1887

Journal of Medical Microbiology logo

Volume 65, Issue 11

Structures and serospecificity of threonine-containing O polysaccharides of two clinical isolates belonging to the genus and their classification into O11 subserogroups Free

Abstract

Two clinical isolates from Polish patients, 9B-m and genomospecies 3J-r, were found to be serologically related to O11. However, serological studies involving ELISA and Western blotting methods, using lipopolysaccharides (LPSs) extracted from the strains as antigens and native or adsorbed rabbit polyclonal O antisera, specific to the studied strains, revealed slight differences in the cross-reactivity and specificity of the two studied isolates, when compared to O11. Two different O polysaccharides containing -(-galacturonoyl)--threonine were isolated from the LPSs of the isolates. Their structures were determined by chemical analysis and NMR spectroscopy and found to be related to the O11 antigen structure established earlier, the 9B-m structure differing in the absence of the lateral glucose residue and the 3J-r structure in non-stoichiometric -acetylation of the threonine residue only. Thus, the O11 serogroup should be divided into two subgroups: O11a, represented by the 9B-m isolate and O11a, b possessing the additional b epitope, containing the lateral residue of glucose and formed by the 3J-r isolate as well as 25/57 belonging to O11 serogroup so far. O11a is the sixth new serotype found in spp. strains recently isolated from patients in central Poland.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial polysaccharide structure , O antigen , Proteus , serological classification and threonine
© 2016 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000360
2016-11-16
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/11/1260.html?itemId=/content/journal/jmm/10.1099/jmm.0.000360&mimeType=html&fmt=ahah

