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Volume 65, Issue 10

Characterization and evaluation of a novel immunochromatographic assay for pharyngeal ribosomal protein L7/L12 antigens Free

Abstract

Point-of-care testing for infection may be ideal and useful because significant numbers of the cases will be seen as outpatients. Recently, a new immunochromatographic method (ICM) targeting ribosomal protein L7/L12 (RP-L7/L12) in pharyngeal swabs became available in Japan, although clinical data and basic information regarding efficacy and characterization of this ICM are limited. The present study examined the fate of RP-L7/L12 during growth and the correlation between concentration in clinical specimens and the sensitivity of the ICM test. The usefulness of the ICM was investigated in patients suspected of having pneumonia and upper respiratory tract infection (137 children and 39 adults). The limit of detection for the ICM test was 1.1×10 c.f.u. ml of . Bacterial production of RP-L7/L12 correlated positively with the viable concentration ; antigen was then degraded in culture broth, with an half-life of approximately 2 days. Five other spp. and 14 representative respiratory pathogens were ICM assay negative at bacterial concentrations of 10 c.f.u. ml. The clinical sensitivity and specificity of the ICM assay were 57.1 % (20/35) and 92.2 % (130/141), respectively, in comparison with bacterial culture. Clinical specimens containing ≥10 c.f.u. ml of burden were ICM positive in 13 of 18 cases (72.2 %). The ICM is a poorly sensitive but reasonably specific means for detecting infections.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): characterization , Diagnosis , immune-chromatographic method , Mycoplasma pneumoniae , point-of-care-testing and ribosomal protein L7/L12
© 2016 The Authors
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2016-10-18
2024-04-16
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Characterization and evaluation of a novel immunochromatographic assay for pharyngeal Mycoplasmapneumoniae ribosomal protein L7/L12 antigens
J. Med. Microbiol. 65, 1105 (2016); https://doi.org/10.1099/jmm.0.000336
/content/journal/jmm/10.1099/jmm.0.000336
/content/journal/jmm/10.1099/jmm.0.000336
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