RT Journal Article SR Electronic(1) A1 Nguyen, Dong Tu A1 Ngo, Tuan Cuong A1 Le, Thanh Huong A1 Nguyen, Hoai Thu A1 Morita, Masatomo A1 Arakawa, Eiji A1 Ohnishi, Makoto A1 Nguyen, Binh Minh A1 Izumiya, HidemasaYR 2016 T1 Molecular epidemiology of Vibrio cholerae O1 in northern Vietnam (2007–2009), using multilocus variable-number tandem repeat analysis JF Journal of Medical Microbiology, VO 65 IS 9 SP 1007 OP 1012 DO https://doi.org/10.1099/jmm.0.000317 PB Microbiology Society, SN 1473-5644, AB Cholera is an infectious disease of major concern in Vietnam and other Asian countries. In 2009, there was a large outbreak of cholera in northern Vietnam. To investigate relationships among isolates of the causative pathogen Vibrio cholerae in this region since 2007, we carried out a multilocus variable-number tandem repeat analysis (MLVA) of 170 isolates collected between 2007 and 2009. A total of 24 MLVA types were identified using seven loci. Five clones (1–5) were identified using five loci of the large V. cholerae chromosome; clones 1 and 2 were major, and the others were minor. Clone 1 isolates were responsible for the 2009 outbreak. A shift in the predominant clone occurred between 2007 and 2009, with clone 1 likely derived from clone 2. Moreover, the former was less diverse than the latter, suggesting a single source of cholera dissemination. Epidemiological data indicated a wavelet prior to the large outbreak, suggesting that drinking water source or food chain became contaminated during dissemination. Our results reveal the utility of MLVA for analysis of V. cholerae isolates within a relatively short period and broaden our understanding of its transmission and response to cholera., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000317