@article{mbs:/content/journal/jmm/10.1099/jmm.0.000357, author = "An, Jingna and Guo, Ling and Zhou, Lin and Ma, Yanning and Luo, Yanping and Tao, Chuanmin and Yang, Jiyong", title = "NDM-producing Enterobacteriaceae in a Chinese hospital, 2014–2015: identification of NDM-producing Citrobacterwerkmanii and acquisition of blaNDM-1-carrying plasmid in vivo in a clinical Escherichia coli isolate", journal= "Journal of Medical Microbiology", year = "2016", volume = "65", number = "11", pages = "1253-1259", doi = "https://doi.org/10.1099/jmm.0.000357", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000357", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Citrobacter werkmanii", keywords = "Enterobacteriaceae", keywords = "NDM-1", abstract = "New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 Escherichia coli, 10 Klebsiella pneumoniae, 9 Enterobacter cloacae, 2 Enterobacter aerogenes, 3 Providencia rettgeri, 1 Klebsiella oxytoca, 1 Proteus mirabilis, 1 Citrobacter freundii, 1 Citrobacter werkmanii and 1 Raoultella planticola were identified as NPE. Results of PFGE and multilocus sequence typing showed that most strains were genetically unrelated. Among the 45 bla NDM-carrying plasmids, there were 25 IncX3 plasmids with a size of about 30 to 50 kb, one 100 kb IncX3 plasmid, 11 IncA/C plasmids with a size range from 70 to 300 kb, six 90 to 120 kb IncB/O plasmids, one IncN plasmid with a size of 100 kb and one 140 kb IncFrep plasmid. An NDM-1-producing isolate of C. werkmanii was identified, which had not been reported previously. An Escherichia coli strain was found acquiring a bla NDM-1-carrying IncFrep plasmid in vivo during infection. In conclusion, an NDM-1-producing isolate of C. werkmanii was identified. An Escherichia coli strain acquired a bla NDM-1-carrying plasmid in vivo. IncX3 and IncA/C plasmids with various sizes might have emerged as the main platforms mediating the spread of the bla NDM genes in China.", }