospitals in Rio de Janeiro, Brazil. Int J Antimicrob Agents 34:25–28 [CrossRef]
    [Google Scholar]
  8. Dijkshoorn L., Nemec A., Seifert H. 2007; An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii . Nat Rev Microbiol 5:939–951 [View Article][PubMed]
    [Google Scholar]
  9. Fallah F., Borhan R. S., Hashemi A. 2013; Detection of bla(IMP) and bla(VIM) metallo-β-lactamases genes among Pseudomonas aeruginosa strains. Int J Burns Trauma 3:122–124[PubMed]
    [Google Scholar]
  10. Farshadzadeh Z., Hashemi F. B., Rahimi S., Pourakbari B., Esmaeili D., Haghighi M. A., Majidpour A., Shojaa S., Rahmani M. et al. 2015; Wide distribution of carbapenem resistant Acinetobacter baumannii in burns patients in Iran. Front Microbiol 6:11146 [View Article]
    [Google Scholar]
  11. Fournier P. E., Richet H., Weinstein R. A. 2006; The epidemiology and control of Acinetobacter baumannii in health care facilities. Clin Infect Dis 42:692–699 [View Article][PubMed]
    [Google Scholar]
  12. Gallego L., Towner K. J. 2001; Carriage of class 1 integrons and antibiotic resistance in clinical isolates of Acinetobacter baumannii from northern Spain. J Med Microbiol 50:71–77 [View Article][PubMed]
    [Google Scholar]
  13. Gaur A., Prakash P., Anupurba S., Mohapatra T. M. 2007; Possible role of integrase gene polymerase chain reaction as an epidemiological marker: study of multidrug-resistant Acinetobacter baumannii isolated from nosocomial infections. Int J Antimicrob Agents 29:446–450 [View Article][PubMed]
    [Google Scholar]
  14. Hu Z., Zhao W. H. 2009; Identification of plasmid- and integron-borne blaIMP-1 and blaIMP-10 in clinical isolates of Serratia marcescens . J Med Microbiol 58:217–221 [View Article][PubMed]
    [Google Scholar]
  15. Huang L. Y., Chen T. L., Lu P. L., Tsai C. A., Cho W. L., Chang F. Y., Fung C. P., Siu L. K. 2008; Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan. Clin Microbiol Infect 14:1010–1019 [View Article][PubMed]
    [Google Scholar]
  16. Japoni-Nejad A., Farshad S., vanm Belkum A., Ghaznavi-Rad E. 2013; Novel cassette array in a class 1 integron in clinical isolates of Acinetobacter baumannii from central Iran. Int J Med Microbiol 303:645–650 [View Article][PubMed]
    [Google Scholar]
  17. Juhas M., van der Meer J. R., Gaillard M., Harding R. M., Hood D. W., Crook D. W. 2009; Genomic islands: tools of bacterial horizontal gene transfer and evolution. FEMS Microbiol Rev 33:376–393 [View Article][PubMed]
    [Google Scholar]
  18. Koeleman J. G., Stoof J., Van Der Bijl M. W., Vandenbroucke-Grauls C. M., Savelkoul P. H. 2001; Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 39:8–13 [View Article][PubMed]
    [Google Scholar]
  19. Koornhof H. J., Keddy K., McGee L. 2001; Clonal expansion of bacterial pathogens across the world. J Travel Med 8:29–40 [View Article][PubMed]
    [Google Scholar]
  20. Laraki N., Franceschini N., Rossolini G. M., Santucci P., Meunier C., de Pauw E., Amicosante G., Frère J. M., Galleni M. 1999; Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-beta-lactamase IMP-1 produced by Escherichia coli . Antimicrob Agents Chemother 43:902–906[PubMed]
    [Google Scholar]
  21. Lin M. F., Chang K. C., Yang C. Y., Yang C. M., Xiao C. C., Kuo H. Y., Liou M. L. 2010; Role of integrons in antimicrobial susceptibility patterns of Acinetobacter baumannii . Jpn J Infect Dis 63:440–443[PubMed]
    [Google Scholar]
  22. Lin M. F., Lan C. Y. 2014; Antimicrobial resistance in Acinetobacter baumannii: from bench to bedside. World J Clin Cases 2:787 [View Article][PubMed]
    [Google Scholar]
  23. Manchanda V., Sanchaita S., Singh N. 2010; Multidrug resistant Acinetobacter . J Glob Infect Dis 2:291–304 [View Article][PubMed]
    [Google Scholar]
  24. Maragakis L. L., Perl T. M. 2008; Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis 46:1254–1263 [View Article][PubMed]
    [Google Scholar]
  25. Mendes R. E., Kiyota K. A., Monteiro J., Castanheira M., Andrade S. S., Gales A. C., Pignatari A. C. C., Tufik S. 2007; Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis. J Clin Microbiol 45:544–547 [View Article][PubMed]