References

  1. Arbatsky N. P., Shashkov A. S., Literacka E., Widmalm G., Kaca W., Knirel Y. A. 2000; Structure of the O-specific polysaccharide of Proteus mirabilis O11, another Proteus O-antigen containing an amide of d-galacturonic acid with l-threonine. Carbohydr Res 323:81–86 [View Article][PubMed]
     [Google Scholar]
  2. Arbatsky N. P., Drzewiecka D., Palusiak A., Shashkov A. S., Zabłotni A., Siwińska M., Knirel Y. A. 2013; Structure of a Kdo-containing O polysaccharide representing Proteus O79, a newly described serogroup for some clinical Proteus genomospecies isolates from Poland. Carbohydr Res 379:100–105 [View Article][PubMed]
     [Google Scholar]
  3. Behrendt U., Augustin J., Spröer C., Gelbrecht J., Schumann P., Ulrich A. 2015; Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium. Antonie Van Leeuwenhoek 108:1457–1468 [View Article][PubMed]
     [Google Scholar]
  4. Bock K., Pedersen C. 1983; Carbon-13 nuclear magnetic resonance spectroscopy of monosaccharides. Adv Carbohydr Chem Biochem 41:27–65 [CrossRef]
     [Google Scholar]
  5. Drzewiecka D. 2016; Significance and roles of Proteus bacteria in natural environments. Microbiol Ecol 72:741–758 [CrossRef]
     [Google Scholar]
  6. Drzewiecka D., Arbatsky N. P., Shashkov A. S., Staczek P., Knirel Y. A., Sidorczyk Z. 2008; Structure and serological properties of the O-antigen of two clinical Proteus mirabilis strains classified into a new Proteus O77 serogroup. FEMS Immunol Med Microbiol 54:185–194 [View Article][PubMed]
     [Google Scholar]
  7. Drzewiecka D., Arbatsky N. P., Staczek P., Shashkov A. S., Knirel Y. A., Sidorczyk Z. 2010; Structural and serological studies of the O-polysaccharide of strains from a newly created Proteus O78 serogroup prevalent in Polish patients. FEMS Immunol Med Microbiol 58:269–276 [View Article][PubMed]
     [Google Scholar]
  8. Drzewiecka D., Shashkov A. S., Arbatsky N. P., Knirel Y. A. 2016; Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland. Microbiology 162:789–797 [View Article][PubMed]
     [Google Scholar]
  9. Janda J. M., Abbott S. L., Khashe S., Probert W. 2001; Biochemical identification and characterization of DNA groups within the Proteus vulgaris complex. J Clin Microbiol 39:1231–1234 [View Article][PubMed]
     [Google Scholar]
  10. Knirel Y. A., Zych K., Vinogradov E., Shashkov A. S., Sidorczyk Z. 2000; Structure of a 2-aminoethyl phosphate-containing O-specific polysaccharide of Proteus penneri 8 from a new serogroup O67. Eur J Biochem 267:815–820 [View Article][PubMed]
     [Google Scholar]
  11. Knirel Y. A., Perepelov A. V., Kondakova A. N., Senchenkova S. N., Sidorczyk Z., Rozalski A., Kaca W. 2011; Structure and serology of O-antigens as the basis for classification of Proteus strains. Innate Immun 17:70–96 [View Article][PubMed]
     [Google Scholar]
  12. Leontein K., Lindberg B., Lōnngren J. 1978; Assignment of absolute configuration of sugars by g.l.c. of their acetylated glycosides formed from chiral alcohols. Carbohydr Res 62:359–362 [View Article]
     [Google Scholar]
  13. O'Hara C. M., Brenner F. W., Miller J. M. 2000a; Classification, identification, and clinical significance of Proteus, Providencia, and Morganella . Clin Microbiol Rev 13:534–546 [View Article][PubMed]
     [Google Scholar]
  14. O'Hara C. M., Brenner F. W., Steigerwalt A. G., Hill B. C., Holmes B., Grimont P. A., Hawkey P. M., Penner J. L., Miller J. M., Brenner D. J. 2000b; Classification of Proteus vulgaris biogroup 3 with recognition of Proteus hauseri sp. nov., nom. rev. and unnamed Proteus genomospecies 4, 5 and 6. Int J Syst Evol Microbiol 50:1869–1875 [View Article][PubMed]
     [Google Scholar]
  15. Ratnayake S., Weintraub A., Widmalm G. 1994; Structural studies of the enterotoxigenic Escherichia coli (ETEC) O153 O-antigenic polysaccharide. Carbohydr Res 265:113–120 [View Article][PubMed]
     [Google Scholar]
  16. Różalski A., Torzewska A., Moryl M., Kwil I., Maszewska A., Ostrowska K., Drzewiecka D., Zabłotni A., Palusiak A. et al. 2012; Proteus sp. – an opportunistic bacterial pathogen – classification, swarming growth, clinical significance and virulence factors. Folia Biol Oecol 8:1–17 [View Article]
     [Google Scholar]
  17. Senior B. W. 1997; Media and tests to simplify the recognition and identification of members of the Proteeae . J Med Microbiol 46:39–44 [View Article][PubMed]
     [Google Scholar]
  18. Sidorczyk Z., Swierzko A., Knirel Y. A., Vinogradov E. V., Chernyak A. Y., Kononov L. O., Cedzynski M., Rozalski A., Kaca W., Shashkov A. S. 1995; Structure and epitope specificity of the O-specific polysaccharide of Proteus penneri strain 12 (ATCC 33519) containing the amide of d-galacturonic acid with l-threonine. Eur J Biochem 230:713–721 [View Article][PubMed]
     [Google Scholar]
  19. Sidorczyk Z., Zych K., Toukach F. V., Arbatsky N. P., Zablotni A., Shashkov A. S., Knirel Y. A. 2002; Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3. Eur J Biochem 269:1406–1412 [View Article][PubMed]
     [Google Scholar]
  20. Siwińska M., Levina E. A., Ovchinnikova O. G., Drzewiecka D., Shashkov A. S., Różalski A., Knirel Y. A. 2015; Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80. Carbohydr Res 407:131–136 [View Article][PubMed]
     [Google Scholar]
  21. Warren J. W. 1996; Clinical presentation and epidemiology of urinary tract infections. In Urinary Tract Infections. Molecular Pathogenesis and Clinical Management pp 2–28 Edited by Mobley H. T. L., Warren J. W. Washington, DC: ASM Press;
     [Google Scholar]
  22. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5:83–89
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000360
Loading
Structures and serospecificity of threonine-containing O polysaccharides of two clinical isolates belonging to the genus Proteus and their classification into O11 subserogroups
J. Med. Microbiol. 65, 1260 (2016); https://doi.org/10.1099/jmm.0.000360
/content/journal/jmm/10.1099/jmm.0.000360
/content/journal/jmm/10.1099/jmm.0.000360
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error