    [Google Scholar]
  26. Misbah S., AbuBakar S., Hassan H., Hanifah Y. A., Yusof M. Y. 2004; Antibiotic susceptibility and REP-PCR fingerprints of Acinetobacter spp. isolated from a hospital ten years apart. J Hosp Infect 58:254–261 [View Article][PubMed]
    [Google Scholar]
  27. Naas T., Poirel L., Nordmann P. 2008; Minor extended-spectrum β-lactamases. Clin Microbiol Infect 14:42–52 [View Article][PubMed]
    [Google Scholar]
  28. Necati Hakyemez I., Kucukbayrak A., Tas T., Burcu Yikilgan A., Akkaya A., Yasayacak A., Akdeniz H. 2013; Nosocomial Acinetobacter baumannii infections and changing antibiotic resistance. Pak J Med Sci 29:1245–1248 [View Article][PubMed]
    [Google Scholar]
  29. Ploy M. C., Denis F., Courvalin P., Lambert T. 2000; Molecular characterization of integrons in Acinetobacter baumannii: description of a hybrid class 2 integron. Antimicrob Agents Chemother 44:2684–2688 [View Article][PubMed]
    [Google Scholar]
  30. Poirel L., Nordmann P. 2006; Carbapenem resistance in Acinetobacter baumannii: mechanisms and epidemiology. Clin Microbiol Infect 12:826–836 [View Article][PubMed]
    [Google Scholar]
  31. Ramirez M. S., Tolmasky M. E. 2010; Aminoglycoside modifying enzymes. Drug Resist Updat 13:151–171 [View Article][PubMed]
    [Google Scholar]
  32. Salimizand H., Menbari S., Ramazanzadeh R., Khonsha M., Vahedi M. S. 2014; DNA fingerprinting and antimicrobial susceptibility pattern of clinical and environmental Acinetobacter baumannii isolates: a multicentre study. J Chemother1973947814Y0000000227 [View Article][PubMed]
    [Google Scholar]
  33. Shahcheraghi F., Abbasalipour M., Feizabadi M., Ebrahimipour G., Akbari N. 2011; Isolation and genetic characterization of metallo-β-lactamase and carbapenamase producing strains of Acinetobacter baumannii from patients at Tehran hospitals. Iran J Microbiol 3:68–74[PubMed]
    [Google Scholar]
  34. Shakibaie M., Dhakephalker P., Kapadnis B., Salajaghe G., Chopade B. 1998; Plasmid mediated silver and antibiotic resistance in Acinetobacter baumannii BL54. Iran J Med Sci 23:30–36
    [Google Scholar]
  35. Sinirtaş M., Akalin H., Gedikoğlu S. 2009; Investigation of colistin sensitivity via three different methods in Acinetobacter baumannii isolates with multiple antibiotic resistance. Int J Infect Dis 13:e217220 [View Article][PubMed]
    [Google Scholar]
  36. Sung J. Y., Koo S. H., Kim S., Kwon K. C. 2014; Epidemiological characterizations of class 1 integrons from multidrug-resistant Acinetobacter isolates in Daejeon, Korea. Ann Lab Med 34:293–299 [View Article][PubMed]
    [Google Scholar]
  37. Székely E., Damjanova I., Jánvári L., Vas K. E., Molnár S., Bilca D. V., Lőrinczi L. K., Tóth A. 2013; First description of bla(NDM-1), bla(OXA-48), bla(OXA-181) producing Enterobacteriaceae strains in Romania . Int J Med Microbiol 303:697–700 [View Article][PubMed]
    [Google Scholar]
  38. Tanimoto K. 2013; Stenotrophomonas maltophilia strains isolated from a university hospital in Japan: genomic variability and antibiotic resistance. J Med Microbiol 62:565–570 [View Article][PubMed]
    [Google Scholar]
  39. Turton J. F., Kaufmann M. E., Glover J., Coelho J. M., Warner M., Pike R., Pitt T. L. 2005; Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom. J Clin Microbiol 43:3074–3082 [View Article][PubMed]
    [Google Scholar]
  40. Turton J. F., Woodford N., Glover J., Yarde S., Kaufmann M. E., Pitt T. L. 2006; Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol 44:2974–2976 [View Article][PubMed]
    [Google Scholar]
  41. Zarrilli R., Giannouli M., Tomasone F., Triassi M., Tsakris A. 2009; Carbapenem resistance in Acinetobacter baumannii: the molecular epidemic features of an emerging problem in health care facilities. J Infect Dev Ctries 3:335–341 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000315
Loading
/content/journal/jmm/10.1099/jmm.0.000315
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error